CEP97 Antibody - #DF4137

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Product: CEP97 Antibody
Catalog: DF4137
Description: Rabbit polyclonal antibody to CEP97
Application: WB IF/ICC
Reactivity: Human, Mouse
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 97 KD; 97kD(Calculated).
Uniprot: Q8IW35
RRID: AB_2836502

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(100%), Bovine(91%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
CEP97 Antibody detects endogenous levels of total CEP97.
RRID:
AB_2836502
Cite Format: Affinity Biosciences Cat# DF4137, RRID:AB_2836502.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

2810403B08Rik; 4932439K18Rik; AA437768; Centrosomal protein 97kDa; Centrosomal protein of 97 kDa; CEP 97; Cep97; CEP97_HUMAN; E130116N02Rik; FLJ23047; FLJ26462; Leucine rich repeat and IQ domain containing protein 2; Leucine rich repeats and IQ motif containing 2; Leucine-rich repeat and IQ domain-containing protein 2; LRRIQ2; RGD1307400;

Immunogens

Immunogen:
Uniprot:

Q8IW35(CEP97_HUMAN) >>Visit HPA database.

Gene(ID):
CEP97(79598)
Sequence:
MAVARVDAALPPGEGSVVNWSGQGLQKLGPNLPCEADIHTLILDKNQIIKLENLEKCKRLIQLSVANNRLVRMMGVAKLTLLRVLNLPHNSIGCVEGLKELVHLEWLNLAGNNLKAMEQINSCTALQHLDLSDNNISQIGDLSKLVSLKTLLLHGNIITSLRMAPAYLPRSLAILSLAENEIRDLNEISFLASLTELEQLSIMNNPCVMATPSIPGFDYRPYIVSWCLNLRVLDGYVISQKESLKAEWLYSQGKGRAYRPGQHIQLVQYLATVCPLTSTLGLQTAEDAKLEKILSKQRFHQRQLMNQSQNEELSPLVPVETRASLIPEHSSPVQDCQISQESEPVIQVNSWVGINSNDDQLFAVKNNFPASVHTTRYSRNDLHLEDIQTDEDKLNCSLLSSESTFMPVASGLSPLSPTVELRLQGINLGLEDDGVADESVKGLESQVLDKEEEQPLWAANENSVQMMRSEINTEVNEKAGLLPCPEPTIISAILKDDNHSLTFFPESTEQKQSDIKKPENTQPENKETISQATSEKLPMILTQRSVALGQDKVALQKLNDAATKLQACWRGFYARNYNPQAKDVRYEIRLRRMQEHIVCLTDEIRRLRKERDEERIKKFVQEEAFRFLWNQVRSLQVWQQTVDQRLSSWHTDVPPISSTLVPSKHPLFTQSQESSCDQNADWFIASDVAPQEKSLPEFPDSGFHSSLTEQVHSLQHSLDFEKSSTEGSESSIMGNSIDTVRYGKESDLGDVSEEHGEWNKESSNNEQDNSLLEQYLTSVQQLEDADERTNFDTETRDSKLHIACFPVQLDTLSDGASVDESHGISPPLQGEISQTQENSKLNAEVQGQQPECDSTFQLLHVGVTV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Sheep
100
Dog
100
Rabbit
100
Bovine
91
Xenopus
70
Chicken
55
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q8IW35 As Substrate

Site PTM Type Enzyme
K27 Ubiquitination
K45 Ubiquitination
K50 Ubiquitination
S64 Phosphorylation
K144 Ubiquitination
K241 Ubiquitination
K245 Ubiquitination
K254 Ubiquitination
K292 Ubiquitination
S308 Phosphorylation
S314 Phosphorylation
S371 Phosphorylation
S378 Phosphorylation
S403 Phosphorylation
S413 Phosphorylation
S416 Phosphorylation
T418 Phosphorylation
S439 Phosphorylation
K478 Ubiquitination
K495 Ubiquitination
S500 Phosphorylation
K511 Ubiquitination
T542 Phosphorylation
S545 Phosphorylation
K552 Ubiquitination
K557 Ubiquitination
K564 Ubiquitination
K582 Ubiquitination
K618 Ubiquitination
S694 Phosphorylation
T708 Phosphorylation
S717 Phosphorylation
S728 Phosphorylation
S736 Phosphorylation
K744 Ubiquitination
S746 Phosphorylation
S752 Phosphorylation
S763 Phosphorylation
S770 Phosphorylation
T793 Phosphorylation
K799 Ubiquitination
T811 Phosphorylation
S813 Phosphorylation
S817 Phosphorylation
S821 Phosphorylation
S825 Phosphorylation
S833 Phosphorylation

Research Backgrounds

Function:

Acts as a key negative regulator of ciliogenesis in collaboration with CCP110 by capping the mother centriole thereby preventing cilia formation. Required for recruitment of CCP110 to the centrosome.

Subcellular Location:

Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Interacts with CALM1, CEP76, CCP110, KIF24, TALPID3. Via its interaction with CCP110, may indirectly interact with HERC2 and NEURL4.

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