CLN5 Antibody | Affinity Biosciences

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Western blot analysis of extracts from mouse brain, using CLN5 antibody.

Western blot analysis of extracts from Rat brain, using CLN5 Antibody.

Product:	CLN5 Antibody
Catalog:	DF3927
Description:	Rabbit polyclonal antibody to CLN5
Application:	WB IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Sheep, Rabbit, Dog, Chicken
Mol.Wt.:	46 KD; 41kD(Calculated).
Uniprot:	O75503
RRID:	AB_2836280

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(91%), Bovine(100%), Sheep(100%), Rabbit(91%), Dog(91%), Chicken(91%)

Clonality:

Polyclonal

Specificity:

CLN5 Antibody detects endogenous levels of total CLN5.

RRID:

AB_2836280
Cite Format: Affinity Biosciences Cat# DF3927, RRID:AB_2836280.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Ceroid lipofuscinosis neuronal 5; Ceroid-lipofuscinosis neuronal protein 5; CLN5; CLN5_HUMAN; NCL; Protein CLN5;

Immunogens

Immunogen:

Uniprot:

O75503(CLN5_HUMAN) >>Visit HPA database.

Gene(ID):

CLN5(1203)

Expression:

O75503 CLN5_HUMAN:

Ubiquitous.

Sequence:

O75503 CLN5_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAQEVDTAQGAEMRRGAGAARGRASWCWALALLWLAVVPGWSRVSGIPSRRHWPVPYKRFDFRPKPDPYCQAKYTFCPTGSPIPVMEGDDDIEVFRLQAPVWEFKYGDLLGHLKIMHDAIGFRSTLTGKNYTMEWYELFQLGNCTFPHLRPEMDAPFWCNQGAACFFEGIDDVHWKENGTLVQVATISGNMFNQMAKWVKQDNETGIYYETWNVKASPEKGAETWFDSYDCSKFVLRTFNKLAEFGAEFKNIETNYTRIFLYSGEPTYLGNETSVFGPTGNKTLGLAIKRFYYPFKPHLPTKEFLLSLLQIFDAVIVHKQFYLFYNFEYWFLPMKFPFIKITYEEIPLPIRNKTLSGL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Bovine

100

Sheep

100

Pig

Dog

Chicken

Rabbit

Zebrafish

Horse

Xenopus

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O75503 As Substrate

O75503

Site	PTM Type	Source
K65	Ubiquitination	Uniprot
K105	Ubiquitination	Uniprot
N130	N-Glycosylation	Uniprot
N143	N-Glycosylation	Uniprot
N178	N-Glycosylation	Uniprot
N203	N-Glycosylation	Uniprot
K215	Ubiquitination	Uniprot
N255	N-Glycosylation	Uniprot
N271	N-Glycosylation	Uniprot
N281	N-Glycosylation	Uniprot
T283	Phosphorylation	Uniprot
N352	N-Glycosylation	Uniprot

Research Backgrounds

O75503_CLN5_HUMAN

Function:

Plays a role in influencing the retrograde trafficking of lysosomal sorting receptors SORT1 and IGF2R from the endosomes to the trans-Golgi network by controlling the recruitment of retromer complex to the endosomal membrane. Regulates the localization and activation of RAB7A which is required to recruit the retromer complex to the endosomal membrane.

PTMs:

N-glycosylated with both high mannose and complex type sugars. Glycosylation is important for proper folding and trafficking to the lysosomes.

The type II membrane signal anchor is proteolytically cleaved to produce a mature form that is transported to the lysosomes (Ceroid-lipofuscinosis neuronal protein 5, secreted form).

Can undergo proteolytic cleavage at the C-terminus, probably by a cysteine protease and may involve the removal of approximately 10-15 residues from the C-terminal end.

Subcellular Location:

Lysosome.

Membrane>Single-pass type II membrane protein.
Note: An amphipathic anchor region facilitates its association with the membrane.

Tissue Specificity:

Ubiquitous.

Subunit Structure:

Interacts with SORT1, RAB5A and RAB7A. Interacts with PPT1, TPP1, CLN3, CLN6, CLN8, ATP5F1A and ATP5F1B (By similarity).

Family&Domains:

Belongs to the CLN5 family.

Research Fields

· Cellular Processes > Transport and catabolism > Lysosome. (View pathway)

Restrictive clause

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CLN5 Antibody - #DF3927