5HT2C Receptor Antibody | Affinity Biosciences

WB
IF/ICC
Cites

1/6

Western blot analysis of extracts from Mouse testis, using 5HT2C Receptor Antibody at 1/1000 dilution.

Western blot analysis of extracts from EC304 cells(heat-shock treatment), using 5HT2C Receptor Antibody at 1/1000 dilution.

DF3501 staining Hela cells by IF/ICC.

Fig.

Product:	5HT2C Receptor Antibody
Catalog:	DF3501
Description:	Rabbit polyclonal antibody to 5HT2C Receptor
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Horse, Sheep, Rabbit, Dog
Mol.Wt.:	55 KD; 52kD(Calculated).
Uniprot:	P28335
RRID:	AB_2835863

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(91%), Horse(91%), Sheep(91%), Rabbit(91%), Dog(100%)

Clonality:

Polyclonal

Specificity:

5HT2C Receptor Antibody detects endogenous levels of total 5HT2C Receptor.

RRID:

AB_2835863
Cite Format: Affinity Biosciences Cat# DF3501, RRID:AB_2835863.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

5 Hydroxytryptamine 2C receptor; 5-HT-1C; 5-ht-1c receptor; 5-HT-2C; 5-HT1C; 5-HT2C; 5-HTR2C; 5-hydroxytryptamine (serotonin) receptor 2C, G protein-coupled; 5-hydroxytryptamine receptor 1C; 5-hydroxytryptamine receptor 2C; 5HT1C; 5HT2C; 5HT2C_HUMAN; 5HTR2C; 5Hydroxytryptamine 2C receptor; Htr1c; HTR2C; serotonin 1c receptor; serotonin 2c receptor; Serotonin 5-HT-2C receptor; Serotonin receptor 2C;

Immunogens

Immunogen:

Uniprot:

P28335(5HT2C_HUMAN) >>Visit HPA database.

Gene(ID):

HTR2C(3358)

Expression:

P28335 5HT2C_HUMAN:

Detected in brain.

Sequence:

P28335 5HT2C_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MVNLRNAVHSFLVHLIGLLVWQCDISVSPVAAIVTDIFNTSDGGRFKFPDGVQNWPALSIVIIIIMTIGGNILVIMAVSMEKKLHNATNYFLMSLAIADMLVGLLVMPLSLLAILYDYVWPLPRYLCPVWISLDVLFSTASIMHLCAISLDRYVAIRNPIEHSRFNSRTKAIMKIAIVWAISIGVSVPIPVIGLRDEEKVFVNNTTCVLNDPNFVLIGSFVAFFIPLTIMVITYCLTIYVLRRQALMLLHGHTEEPPGLSLDFLKCCKRNTAEEENSANPNQDQNARRRKKKERRPRGTMQAINNERKASKVLGIVFFVFLIMWCPFFITNILSVLCEKSCNQKLMEKLLNVFVWIGYVCSGINPLVYTLFNKIYRRAFSNYLRCNYKVEKKPPVRQIPRVAATALSGRELNVNIYRHTNEPVIEKASDNEPGIEMQVENLELPVNPSSVVSERISSV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Dog

100

Pig

Horse

Sheep

Rabbit

Xenopus

Chicken

Bovine

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P28335 As Substrate

P28335

Site	PTM Type	Source
N39	N-Glycosylation	Uniprot
K391	Acetylation	Uniprot
S456	Phosphorylation	Uniprot
S457	Phosphorylation	Uniprot

Research Backgrounds

P28335_5HT2C_HUMAN

Function:

G-protein coupled receptor for 5-hydroxytryptamine (serotonin). Also functions as a receptor for various drugs and psychoactive substances, including ergot alkaloid derivatives, 1-2,5,-dimethoxy-4-iodophenyl-2-aminopropane (DOI) and lysergic acid diethylamide (LSD). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Beta-arrestin family members inhibit signaling via G proteins and mediate activation of alternative signaling pathways. Signaling activates a phosphatidylinositol-calcium second messenger system that modulates the activity of phosphatidylinositol 3-kinase and down-stream signaling cascades and promotes the release of Ca(2+) ions from intracellular stores. Regulates neuronal activity via the activation of short transient receptor potential calcium channels in the brain, and thereby modulates the activation of pro-opiomelacortin neurons and the release of CRH that then regulates the release of corticosterone. Plays a role in the regulation of appetite and eating behavior, responses to anxiogenic stimuli and stress. Plays a role in insulin sensitivity and glucose homeostasis.

PTMs:

N-glycosylated.

Subcellular Location:

Cell membrane>Multi-pass membrane protein.

Tissue Specificity:

Detected in brain.

Subunit Structure:

Interacts with MPDZ. Interacts with ARRB2.

Family&Domains:

The PDZ domain-binding motif is involved in the interaction with MPDZ.

Belongs to the G-protein coupled receptor 1 family.

Research Fields

· Cellular Processes > Cellular community - eukaryotes > Gap junction. (View pathway)

· Environmental Information Processing > Signal transduction > Calcium signaling pathway. (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > Neuroactive ligand-receptor interaction.

· Organismal Systems > Nervous system > Serotonergic synapse.

· Organismal Systems > Sensory system > Inflammatory mediator regulation of TRP channels. (View pathway)

References

1). Agomelatine rescues lipopolysaccharide-induced neural injury and depression-like behaviors via suppression of the Gαi-2-PKA-ASK1 signaling pathway. Journal of Neuroinflammation (PubMed: 35610704) [IF=9.3]

Application: IF/ICC Species: Rat Sample:

Fig. 2 Agomelatine appears to exert antidepressant effects through regulation of downstream pathways involving interactions of the 5-HT2C reporter and Gαi-2. A Representative images of the 5-HT2C receptor (red). Nuclei (blue) are stained with DAPI. Neurons (green) are stained with Anti-NeuN. Scale bar is 5 μm (N = 6 per group). B Representative images of laser scanning confocal microscopy of the 5-HT2C receptor (red) and Gαi-2 (green) in the hippocampal DG region. C CO-IP analysis of endogenous interactions between the 5-HT2C receptor and Gαi-2 in the DG region. D ELISA analysis of cAMP in the DG region of LPS-treated rats receiving a pretreatment of agomelatine. E Western blot assays of protein expression levels of the 5-HT2C receptor, Gαi-2, P-PKA and P-ASK1within the DG region (N = 6 per group). *P

Application: WB Species: Rat Sample:

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5HT2C Receptor Antibody - #DF3501