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$350 200ul

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  • Product Name
    TFE3 Antibody
  • Catalog No.
    AF0363
  • RRID
    AB_2833528
  • Source
    Rabbit
  • Application
    WB,IHC,IF/ICC,ELISA(peptide)
  • Reactivity
    Human, Mouse
  • Prediction
    Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%)
  • UniProt
  • Mol.Wt
    62kD;
    62kDa(Calculated).
  • Concentration
    1mg/ml
  • Browse similar products>>

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Product Information

Alternative Names:Expand▼

bHLH e33; bHLHe33; Class E basic helix-loop-helix protein 33; RCCP 2; RCCP2; RCCX1; Renal cell carcinoma, papillary; Tcfe 3; Tcfe3; TFE 3; Tfe3; TFE3_HUMAN; TFEA; Transcription factor binding to IGHM enhancer 3; Transcription factor E family, member A; Transcription factor E3; Transcription factor for IgH enhancer; Transcription factor for immunoglobulin heavy chain enhancer 3;

Applications:

WB: 1:500-1:3000, IHC: 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000
*The optimal dilutions should be determined by the end user.

Reactivity:

Human, Mouse

Predicted Reactivity:

Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%)

Source:

Rabbit

Clonality:

Polyclonal

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Specificity:

TFE3 Antibody detects endogenous levels of total TFE3.

RRID:

AB_2833528
Please cite this product as: Affinity Biosciences Cat# AF0363, RRID:AB_2833528.

Format:

Liquid

Concentration:

1mg/ml

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

Immunogen Information

Immunogen:

A synthesized peptide derived from human TFE3, corresponding to a region within the internal amino acids.

Uniprot:



>>Visit The Human Protein Atlas

Gene ID:

Gene Name:

TFE3

Molecular Weight:

Observed Mol.Wt.: 62kD.
Predicted Mol.Wt.: 62kDa(Calculated)..

Subcellular Location:

Nucleus.

Tissue Specificity:

P19532 TFE3_HUMAN:
Ubiquitous in fetal and adult tissues.

Description:

TFE3 Transcription factor that specifically recognizes and binds E-box sequences (3'-CANNTG-5'). Efficient DNA-binding requires dimerization with itself or with another MiT/TFE family member such as TFEB or MITF. In association with TFEB, activates the expression of CD40L in T-cells, thereby playing a role in T- cell-dependent antibody responses in activated CD4(+) T-cells and thymus-dependent humoral immunity. Specifically recognizes the MUE3 box, a subset of E-boxes, present in the immunoglobulin enhancer.

Sequence:
MSHAAEPARDGVEASAEGPRAVFVLLEERRPADSAQLLSLNSLLPESGIVADIELENVLDPDSFYELKSQPLPLRSSLPISLQATPATPATLSASSSAGGSRTPAMSSSSSSRVLLRQQLMRAQAQEQERRERREQAAAAPFPSPAPASPAISVVGVSAGGHTLSRPPPAQVPREVLKVQTHLENPTRYHLQQARRQQVKQYLSTTLGPKLASQALTPPPGPASAQPLPAPEAAHTTGPTGSAPNSPMALLTIGSSSEKEIDDVIDEIISLESSYNDEMLSYLPGGTTGLQLPSTLPVSGNLLDVYSSQGVATPAITVSNSCPAELPNIKREISETEAKALLKERQKKDNHNLIERRRRFNINDRIKELGTLIPKSSDPEMRWNKGTILKASVDYIRKLQKEQQRSKDLESRQRSLEQANRSLQLRIQELELQAQIHGLPVPPTPGLLSLATTSASDSLKPEQLDIEEEGRPGAATFHVGGGPAQNAPHQQPPAPPSDALLDLHFPSDHLGDLGDPFHLGLEDILMEEEEGVVGGLSGGALSPLRAASDPLLSSVSPAVSKASSRRSSFSMEEES

Research Background

Function:

Transcription factor that specifically recognizes and binds E-box sequences (5'-CANNTG-3') (By similarity). Efficient DNA-binding requires dimerization with itself or with another MiT/TFE family member such as TFEB or MITF (By similarity). In association with TFEB, activates the expression of CD40L in T-cells, thereby playing a role in T-cell-dependent antibody responses in activated CD4(+) T-cells and thymus-dependent humoral immunity (By similarity). Specifically recognizes the MUE3 box, a subset of E-boxes, present in the immunoglobulin enhancer. It also binds very well to a USF/MLTF site. May regulate lysosomal positioning in response to nutrient deprivation by promoting the expression of PIP4P1.

Post-translational Modifications:

Sumoylated; does not affect dimerization with MITF.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte

Tissue Specificity:

Ubiquitous in fetal and adult tissues.

Subunit Structure:

Homodimer and heterodimer; with TFEB or MITF.

Similarity:

Belongs to the MiT/TFE family.

Research Fields

Research Fields:

· Human Diseases > Cancers: Specific types > Renal cell carcinoma.(View pathway)
· Human Diseases > Cancers: Overview > Transcriptional misregulation in cancer.

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Catalog Number :

AF0363-BP
(Blocking peptide available as AF0363-BP)

Price/Size :

$350/1mg.
Tips: For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Function :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

Format and storage :

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.

Precautions :

This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Pig
100%
Horse
100%
Bovine
100%
Sheep
100%
Dog
100%
Xenopus
0%
Zebrafish
0%
Chicken
0%
Rabbit
0%
High similarity Medium similarity Low similarity No similarity
P19532 as Substrate
Site PTM Type Enzyme
S2 Phosphorylation
K68 Sumoylation
T85 Phosphorylation
S101 Phosphorylation
S109 Phosphorylation
S112 Phosphorylation
R122 Methylation
S149 Phosphorylation
K178 Methylation
K200 Ubiquitination
Y202 Phosphorylation
T217 Phosphorylation
S246 Phosphorylation
S321 Phosphorylation
K330 Sumoylation
S334 Phosphorylation
K339 Ubiquitination
R365 Methylation
K385 Ubiquitination
K401 Acetylation
S542 Phosphorylation
S548 Phosphorylation
S554 Phosphorylation
S556 Phosphorylation
S560 Phosphorylation
S563 Phosphorylation
S564 Phosphorylation
S567 Phosphorylation
S568 Phosphorylation
S570 Phosphorylation
S575 Phosphorylation
IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.

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