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Product Info

Source:
Mouse
Application:
ELISA 1:10000, WB 1:500-1:2000, IHC 1:200-1:1000
*The optimal dilutions should be determined by the end user.
Reactivity:
Human
Clonality:
Monoclonal [AFB1554]
Specificity:
CK1 antibody detects endogenous levels of total CK1.
RRID:
AB_2833579
Cite Format: Affinity Biosciences Cat# BF0141, RRID:AB_2833579.
Purification:
Affinity-chromatography.
Storage:
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Casein kinase 1 alpha 1; Casein kinase I isoform alpha; CK1; CK1A; CKI alpha; CKI-alpha; CKIa; Clock regulator kinase; Csnk1a1; Down regulated in lung cancer; HLCDGP1; KC1A_HUMAN; PRO2975;

Immunogens

Immunogen:

Purified recombinant fragment of human CK1 expressed in E. Coli.

Uniprot:
Gene(ID):
Description:
CK1 (also designated Cytokeratin 1 or KRT1 ),with 644-amino acid protein (about 70kDa), is a member of the keratin family. Cytokeratins play a critical role in differentiation and tissue specialization, and they function to maintain the overall structural integrity of epithelial cells.CK1 consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. CK1 is specifically expressed in the spinous and granular layers of the epidermis with family member KRT10 and mutations in these genes have been associated with bullous congenital ichthyosiform erythroderma.
Sequence:
MASSSGSKAEFIVGGKYKLVRKIGSGSFGDIYLAINITNGEEVAVKLESQKARHPQLLYESKLYKILQGGVGIPHIRWYGQEKDYNVLVMDLLGPSLEDLFNFCSRRFTMKTVLMLADQMISRIEYVHTKNFIHRDIKPDNFLMGIGRHCNKLFLIDFGLAKKYRDNRTRQHIPYREDKNLTGTARYASINAHLGIEQSRRDDMESLGYVLMYFNRTSLPWQGLKAATKKQKYEKISEKKMSTPVEVLCKGFPAEFAMYLNYCRGLRFEEAPDYMYLRQLFRILFRTLNHQYDYTFDWTMLKQKAAQQAASSSGQGQQAQTPTGKQTDKTKSNMKGF

PTMs - P48729 As Substrate

Site PTM Type Enzyme
A2 Acetylation
S3 Phosphorylation
S4 Phosphorylation
K8 Acetylation
K8 Ubiquitination
K16 Acetylation
K16 Ubiquitination
Y17 Phosphorylation
Y32 Phosphorylation
K51 Ubiquitination
Y59 Phosphorylation
K62 Ubiquitination
K65 Ubiquitination
Y85 Phosphorylation
S105 Phosphorylation
K138 Ubiquitination
K152 Ubiquitination
K162 Ubiquitination
K179 Ubiquitination
T184 Phosphorylation
S199 Phosphorylation
S218 Phosphorylation P17612 (PRKACA)
K225 Ubiquitination
K229 Ubiquitination
K240 Ubiquitination
S242 Phosphorylation P17612 (PRKACA)
Y274 Phosphorylation
T287 Phosphorylation
Y292 Phosphorylation
Y294 Phosphorylation
K302 Ubiquitination
K304 Ubiquitination
S311 Phosphorylation
S313 Phosphorylation
T321 Phosphorylation
T323 Phosphorylation
K325 Ubiquitination
T327 Phosphorylation
S332 Phosphorylation
K335 Ubiquitination

