NOC2L Antibody - #DF4262

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Product: NOC2L Antibody
Catalog: DF4262
Description: Rabbit polyclonal antibody to NOC2L
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 85 KD; 85kD(Calculated).
Uniprot: Q9Y3T9
RRID: AB_2836613

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(91%)
Clonality:
Polyclonal
Specificity:
NOC2L Antibody detects endogenous levels of total NOC2L.
RRID:
AB_2836613
Cite Format: Affinity Biosciences Cat# DF4262, RRID:AB_2836613.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

NET15; NET7; NIR; NOC2 like; NOC2 like nucleolar associated transcriptional repressor; NOC2-like protein; NOC2L; NOC2L nucleolar associated transcriptional repressor; NOC2L_HUMAN; Novel INHAT (inhibitor of histone acetyltransferase) repressor; Novel INHAT repressor; Nucleolar complex associated 2 homolog (S. cerevisiae); Nucleolar complex protein 2 homolog; PPP1R112; Protein NOC2 homolog; Protein phosphatase 1 regulatory subunit 12;

Immunogens

Immunogen:
Uniprot:

Q9Y3T9(NOC2L_HUMAN) >>Visit HPA database.

Gene(ID):
NOC2L(26155)
Sequence:
MAAAGSRKRRLAELTVDEFLASGFDSESESESENSPQAETREAREAARSPDKPGGSPSASRRKGRASEHKDQLSRLKDRDPEFYKFLQENDQSLLNFSDSDSSEEEEGPFHSLPDVLEEASEEEDGAEEGEDGDRVPRGLKGKKNSVPVTVAMVERWKQAAKQRLTPKLFHEVVQAFRAAVATTRGDQESAEANKFQVTDSAAFNALVTFCIRDLIGCLQKLLFGKVAKDSSRMLQPSSSPLWGKLRVDIKAYLGSAIQLVSCLSETTVLAAVLRHISVLVPCFLTFPKQCRMLLKRMVIVWSTGEESLRVLAFLVLSRVCRHKKDTFLGPVLKQMYITYVRNCKFTSPGALPFISFMQWTLTELLALEPGVAYQHAFLYIRQLAIHLRNAMTTRKKETYQSVYNWQYVHCLFLWCRVLSTAGPSEALQPLVYPLAQVIIGCIKLIPTARFYPLRMHCIRALTLLSGSSGAFIPVLPFILEMFQQVDFNRKPGRMSSKPINFSVILKLSNVNLQEKAYRDGLVEQLYDLTLEYLHSQAHCIGFPELVLPVVLQLKSFLRECKVANYCRQVQQLLGKVQENSAYICSRRQRVSFGVSEQQAVEAWEKLTREEGTPLTLYYSHWRKLRDREIQLEISGKERLEDLNFPEIKRRKMADRKDEDRKQFKDLFDLNSSEEDDTEGFSERGILRPLSTRHGVEDDEEDEEEGEEDSSNSEDGDPDAEAGLAPGELQQLAQGPEDELEDLQLSEDD

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Rabbit
100
Dog
91
Xenopus
73
Zebrafish
73
Chicken
73
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9Y3T9 As Substrate

Site PTM Type Enzyme
T15 Phosphorylation
S22 Phosphorylation
S26 Phosphorylation
S28 Phosphorylation
S30 Phosphorylation
S32 Phosphorylation
S35 Phosphorylation
T40 Phosphorylation
S49 Phosphorylation
S56 Phosphorylation
S58 Phosphorylation
S60 Phosphorylation
S67 Phosphorylation
K70 Ubiquitination
K77 Ubiquitination
S93 Phosphorylation
S98 Phosphorylation
S100 Phosphorylation
S102 Phosphorylation
S103 Phosphorylation
S112 Phosphorylation
S121 Phosphorylation
K144 Ubiquitination
S146 Phosphorylation
T150 Phosphorylation
K168 Ubiquitination
K226 Ubiquitination
S238 Phosphorylation
S240 Phosphorylation
K245 Ubiquitination
T393 Phosphorylation
K498 Ubiquitination
K516 Ubiquitination
Y533 Phosphorylation
K562 Ubiquitination
K576 Ubiquitination
K606 Ubiquitination
T613 Phosphorylation
T616 Phosphorylation
Y618 Phosphorylation
S620 Phosphorylation
S635 Phosphorylation
K637 Ubiquitination
K649 Ubiquitination
K665 Ubiquitination
S672 Phosphorylation
S673 Phosphorylation
T678 Phosphorylation
S682 Phosphorylation
S691 Phosphorylation
S710 Phosphorylation
S711 Phosphorylation
S713 Phosphorylation
S746 Phosphorylation

Research Backgrounds

Function:

Acts as an inhibitor of histone acetyltransferase activity; prevents acetylation of all core histones by the EP300/p300 histone acetyltransferase at p53/TP53-regulated target promoters in a histone deacetylases (HDAC)-independent manner. Acts as a transcription corepressor of p53/TP53- and TP63-mediated transactivation of the p21/CDKN1A promoter. Involved in the regulation of p53/TP53-dependent apoptosis. Associates together with TP63 isoform TA*-gamma to the p21/CDKN1A promoter.

Subcellular Location:

Nucleus>Nucleoplasm. Nucleus>Nucleolus.
Note: Translocates from the nucleoli to the nucleoplasm in presence of several stressors like ultraviolet irradiation and actinomycin-D. Predominantly detected in the nucleoli in non-mitotic cells. Predominantly detected in nucleoplasma in cells undergoing mitosis.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Interacts with p53/TP53. Interacts (via the N- and C-terminus domains) with AURKB (via the middle kinase domain). Interacts with TP63 isoform TA*-gamma (via activation domain). Interacts with histone H3 (via N-terminus and non-acetylated form preferentially). Associates with core histones and nucleosomes.

Family&Domains:

Belongs to the NOC2 family.

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