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$280 100ul
$350 200ul

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  • Product Name
    NT5C1A Antibody
  • Catalog No.
    DF3978
  • RRID
    AB_2836338
  • Source
    Rabbit
  • Application
    WB,IF/ICC,ELISA(peptide)
  • Reactivity
    Human, Mouse
  • Prediction
    Pig, Bovine, Horse, Sheep, Rabbit, Dog
  • UniProt
  • Mol.Wt
    41 kD;
    41kDa(Calculated).
  • Concentration
    1mg/ml
  • Browse similar products>>

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Product Information

Alternative Names:Expand▼

5' nucleotidase cytosolic IA; 5NT1A_HUMAN; AMP specific 5' NT; CN I; CN IA; cN-I; cN-IA; CN1; cN1A; cNI; cNIA; Cytosolic 5' nucleotidase 1A; Cytosolic 5' nucleotidase IA; Cytosolic 5' nucleotidase type 1A; Cytosolic 5''-nucleotidase 1A; Cytosolic 5''-nucleotidase IA; MGC119199; MGC119201; NT5C1A; nucleotidase, 5-prime, cytosolic, IA;

Applications:

WB 1:500-1:1000, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000
*The optimal dilutions should be determined by the end user.

Reactivity:

Human, Mouse

Predicted Reactivity:

Pig, Bovine, Horse, Sheep, Rabbit, Dog

Source:

Rabbit

Clonality:

Polyclonal

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Specificity:

NT5C1A Antibody detects endogenous levels of total NT5C1A.

RRID:

AB_2836338
Please cite this product as: Affinity Biosciences Cat# DF3978, RRID:AB_2836338.

Format:

Liquid

Concentration:

1mg/ml

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

Immunogen Information in 3D

Immunogen:

A synthesized peptide derived from human NT5C1A, corresponding to a region within the internal amino acids.

Uniprot:



>>Visit The Human Protein Atlas

Gene ID:

Gene Name:

NT5C1A

Molecular Weight:

Observed Mol.Wt.: 41 kD.
Predicted Mol.Wt.: 41kDa(Calculated)..

Subcellular Location:

Cytoplasm.

Tissue Specificity:

Q9BXI3 5NT1A_HUMAN:
Highly expressed in skeletal muscle. Detected at intermediate levels in heart, brain, kidney and pancreas.

Sequence:
MEPGQPREPQEPREPGPGAETAAAPVWEEAKIFYDNLAPKKKPKSPKPQNAVTIAVSSRALFRMDEEQQIYTEQGVEEYVRYQLEHENEPFSPGPAFPFVKALEAVNRRLRELYPDSEDVFDIVLMTNNHAQVGVRLINSINHYDLFIERFCMTGGNSPICYLKAYHTNLYLSADAEKVREAIDEGIAAATIFSPSRDVVVSQSQLRVAFDGDAVLFSDESERIVKAHGLDRFFEHEKAHENKPLAQGPLKGFLEALGRLQKKFYSKGLRLECPIRTYLVTARSAASSGARALKTLRSWGLETDEALFLAGAPKGPLLEKIRPHIFFDDQMFHVAGAQEMGTVAAHVPYGVAQTPRRTAPAKQAPSAQ

Research Background

Function:

Dephosphorylates the 5' and 2'(3')-phosphates of deoxyribonucleotides and has a broad substrate specificity. Helps to regulate adenosine levels in heart during ischemia and hypoxia.

Subcellular Location:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte

Tissue Specificity:

Highly expressed in skeletal muscle. Detected at intermediate levels in heart, brain, kidney and pancreas.

Similarity:

Belongs to the 5'-nucleotidase type 3 family.

Research Fields

Research Fields:

· Metabolism > Nucleotide metabolism > Purine metabolism.
· Metabolism > Nucleotide metabolism > Pyrimidine metabolism.
· Metabolism > Metabolism of cofactors and vitamins > Nicotinate and nicotinamide metabolism.
· Metabolism > Global and overview maps > Metabolic pathways.

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Catalog Number :

DF3978-BP
(Blocking peptide available as DF3978-BP)

Price/Size :

$350/1mg.
Tips: For modified antibodies, we provide modified peptides(0.5mg) and non-modified peptides(0.5mg).

Function :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

Format and storage :

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.

Precautions :

This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Horse
100%
Dog
100%
Pig
91%
Bovine
91%
Sheep
91%
Rabbit
91%
Chicken
73%
Xenopus
0%
Zebrafish
0%
High similarity Medium similarity Low similarity No similarity
Q9BXI3 as Substrate
Site PTM Type Enzyme
K31 Acetylation
Y34 Phosphorylation
S57 Phosphorylation
S58 Phosphorylation
Y79 Phosphorylation
Y265 Phosphorylation
S266 Phosphorylation
K314 Acetylation
IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.

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