CES2 Antibody | Affinity Biosciences

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Western blot analysis of extracts from Jurkat cells, using CES2 antibody.

Western blot analysis of extracts from Mouse brain, using CES2 Antibody.

Product:	CES2 Antibody
Catalog:	DF3895
Description:	Rabbit polyclonal antibody to CES2
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse
Prediction:	Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.:	62 KD; 62kD(Calculated).
Uniprot:	O00748
RRID:	AB_2836248

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Prediction:

Bovine(92%), Horse(92%), Sheep(92%), Rabbit(83%), Dog(83%)

Clonality:

Polyclonal

Specificity:

CES2 Antibody detects endogenous levels of total CES2.

RRID:

AB_2836248
Cite Format: Affinity Biosciences Cat# DF3895, RRID:AB_2836248.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Carboxylesterase 2 (intestine, liver); Carboxylesterase 2; CE-2; CES2; CES2A1; Cocaine esterase; EC 3.1.1.1; EST2_HUMAN; hCE-2; ICE; Iintestinal carboxylesterase liver carboxylesterase 2; Intestinal carboxylesterase; liver carboxylesterase-2; Methylumbelliferyl acetate deacetylase 2; PCE 2; PCE-2;

Immunogens

Immunogen:

Uniprot:

O00748(EST2_HUMAN) >>Visit HPA database.

Gene(ID):

CES2(8824)

Expression:

O00748 EST2_HUMAN:

Preferentially expressed in intestine with moderate expression in liver. Within the intestine, highest expression is found in small intestine with lower expression in colon and rectum.

Sequence:

O00748 EST2_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MRLHRLRARLSAVACGLLLLLVRGQGQDSASPIRTTHTGQVLGSLVHVKGANAGVQTFLGIPFAKPPLGPLRFAPPEPPESWSGVRDGTTHPAMCLQDLTAVESEFLSQFNMTFPSDSMSEDCLYLSIYTPAHSHEGSNLPVMVWIHGGALVFGMASLYDGSMLAALENVVVVIIQYRLGVLGFFSTGDKHATGNWGYLDQVAALRWVQQNIAHFGGNPDRVTIFGESAGGTSVSSLVVSPISQGLFHGAIMESGVALLPGLIASSADVISTVVANLSACDQVDSEALVGCLRGKSKEEILAINKPFKMIPGVVDGVFLPRHPQELLASADFQPVPSIVGVNNNEFGWLIPKVMRIYDTQKEMDREASQAALQKMLTLLMLPPTFGDLLREEYIGDNGDPQTLQAQFQEMMADSMFVIPALQVAHFQCSRAPVYFYEFQHQPSWLKNIRPPHMKADHGDELPFVFRSFFGGNYIKFTEEEEQLSRKMMKYWANFARNGNPNGEGLPHWPLFDQEEQYLQLNLQPAVGRALKAHRLQFWKKALPQKIQELEEPEERHTEL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Horse

Bovine

Sheep

Dog

Rabbit

Zebrafish

Pig

Xenopus

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O00748 As Substrate

O00748

Site	PTM Type	Source
T38	Phosphorylation	Uniprot
S44	Phosphorylation	Uniprot
N111	N-Glycosylation	Uniprot
S368	Phosphorylation	Uniprot
K489	Acetylation	Uniprot
K545	Ubiquitination	Uniprot

Research Backgrounds

O00748_EST2_HUMAN

Function:

Involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs. Shows high catalytic efficiency for hydrolysis of cocaine, 4-methylumbelliferyl acetate, heroin and 6-monoacetylmorphine. Hydrolyzes aspirin, substrates with large alcohol group and small acyl group and endogenous lipids such as triacylglycerol.

PTMs:

Glycosylated.

Subcellular Location:

Endoplasmic reticulum lumen.

Tissue Specificity:

Preferentially expressed in intestine with moderate expression in liver. Within the intestine, highest expression is found in small intestine with lower expression in colon and rectum.

Subunit Structure:

Monomer.

Family&Domains:

Belongs to the type-B carboxylesterase/lipase family.

Research Fields

· Metabolism > Xenobiotics biodegradation and metabolism > Drug metabolism - other enzymes.

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CES2 Antibody - #DF3895