Product: B3GALT4 Antibody
Catalog: DF3834
Description: Rabbit polyclonal antibody to B3GALT4
Application: WB IF/ICC
Reactivity: Human
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
Mol.Wt.: 42 KD; 42kD(Calculated).
Uniprot: O96024
RRID: AB_2836191

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Prediction:
Pig(83%), Bovine(83%), Horse(92%), Sheep(83%), Rabbit(92%), Dog(92%), Xenopus(83%)
Clonality:
Polyclonal
Specificity:
B3GALT4 Antibody detects endogenous levels of total B3GALT4.
RRID:
AB_2836191
Cite Format: Affinity Biosciences Cat# DF3834, RRID:AB_2836191.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

3-galactosyltransferase 4; 3-galactosyltransferase; 3-GalTase 4; b3Gal-T4; B3GALT4; B3GT4_HUMAN; Beta 1,3 galactosyltransferase 4; Beta 1,3 GalTase 4; Beta 3 galactosyltransferase 4; Beta-1; Beta3Gal-T4; Beta3GalT4; Gal T2; GAL-T2; GALT2; GalT4; Ganglioside galactosyltransferase; OTTHUMP00000029128; UDP Gal:betaGlcNAc beta 1,3 galactosyltransferase, polypeptide 4; UDP galactose:beta N acetyl galactosamine beta 1,3 galactosyltransferase; UDP-galactose:beta-N-acetyl-galactosamine-beta-1;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
O96024 B3GT4_HUMAN:

Highly expressed in heart, skeletal muscle and pancreas and, to a lesser extent, in brain, placenta, kidney, liver and lung.

Sequence:
MQLRLFRRLLLAALLLVIVWTLFGPSGLGEELLSLSLASLLPAPASPGPPLALPRLLIPNQEACSGPGAPPFLLILVCTAPENLNQRNAIRASWGGLREARGLRVQTLFLLGEPNAQHPVWGSQGSDLASESAAQGDILQAAFQDSYRNLTLKTLSGLNWAEKHCPMARYVLKTDDDVYVNVPELVSELVLRGGRWGQWERSTEPQREAEQEGGQVLHSEEVPLLYLGRVHWRVNPSRTPGGRHRVSEEQWPHTWGPFPPYASGTGYVLSASAVQLILKVASRAPLLPLEDVFVGVSARRGGLAPTQCVKLAGATHYPLDRCCYGKFLLTSHRLDPWKMQEAWKLVGGSDGERTAPFCSWFQGVLGILRCRAIAWLQS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
92
Dog
92
Rabbit
92
Pig
83
Bovine
83
Sheep
83
Xenopus
83
Zebrafish
57
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O96024 As Substrate

Site PTM Type Enzyme
S93 Phosphorylation
T330 Phosphorylation

Research Backgrounds

Function:

Involved in GM1/GD1B/GA1 ganglioside biosynthesis.

Subcellular Location:

Golgi apparatus membrane>Single-pass type II membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Highly expressed in heart, skeletal muscle and pancreas and, to a lesser extent, in brain, placenta, kidney, liver and lung.

Family&Domains:

Belongs to the glycosyltransferase 31 family.

Research Fields

· Metabolism > Glycan biosynthesis and metabolism > Glycosphingolipid biosynthesis - ganglio series.

· Metabolism > Global and overview maps > Metabolic pathways.

Restrictive clause

 

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For Research Use Only.
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