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$280 100ul
$350 200ul

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  • Product Name
    TNNI3K Antibody
  • Catalog No.
    DF4477
  • RRID
    AB_2836828
  • Source
    Rabbit
  • Application
    WB,IHC,ELISA
  • Reactivity
    Human, Mouse, Rat
  • Prediction
    Zebrafish(100%), Bovine(100%), Horse(100%), Rabbit(100%), Dog(100%), Chicken(100%)
  • UniProt
  • Mol.Wt
    (Observed)0kD; (Calculated)93kDa
  • Concentration
    1mg/ml
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Product Information

Alternative Names:Expand▼

Cardiac ankyrin repeat kinase; cardiac muscle; Cardiac troponin I-interacting kinase; CARK; CMD1FF; CMD2A; CMH7; cTnl; MGC142099; MGC33828; RCM1; Serine/threonine protein kinase TNN13K; Serine/threonine-protein kinase TNNI3K; TNI3K_HUMAN; TNNC1; TNNI3 interacting kinase; TNNI3-interacting kinase; Tnni3k; Troponin 1;

Applications:

IHC: 1:50-1:200, WB 1:500-1:2000, ELISA(peptide) 1:20000-1:40000

Reactivity:

Human, Mouse, Rat

Predicted Reactivity:

Zebrafish(100%), Bovine(100%), Horse(100%), Rabbit(100%), Dog(100%), Chicken(100%)

Source:

Rabbit

Clonality:

Polyclonal

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Specificity:

TNNI3K Antibody detects endogenous levels of total TNNI3K.

RRID:

AB_2836828
Please cite this product as: Affinity Biosciences Cat# DF4477, RRID:AB_2836828.

Format:

Liquid

Concentration:

1mg/ml

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

Immunogen Information

Immunogen:

A synthesized peptide derived from human TNNI3K, corresponding to a region within the internal amino acids.

Uniprot:



>>Visit The Human Protein Atlas

Gene ID:

Gene Name:

TNNI3K

Molecular Weight:

Observed Mol.Wt.: (Observed)0kD.
Predicted Mol.Wt.: (Calculated)93kDa.

Subcellular Location:

Nucleus. Cytoplasm. Expressed at lower levels in the cytoplasm.

Tissue Specificity:

Q59H18 TNI3K_HUMAN:
Highly expressed in both adult and fetal heart.

Sequence:
MGNYKSRPTQTCTDEWKKKVSESYVITIERLEDDLQIKEKELTELRNIFGSDEAFSKVNLNYRTENGLSLLHLCCICGGKKSHIRTLMLKGLRPSRLTRNGFTALHLAVYKDNAELITSLLHSGADIQQVGYGGLTALHIATIAGHLEAADVLLQHGANVNIQDAVFFTPLHIAAYYGHEQVTRLLLKFGADVNVSGEVGDRPLHLASAKGFLNIAKLLMEEGSKADVNAQDNEDHVPLHFCSRFGHHDIVKYLLQSDLEVQPHVVNIYGDTPLHLACYNGKFEVAKEIIQISGTESLTKENIFSETAFHSACTYGKSIDLVKFLLDQNVININHQGRDGHTGLHSACYHGHIRLVQFLLDNGADMNLVACDPSRSSGEKDEQTCLMWAYEKGHDAIVTLLKHYKRPQDELPCNEYSQPGGDGSYVSVPSPLGKIKSMTKEKADILLLRAGLPSHFHLQLSEIEFHEIIGSGSFGKVYKGRCRNKIVAIKRYRANTYCSKSDVDMFCREVSILCQLNHPCVIQFVGACLNDPSQFAIVTQYISGGSLFSLLHEQKRILDLQSKLIIAVDVAKGMEYLHNLTQPIIHRDLNSHNILLYEDGHAVVADFGESRFLQSLDEDNMTKQPGNLRWMAPEVFTQCTRYTIKADVFSYALCLWEILTGEIPFAHLKPAAAAADMAYHHIRPPIGYSIPKPISSLLIRGWNACPEGRPEFSEVVMKLEECLCNIELMSPASSNSSGSLSPSSSSDCLVNRGGPGRSHVAALRSRFELEYALNARSYAALSQSAGQYSSQGLSLEEMKRSLQYTPIDKYGYVSDPMSSMHFHSCRNSSSFEDSS

Research Background

Function:

May play a role in cardiac physiology.

Post-translational Modifications:

Autophosphorylated.

Subcellular Location:

Nucleus. Cytoplasm.
Note: Expressed at lower levels in the cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte

Tissue Specificity:

Highly expressed in both adult and fetal heart.

Subunit Structure:

Interacts with TNNI3, ACTC, ACTA1, MYBPC3, AIP, BABP3 and HADHB.

Similarity:

Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.

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Catalog Number :

DF4477-BP
(Blocking peptide available as DF4477-BP)

Price/Size :

$350/1mg.
Tips: For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Function :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

Format and storage :

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.

Precautions :

This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Chicken
100%
Rabbit
100%
Zebrafish
100%
Dog
100%
Horse
100%
Bovine
100%
Sheep
0%
Xenopus
0%
Pig
0%
High similarity Medium similarity Low similarity No similarity
Q59H18 as Substrate
Site PTM Type Enzyme
K40 Ubiquitination
Y62 Phosphorylation
Y416 Phosphorylation
S424 Phosphorylation
Y425 Phosphorylation
S427 Phosphorylation
T581 Phosphorylation
Y771 Phosphorylation
Y788 Phosphorylation
Y804 Phosphorylation
T805 Phosphorylation
Y810 Phosphorylation
Y812 Phosphorylation
S814 Phosphorylation
IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.

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