TSSK4 Antibody - #DF4563
| Product: | TSSK4 Antibody |
| Catalog: | DF4563 |
| Description: | Rabbit polyclonal antibody to TSSK4 |
| Application: | WB IHC IF/ICC |
| Reactivity: | Human, Mouse |
| Prediction: | Pig, Horse, Sheep, Rabbit, Dog |
| Mol.Wt.: | 40 KD; 37kD(Calculated). |
| Uniprot: | Q6SA08 |
| RRID: | AB_2836914 |
Related Downloads
Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF4563, RRID:AB_2836914.
Fold/Unfold
C14orf20; Serine/threonine-protein kinase 22E; STK22E; Testis-specific kinase 4; Testis-specific serine/threonine-protein kinase 4; TSK-4; TSSK-4; Tssk4; TSSK4_HUMAN; TSSK5;
Immunogens
- Q6SA08 TSSK4_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MGKGDVLEAAPTTTAYHSLMDEYGYEVGKAIGHGSYGSVYEAFYTKQKVMVAVKIISKKKASDDYLNKFLPREIQVMKVLRHKYLINFYRAIESTSRVYIILELAQGGDVLEWIQRYGACSEPLAGKWFSQLTLGIAYLHSKSIVHRDLKLENLLLDKWENVKISDFGFAKMVPSNQPVGCSPSYRQVNCFSHLSQTYCGSFAYACPEILRGLPYNPFLSDTWSMGVILYTLVVAHLPFDDTNLKKLLRETQKEVTFPANHTISQECKNLILQMLRQATKRATILDIIKDSWVLKFQPEQPTHEIRLLEAMCQLHNTTKQHQSLQITT
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q6SA08 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S165 | Phosphorylation | Uniprot | |
| T197 | Phosphorylation | Uniprot | |
| T251 | Phosphorylation | Uniprot | |
| T256 | Phosphorylation | Uniprot | |
| S264 | Phosphorylation | Uniprot | |
| T283 | Phosphorylation | Uniprot |
Research Backgrounds
Serine/threonine kinase which is involved in male germ cell development and in mature sperm function (By similarity). May be involved in the Cre/Creb signaling pathway (By similarity). Phosphorylates CREB1 on 'Ser-133' in vitro and can stimulate Cre/Creb pathway in cells. Phosphorylates CREM on 'Ser-116' in vitro (By similarity). Phosphorylates ODF2 on 'Ser-95' (By similarity).
Activated by autophosphorylation on Thr-197. ODF2 potentiates the autophosphorylation activity of TSSK4 at Thr-197.
Ubiquitinated; HSP90 activity negatively regulates ubiquitination and degradation.
Cytoplasmic vesicle>Secretory vesicle>Acrosome. Cell projection>Cilium>Flagellum.
Note: In spermatozoa, present in the sperm head and in the flagellum.
Expressed only in the testis.
Homodimer (By similarity). Interacts with HSP90; this interaction stabilizes and activates TSSK4 (By similarity). Interacts with ODF2 (via C-terminus); this interaction promotes ODF2 phosphorylation on 'Ser-95' (By similarity). May interact with CREM (By similarity). Interacts with CREB1; this interaction facilitates phosphorylation on 'Ser-133'. Interacts with QRICH2.
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.
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