TFE3 Antibody - #AF0363

(3)  
Product: TFE3 Antibody
Catalog: AF0363
Description: Rabbit polyclonal antibody to TFE3
Application: WB IHC IF/ICC
Reactivity: Human, Mouse
Prediction: Pig, Bovine, Horse, Sheep, Dog
Mol.Wt.: 62kDa; 62kD(Calculated).
Uniprot: P19532
RRID: AB_2833528

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
TFE3 Antibody detects endogenous levels of total TFE3.
RRID:
AB_2833528
Cite Format: Affinity Biosciences Cat# AF0363, RRID:AB_2833528.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

bHLH e33; bHLHe33; Class E basic helix-loop-helix protein 33; RCCP 2; RCCP2; RCCX1; Renal cell carcinoma, papillary; Tcfe 3; Tcfe3; TFE 3; Tfe3; TFE3_HUMAN; TFEA; Transcription factor binding to IGHM enhancer 3; Transcription factor E family, member A; Transcription factor E3; Transcription factor for IgH enhancer; Transcription factor for immunoglobulin heavy chain enhancer 3;

Immunogens

Immunogen:
Uniprot:

P19532(TFE3_HUMAN) >>Visit HPA database.

Gene(ID):
TFE3(7030)
Expression:
P19532 TFE3_HUMAN:

Ubiquitous in fetal and adult tissues.

Description:
TFE3 Transcription factor that specifically recognizes and binds E-box sequences (3'-CANNTG-5'). Efficient DNA-binding requires dimerization with itself or with another MiT/TFE family member such as TFEB or MITF. In association with TFEB, activates the expression of CD40L in T-cells, thereby playing a role in T- cell-dependent antibody responses in activated CD4(+) T-cells and thymus-dependent humoral immunity. Specifically recognizes the MUE3 box, a subset of E-boxes, present in the immunoglobulin enhancer.
Sequence:
MSHAAEPARDGVEASAEGPRAVFVLLEERRPADSAQLLSLNSLLPESGIVADIELENVLDPDSFYELKSQPLPLRSSLPISLQATPATPATLSASSSAGGSRTPAMSSSSSSRVLLRQQLMRAQAQEQERRERREQAAAAPFPSPAPASPAISVVGVSAGGHTLSRPPPAQVPREVLKVQTHLENPTRYHLQQARRQQVKQYLSTTLGPKLASQALTPPPGPASAQPLPAPEAAHTTGPTGSAPNSPMALLTIGSSSEKEIDDVIDEIISLESSYNDEMLSYLPGGTTGLQLPSTLPVSGNLLDVYSSQGVATPAITVSNSCPAELPNIKREISETEAKALLKERQKKDNHNLIERRRRFNINDRIKELGTLIPKSSDPEMRWNKGTILKASVDYIRKLQKEQQRSKDLESRQRSLEQANRSLQLRIQELELQAQIHGLPVPPTPGLLSLATTSASDSLKPEQLDIEEEGRPGAATFHVGGGPAQNAPHQQPPAPPSDALLDLHFPSDHLGDLGDPFHLGLEDILMEEEEGVVGGLSGGALSPLRAASDPLLSSVSPAVSKASSRRSSFSMEEES

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P19532 As Substrate

Site PTM Type Enzyme
S2 Phosphorylation
K68 Sumoylation
T85 Phosphorylation
S101 Phosphorylation
S109 Phosphorylation
S112 Phosphorylation
R122 Methylation
S149 Phosphorylation
K178 Methylation
K200 Ubiquitination
Y202 Phosphorylation
T217 Phosphorylation
S246 Phosphorylation
S321 Phosphorylation
K330 Sumoylation
S334 Phosphorylation
K339 Ubiquitination
R365 Methylation
K385 Ubiquitination
K401 Acetylation
S542 Phosphorylation
S548 Phosphorylation
S554 Phosphorylation
S556 Phosphorylation
S560 Phosphorylation
S563 Phosphorylation
S564 Phosphorylation
S567 Phosphorylation
S568 Phosphorylation
S570 Phosphorylation
S575 Phosphorylation

Research Backgrounds

Function:

Transcription factor that specifically recognizes and binds E-box sequences (5'-CANNTG-3') (By similarity). Efficient DNA-binding requires dimerization with itself or with another MiT/TFE family member such as TFEB or MITF (By similarity). In association with TFEB, activates the expression of CD40L in T-cells, thereby playing a role in T-cell-dependent antibody responses in activated CD4(+) T-cells and thymus-dependent humoral immunity (By similarity). Specifically recognizes the MUE3 box, a subset of E-boxes, present in the immunoglobulin enhancer. It also binds very well to a USF/MLTF site. May regulate lysosomal positioning in response to nutrient deprivation by promoting the expression of PIP4P1.

PTMs:

Sumoylated; does not affect dimerization with MITF.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitous in fetal and adult tissues.

Subunit Structure:

Homodimer and heterodimer; with TFEB or MITF.

Family&Domains:

Belongs to the MiT/TFE family.

Research Fields

· Human Diseases > Cancers: Overview > Transcriptional misregulation in cancer.

· Human Diseases > Cancers: Specific types > Renal cell carcinoma.   (View pathway)

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