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$280 100ul
$350 200ul

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  • Product Name
    VTI1B Antibody
  • Catalog No.
    DF4610
  • RRID
    AB_2836974
  • Source
    Rabbit
  • Application
    WB,IF/ICC,ELISA(peptide)
  • Reactivity
    Human, Mouse, Rat
  • Prediction
    Pig, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
  • UniProt
  • Mol.Wt
    27 kD;
    27kDa(Calculated).
  • Concentration
    1mg/ml
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Product Information

Alternative Names:Expand▼

HGNC:17793; V SNARE; v-SNARE; Vesicle associated soluble NSF attachment protein receptor; Vesicle transport through interaction with t SNAREs 1B; Vesicle transport through interaction with t SNAREs homolog 1B (yeast); Vesicle transport through interaction with t-SNAREs homolog 1B; Vesicle transport v-SNARE protein Vti1-like 1; VTI1; VTI1 like; Vti1 rp1; VTI1-LIKE; Vti1-rp1; VTI1B; VTI1B_HUMAN; VTI1L; VTI1L1; VTI2;

Applications:

WB 1:500-1:1000, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000
*The optimal dilutions should be determined by the end user.

Reactivity:

Human, Mouse, Rat

Predicted Reactivity:

Pig, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus

Source:

Rabbit

Clonality:

Polyclonal

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Specificity:

VTI1B Antibody detects endogenous levels of total VTI1B.

RRID:

AB_2836974
Please cite this product as: Affinity Biosciences Cat# DF4610, RRID:AB_2836974.

Format:

Liquid

Concentration:

1mg/ml

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

Immunogen Information in 3D

Immunogen:

A synthesized peptide derived from human VTI1B, corresponding to a region within the internal amino acids.

Uniprot:



>>Visit The Human Protein Atlas

Gene ID:

Gene Name:

VTI1B

Molecular Weight:

Observed Mol.Wt.: 27 kD.
Predicted Mol.Wt.: 27kDa(Calculated)..

Subcellular Location:

Endomembrane system. Cytoplasmic granule.

Tissue Specificity:

Q9UEU0 VTI1B_HUMAN:
Expressed in all tissues examined.

Sequence:
MASSAASSEHFEKLHEIFRGLHEDLQGVPERLLGTAGTEEKKKLIRDFDEKQQEANETLAEMEEELRYAPLSFRNPMMSKLRNYRKDLAKLHREVRSTPLTATPGGRGDMKYGIYAVENEHMNRLQSQRAMLLQGTESLNRATQSIERSHRIATETDQIGSEIIEELGEQRDQLERTKSRLVNTSENLSKSRKILRSMSRKVTTNKLLLSIIILLELAILGGLVYYKFFRSH

Research Background

Function:

V-SNARE that mediates vesicle transport pathways through interactions with t-SNAREs on the target membrane. These interactions are proposed to mediate aspects of the specificity of vesicle trafficking and to promote fusion of the lipid bilayers. May be concerned with increased secretion of cytokines associated with cellular senescence.

Subcellular Location:

Early endosome membrane>Single-pass type IV membrane protein. Late endosome membrane>Single-pass type IV membrane protein. Lysosome membrane. Cytoplasmic granule. Recycling endosome membrane>Single-pass type IV membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte

Tissue Specificity:

Expressed in all tissues examined.

Subunit Structure:

Forms a SNARE complex with STX7, STX8 and VAMP8 which functions in the homotypic fusion of late endosomes. Component of the SNARE complex composed of STX7, STX8, VAMP7 and VIT1B that is required for heterotypic fusion of late endosomes with lysosomes (By similarity). May interact with STX17. Interacts with CLINT1.

Similarity:

Belongs to the VTI1 family.

Research Fields

Research Fields:

· Genetic Information Processing > Folding, sorting and degradation > SNARE interactions in vesicular transport.

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Catalog Number :

DF4610-BP
(Blocking peptide available as DF4610-BP)

Price/Size :

$350/1mg.
Tips: For modified antibodies, we provide modified peptides(0.5mg) and non-modified peptides(0.5mg).

Function :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

Format and storage :

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.

Precautions :

This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Pig
100%
Horse
100%
Bovine
100%
Sheep
100%
Rabbit
100%
Dog
91%
Xenopus
82%
Zebrafish
73%
Chicken
0%
High similarity Medium similarity Low similarity No similarity
Q9UEU0 as Substrate
Site PTM Type Enzyme
A2 Acetylation
K13 Ubiquitination
K51 Ubiquitination
S72 Phosphorylation
K90 Ubiquitination
T103 Phosphorylation
R107 Methylation
Y112 Phosphorylation
Y115 Phosphorylation
S127 Phosphorylation
S138 Phosphorylation
T154 Phosphorylation
T156 Phosphorylation
S161 Phosphorylation
S179 Phosphorylation
S185 Phosphorylation
S189 Phosphorylation
K190 Ubiquitination
T203 Phosphorylation
S210 Phosphorylation
Y225 Phosphorylation
IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.

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