DNTT Antibody | Affinity Biosciences

WB
IF/ICC

1/2

Western blot analysis of extracts from Mouse testis, using DNTT Antibody at 1/1000 dilution.

Product:	DNTT Antibody
Catalog:	DF3996
Description:	Rabbit polyclonal antibody to DNTT
Application:	WB IF/ICC
Reactivity:	Human
Prediction:	Mouse
Mol.Wt.:	58 KD; 59kD(Calculated).
Uniprot:	P04053
RRID:	AB_2836356

View similar products>>

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors

Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Prediction:

Mouse(%)

Clonality:

Polyclonal

Specificity:

DNTT Antibody detects endogenous levels of total DNTT.

RRID:

AB_2836356
Cite Format: Affinity Biosciences Cat# DF3996, RRID:AB_2836356.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Deoxynucleotidyltransferase terminal; DNA nucleotidylexotransferase; DNTT; Nucleosidetriphosphate DNA deoxynucleotidylexotransferase; TDT; TDT_HUMAN; Terminal addition enzyme; Terminal deoxynucleotidyltransferase; Terminal deoxyribonucleotidyltransferase; Terminal transferase;

Immunogens

Immunogen:

Uniprot:

P04053(TDT_HUMAN) >>Visit HPA database.

Gene(ID):

DNTT(1791)

Sequence:

P04053 TDT_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MDPPRASHLSPRKKRPRQTGALMASSPQDIKFQDLVVFILEKKMGTTRRAFLMELARRKGFRVENELSDSVTHIVAENNSGSDVLEWLQAQKVQVSSQPELLDVSWLIECIRAGKPVEMTGKHQLVVRRDYSDSTNPGPPKTPPIAVQKISQYACQRRTTLNNCNQIFTDAFDILAENCEFRENEDSCVTFMRAASVLKSLPFTIISMKDTEGIPCLGSKVKGIIEEIIEDGESSEVKAVLNDERYQSFKLFTSVFGVGLKTSEKWFRMGFRTLSKVRSDKSLKFTRMQKAGFLYYEDLVSCVTRAEAEAVSVLVKEAVWAFLPDAFVTMTGGFRRGKKMGHDVDFLITSPGSTEDEEQLLQKVMNLWEKKGLLLYYDLVESTFEKLRLPSRKVDALDHFQKCFLIFKLPRQRVDSDQSSWQEGKTWKAIRVDLVLCPYERRAFALLGWTGSRQFERDLRRYATHERKMILDNHALYDKTKRIFLKAESEEEIFAHLGLDYIEPWERNA

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Rabbit

Pig

Horse

Bovine

Sheep

Dog

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P04053 As Substrate

P04053

Site	PTM Type	Source
S10	Phosphorylation	Uniprot
K122	Ubiquitination	Uniprot
S132	Phosphorylation	Uniprot
T135	Phosphorylation	Uniprot
T142	Phosphorylation	Uniprot
K149	Ubiquitination	Uniprot
K199	Ubiquitination	Uniprot
K209	Ubiquitination	Uniprot
K220	Ubiquitination	Uniprot
K222	Ubiquitination	Uniprot
K238	Ubiquitination	Uniprot
K250	Ubiquitination	Uniprot
K261	Ubiquitination	Uniprot
K265	Ubiquitination	Uniprot
K290	Ubiquitination	Uniprot
K316	Acetylation	Uniprot
K339	Ubiquitination	Uniprot
K363	Ubiquitination	Uniprot
K370	Ubiquitination	Uniprot
K371	Ubiquitination	Uniprot
S382	Phosphorylation	Uniprot
T383	Phosphorylation	Uniprot
K393	Ubiquitination	Uniprot
K402	Ubiquitination	Uniprot
K408	Ubiquitination	Uniprot
K425	Ubiquitination	Uniprot
K468	Ubiquitination	Uniprot
Y477	Phosphorylation	Uniprot
K479	Ubiquitination	Uniprot

Research Backgrounds

P04053_TDT_HUMAN

Function:

Template-independent DNA polymerase which catalyzes the random addition of deoxynucleoside 5'-triphosphate to the 3'-end of a DNA initiator. One of the in vivo functions of this enzyme is the addition of nucleotides at the junction (N region) of rearranged Ig heavy chain and T-cell receptor gene segments during the maturation of B- and T-cells.

Subcellular Location:

Nucleus.

Subunit Structure:

Interacts with PRP19 and DNTTIP1. Forms a ternary complex with DNTTIP2 and core histone. Released from this complex by PCNA. Interacts with TRERF1.

Family&Domains:

Belongs to the DNA polymerase type-X family.

Research Fields

· Genetic Information Processing > Replication and repair > Non-homologous end-joining.

· Organismal Systems > Immune system > Hematopoietic cell lineage. (View pathway)

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

DNTT Antibody - #DF3996