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$280 100ul
$350 200ul

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  • Product Name
    VTI1A Antibody
  • Catalog No.
    DF4609
  • RRID
    AB_2836973
  • Source
    Rabbit
  • Application
    WB,IF/ICC,ELISA(peptide)
  • Reactivity
    Human, Mouse, Rat
  • Prediction
    Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
  • UniProt
  • Mol.Wt
    23 kD;
    25kDa(Calculated).
  • Concentration
    1mg/ml
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Product Information

Alternative Names:Expand▼

MMDS3; MVti1; SNARE Vti1a beta protein; Vesicle transport through interaction with t-SNAREs homolog 1A; Vesicle transport through interaction with tSNAREs homolog 1A (yeast); Vesicle transport v-SNARE protein Vti1-like 2; Vti1 rp2; Vti1-rp2; Vti1a; VTI1A vesicle transport through interaction with t-SNAREs 1A; VTI1A_HUMAN; VTI1RP2;

Applications:

WB 1:500-1:1000, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000
*The optimal dilutions should be determined by the end user.

Reactivity:

Human, Mouse, Rat

Predicted Reactivity:

Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus

Source:

Rabbit

Clonality:

Polyclonal

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Specificity:

VTI1A Antibody detects endogenous levels of total VTI1A.

RRID:

AB_2836973
Please cite this product as: Affinity Biosciences Cat# DF4609, RRID:AB_2836973.

Format:

Liquid

Concentration:

1mg/ml

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

Immunogen Information in 3D

Immunogen:

A synthesized peptide derived from human VTI1A, corresponding to a region within the internal amino acids.

Uniprot:



>>Visit The Human Protein Atlas

Gene ID:

Gene Name:

VTI1A

Molecular Weight:

Observed Mol.Wt.: 23 kD.
Predicted Mol.Wt.: 25kDa(Calculated)..

Subcellular Location:

Cytoplasmic vesicle. Golgi apparatus membrane.

Sequence:
MSSDFEGYEQDFAVLTAEITSKIARVPRLPPDEKKQMVANVEKQLEEAKELLEQMDLEVREIPPQSRGMYSNRMRSYKQEMGKLETDFKRSRIAYSDEVRNELLGDDGNSSENQRAHLLDNTERLERSSRRLEAGYQIAVETEQIGQEMLENLSHDREKIQRARERLRETDANLGKSSRILTGMLRRIIQNRILLVILGIIVVITILMAITFSVRRH

Research Background

Function:

V-SNARE that mediates vesicle transport pathways through interactions with t-SNAREs on the target membrane. These interactions are proposed to mediate aspects of the specificity of vesicle trafficking and to promote fusion of the lipid bilayers. Involved in vesicular transport from the late endosomes to the trans-Golgi network. Along with VAMP7, involved in an non-conventional RAB1-dependent traffic route to the cell surface used by KCNIP1 and KCND2. May be involved in increased cytokine secretion associated with cellular senescence.

Subcellular Location:

Cytoplasmic vesicle. Golgi apparatus membrane>Single-pass type IV membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte

Subunit Structure:

Interacts with distinct SNARE complexes that contain either STX5 or STX6. Interacts with NAPA and, to a lesser extent, with NAPG. Identified in a complex containing STX6, STX12, VAMP4 and VTI1A.

Similarity:

Belongs to the VTI1 family.

Research Fields

Research Fields:

· Genetic Information Processing > Folding, sorting and degradation > SNARE interactions in vesicular transport.

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Catalog Number :

DF4609-BP
(Blocking peptide available as DF4609-BP)

Price/Size :

$350/1mg.
Tips: For modified antibodies, we provide modified peptides(0.5mg) and non-modified peptides(0.5mg).

Function :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

Format and storage :

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.

Precautions :

This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Pig
100%
Horse
100%
Bovine
100%
Sheep
100%
Dog
100%
Rabbit
100%
Chicken
93%
Xenopus
86%
Zebrafish
86%
High similarity Medium similarity Low similarity No similarity
Q96AJ9 as Substrate
Site PTM Type Enzyme
S3 Phosphorylation
K35 Ubiquitination
K43 Ubiquitination
S66 Phosphorylation
S76 Phosphorylation
K89 Ubiquitination
S91 Phosphorylation
Y95 Phosphorylation
S110 Phosphorylation
T122 Phosphorylation
Y136 Phosphorylation
T170 Phosphorylation
K176 Ubiquitination
S178 Phosphorylation
S213 Phosphorylation
IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.

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