AARSD1 Antibody | Affinity Biosciences

WB
IF/ICC

1/2

Western blot analysis of extracts from various samples, using AARSD1 Antibody.

Product:	AARSD1 Antibody
Catalog:	DF3751
Description:	Rabbit polyclonal antibody to AARSD1
Application:	WB IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine
Mol.Wt.:	45 KD; 45kD(Calculated).
Uniprot:	Q9BTE6
RRID:	AB_2836115

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(100%)

Clonality:

Polyclonal

Specificity:

AARSD1 Antibody detects endogenous levels of total AARSD1.

RRID:

AB_2836115
Cite Format: Affinity Biosciences Cat# DF3751, RRID:AB_2836115.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Aarsd1; AASD1_HUMAN; Alanyl-tRNA editing protein Aarsd1; Alanyl-tRNA synthetase domain containing 1; Alanyl-tRNA synthetase domain-containing protein 1; MGC2744; OTTHUMP00000204017; OTTHUMP00000204019; OTTHUMP00000204020;

Immunogens

Immunogen:

Uniprot:

Q9BTE6(AASD1_HUMAN) >>Visit HPA database.

Gene(ID):

AARSD1(100885850)

Sequence:

Q9BTE6 AASD1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAFWCQRDSYAREFTTTVVSCCPAELQTEGSNGKKEVLSGFQVVLEDTVLFPEGGGQPDDRGTINDISVLRVTRRGEQADHFTQTPLDPGSQVLVRVDWERRFDHMQQHSGQHLITAVADHLFKLKTTSWELGRFRSAIELDTPSMTAEQVAAIEQSVNEKIRDRLPVNVRELSLDDPEVEQVSGRGLPDDHAGPIRVVNIEGVDSNMCCGTHVSNLSDLQVIKILGTEKGKKNRTNLIFLSGNRVLKWMERSHGTEKALTALLKCGAEDHVEAVKKLQNSTKILQKNNLNLLRDLAVHIAHSLRNSPDWGGVVILHRKEGDSEFMNIIANEIGSEETLLFLTVGDEKGGGLFLLAGPPASVETLGPRVAEVLEGKGAGKKGRFQGKATKMSRRMEAQALLQDYISTQSAKE

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Bovine

100

Chicken

Zebrafish

Xenopus

Horse

Sheep

Dog

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9BTE6 As Substrate

Q9BTE6

Site	PTM Type	Source
K34	Ubiquitination	Uniprot
K126	Ubiquitination	Uniprot
K161	Ubiquitination	Uniprot
S174	Phosphorylation	Uniprot
K224	Ubiquitination	Uniprot
K230	Ubiquitination	Uniprot
K248	Ubiquitination	Uniprot
K258	Ubiquitination	Uniprot
K265	Ubiquitination	Uniprot
K277	Ubiquitination	Uniprot
K283	Ubiquitination	Uniprot
K287	Ubiquitination	Uniprot
K376	Ubiquitination	Uniprot

Research Backgrounds

Q9BTE6_AASD1_HUMAN

Function:

Functions in trans to edit the amino acid moiety from incorrectly charged tRNA(Ala).

Subcellular Location:

Cytoplasm.

Family&Domains:

Belongs to the class-II aminoacyl-tRNA synthetase family. Alax-L subfamily.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

AARSD1 Antibody - #DF3751