PTMs - P48729 As Enzyme

Substrate Site Source
O15151 (MDM4) S289 Uniprot
O43524 (FOXO3) S318 Uniprot
O43524 (FOXO3) S321 Uniprot
O60260 (PRKN) S101 Uniprot
O60260 (PRKN) S378 Uniprot
O60346 (PHLPP1) S1359 Uniprot
O60346 (PHLPP1) T1363 Uniprot
O60346 (PHLPP1) S1379 Uniprot
O60346 (PHLPP1) S1381 Uniprot
O75581 (LRP6) T1493 Uniprot
O94916 (NFAT5) S158 Uniprot
P01106 (MYC) S252 Uniprot
P02730 (SLC4A1) T42 Uniprot
P02730 (SLC4A1) S303 Uniprot
P04637-1 (TP53) S6 Uniprot
P04637-1 (TP53) S9 Uniprot
P04637 (TP53) T18 Uniprot
P04637 (TP53) S20 Uniprot
P07910-2 (HNRNPC) S240 Uniprot
P07910-1 (HNRNPC) S253 Uniprot
P07910 (HNRNPC) S260 Uniprot
P07910 (HNRNPC) S299 Uniprot
P11308 (ERG) S44 Uniprot
P11308 (ERG) S45 Uniprot
P11308 (ERG) S46 Uniprot
P12830 (CDH1) S844 Uniprot
P16220 (CREB1) S108 Uniprot
P16220 (CREB1) S111 Uniprot
P16220 (CREB1) S114 Uniprot
P16220 (CREB1) S156 Uniprot
P17181 (IFNAR1) S535 Uniprot
P17931 (LGALS3) S6 Uniprot
P17931 (LGALS3) S12 Uniprot
P18846 (ATF1) S36 Uniprot
P18846 (ATF1) S41 Uniprot
P18846 (ATF1) S47 Uniprot
P18846 (ATF1) S50 Uniprot
P18846 (ATF1) S51 Uniprot
P25054 (APC) S1504 Uniprot
P25054 (APC) S1505 Uniprot
P25054 (APC) S1507 Uniprot
P25054 (APC) S1510 Uniprot
P27348 (YWHAQ) S232 Uniprot
P30304 (CDC25A) S79 Uniprot
P30304 (CDC25A) S82 Uniprot
P35222 (CTNNB1) S45 Uniprot
P37840 (SNCA) S87 Uniprot
P37840 (SNCA) S129 Uniprot
P40337 (VHL) S72 Uniprot
P42892 (ECE1) S34 Uniprot
P42892 (ECE1) S36 Uniprot
P49810-1 (PSEN2) S7 Uniprot
P49810-1 (PSEN2) S9 Uniprot
P49810-3 (PSEN2) S19 Uniprot
P55316 (FOXG1) S19 Uniprot
P55957-1 (BID) T59 Uniprot
P55957 (BID) S64 Uniprot
P56537 (EIF6) S174 Uniprot
P56537 (EIF6) S175 Uniprot
P56817 (BACE1) S498 Uniprot
P62745 (RHOB) S185 Uniprot
P63104-1 (YWHAZ) T232 Uniprot
Q00535 (CDK5) S159 Uniprot
Q03060-12 (CREM) S36 Uniprot
Q03060-30 (CREM) S50 Uniprot
Q03060-32 (CREM) S66 Uniprot
Q03060-16 (CREM) S110 Uniprot
Q04206 (RELA) S316 Uniprot
Q05940-1 (SLC18A2) S511 Uniprot
Q12778 (FOXO1) S322 Uniprot
Q12778 (FOXO1) S325 Uniprot
Q12968-2 (NFATC3) T204 Uniprot
Q12968-1 (NFATC3) S207 Uniprot
Q12968-2 (NFATC3) T210 Uniprot
Q12968-1 (NFATC3) S211 Uniprot
Q12968-3 (NFATC3) S215 Uniprot
Q13144 (EIF2B5) S51 Uniprot
Q13144 (EIF2B5) S466 Uniprot
Q13144 (EIF2B5) S469 Uniprot
Q13148 (TARDBP) S2 Uniprot
Q13148 (TARDBP) Y4 Uniprot
Q13148 (TARDBP) T25 Uniprot
Q13148 (TARDBP) T88 Uniprot
Q13148 (TARDBP) S91 Uniprot
Q13148 (TARDBP) S92 Uniprot
Q13148 (TARDBP) T116 Uniprot
Q13148 (TARDBP) S183 Uniprot
Q13148 (TARDBP) S242 Uniprot
Q13148 (TARDBP) S254 Uniprot
Q13148 (TARDBP) S273 Uniprot
Q13148 (TARDBP) S292 Uniprot
Q13148 (TARDBP) S305 Uniprot
Q13148 (TARDBP) S342 Uniprot
Q13148 (TARDBP) S347 Uniprot
Q13148 (TARDBP) S350 Uniprot
Q13148 (TARDBP) S369 Uniprot
Q13148 (TARDBP) S375 Uniprot
Q13148 (TARDBP) S377 Uniprot
Q13148 (TARDBP) S379 Uniprot
Q13148 (TARDBP) S387 Uniprot
Q13148 (TARDBP) S389 Uniprot
Q13148 (TARDBP) S393 Uniprot
Q13148 (TARDBP) S395 Uniprot
Q13148 (TARDBP) S403 Uniprot
Q13148 (TARDBP) S404 Uniprot
Q13148 (TARDBP) S407 Uniprot
Q13148 (TARDBP) S409 Uniprot
Q13148 (TARDBP) S410 Uniprot
Q13158 (FADD) S194 Uniprot
Q6IE81 (JADE1) S18 Uniprot
Q6IE81 (JADE1) S20 Uniprot
Q8TB45 (DEPTOR) T149 Uniprot
Q8TB45 (DEPTOR) S263 Uniprot
Q8TB45 (DEPTOR) S286 Uniprot
Q8TB45 (DEPTOR) S287 Uniprot
Q8TB45 (DEPTOR) S291 Uniprot
Q9BXL7 (CARD11) S615 Uniprot
Q9H6E5 (TUT1) S6 Uniprot
Q9NRF8 (CTPS2) S568 Uniprot
Q9Y2W7 (KCNIP3) S63 Uniprot
Q9Y4G8 (RAPGEF2) S1244 Uniprot
Q9Y4G8 (RAPGEF2) S1248 Uniprot

