Product: TUBGCP4 Antibody
Catalog: DF4075
Description: Rabbit polyclonal antibody to TUBGCP4
Application: WB IF/ICC
Reactivity: Human, Mouse
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 76 KD; 76kD(Calculated).
Uniprot: Q9UGJ1
RRID: AB_2836449

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(80%), Dog(90%)
Clonality:
Polyclonal
Specificity:
TUBGCP4 Antibody detects endogenous levels of total TUBGCP4.
RRID:
AB_2836449
Cite Format: Affinity Biosciences Cat# DF4075, RRID:AB_2836449.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

76P; FLJ14797; Gamma tubulin complex component 4; Gamma tubulin ring complex protein (76p gene); Gamma tubulin ring complex protein; Gamma-ring complex protein 76 kDa; GCP 4; GCP4; Grip76; h76p; hGCP4; Hgrip76; TUBGCP 4; TUBGCP4; Tubulin gamma complex associated protein 4;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
Q9UGJ1 GCP4_HUMAN:

Ubiquitously expressed.

Sequence:
MIHELLLALSGYPGSIFTWNKRSGLQVSQDFPFLHPSETSVLNRLCRLGTDYIRFTEFIEQYTGHVQQQDHHPSQQGQGGLHGIYLRAFCTGLDSVLQPYRQALLDLEQEFLGDPHLSISHVNYFLDQFQLLFPSVMVVVEQIKSQKIHGCQILETVYKHSCGGLPPVRSALEKILAVCHGVMYKQLSAWMLHGLLLDQHEEFFIKQGPSSGNVSAQPEEDEEDLGIGGLTGKQLRELQDLRLIEEENMLAPSLKQFSLRVEILPSYIPVRVAEKILFVGESVQMFENQNVNLTRKGSILKNQEDTFAAELHRLKQQPLFSLVDFEQVVDRIRSTVAEHLWKLMVEESDLLGQLKIIKDFYLLGRGELFQAFIDTAQHMLKTPPTAVTEHDVNVAFQQSAHKVLLDDDNLLPLLHLTIEYHGKEHKADATQAREGPSRETSPREAPASGWAALGLSYKVQWPLHILFTPAVLEKYNVVFKYLLSVRRVQAELQHCWALQMQRKHLKSNQTDAIKWRLRNHMAFLVDNLQYYLQVDVLESQFSQLLHQINSTRDFESIRLAHDHFLSNLLAQSFILLKPVFHCLNEILDLCHSFCSLVSQNLGPLDERGAAQLSILVKGFSRQSSLLFKILSSVRNHQINSDLAQLLLRLDYNKYYTQAGGTLGSFGM

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
90
Rabbit
80
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9UGJ1 As Substrate

Site PTM Type Enzyme
K21 Methylation
K147 Ubiquitination
K233 Ubiquitination
K255 Ubiquitination
S298 Phosphorylation
K301 Ubiquitination
K315 Ubiquitination
K355 Ubiquitination
K358 Ubiquitination
T375 Phosphorylation
T385 Phosphorylation
S441 Phosphorylation
K506 Ubiquitination
S624 Phosphorylation

Research Backgrounds

Function:

Gamma-tubulin complex is necessary for microtubule nucleation at the centrosome.

Subcellular Location:

Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitously expressed.

Subunit Structure:

Gamma-tubulin complex is composed of gamma-tubulin, TUBGCP2, TUBGCP3, TUBGCP4, TUBGCP5 and TUBGCP6. Interacts with NINL. Interacts with ATF5; the ATF5:PCNT:polyglutamylated tubulin (PGT) tripartite unites the mother centriole and the pericentriolar material (PCM) in the centrosome.

Family&Domains:

Belongs to the TUBGCP family.

Restrictive clause

 

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