Research Backgrounds

Function:

Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling. Phosphorylates CTNNB1 at 'Ser-45'. May phosphorylate PER1 and PER2. May play a role in segregating chromosomes during mitosis. May play a role in keratin cytoskeleton disassembly and thereby, it may regulate epithelial cell migration.

Subcellular Location:

Cytoplasm. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome. Chromosome>Centromere>Kinetochore. Nucleus speckle. Cytoplasm>Cytoskeleton>Cilium basal body.
Note: Localizes to the centrosome in interphase cells, and to kinetochore fibers during mitosis. Also recruited to the keratin cytoskeleton (PubMed:23902688).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Monomer. Interacts with the Axin complex. Interacts with TUT1, leading to TUT1 phosphorylation. Interacts with FAM83H; recruits CSNK1A1 to keratin filaments.

Family&Domains:

Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. Casein kinase I subfamily.

Research Fields

· Environmental Information Processing > Signal transduction > Wnt signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Hedgehog signaling pathway.   (View pathway)

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Human Diseases > Cancers: Specific types > Breast cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Hepatocellular carcinoma.   (View pathway)

· Human Diseases > Cancers: Specific types > Gastric cancer.   (View pathway)

References

1). Wang X et al. Effects of endometrial stem cell transplantation combined with estrogen in the repair of endometrial injury. Oncol Lett 2018 Jul;16(1):1115-1122 (PubMed: 29963188)

Application: WB    Species: rat    Sample: EnSCs

Figure 2. |Expression of EMA, CK, CD49f, THY‑1, Col I. 5B5 and vimentin in EnSCs. (A) mRNA levels of EMA, CK and CD49f in EnSCs. (B) mRNA levels of THY‑1, Col I, 5B5 and vimentin in EnSCs. (C) Protein expression of EMA, CK and CD49f in EnSCs.

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