IKZF4 Antibody - #DF4672
| Product: | IKZF4 Antibody |
| Catalog: | DF4672 |
| Description: | Rabbit polyclonal antibody to IKZF4 |
| Application: | WB IF/ICC |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken |
| Mol.Wt.: | 64 KD; 64kD(Calculated). |
| Uniprot: | Q9H2S9 |
| RRID: | AB_2837023 |
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Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF4672, RRID:AB_2837023.
Fold/Unfold
EOS; Eos protein; IKAROS family zinc finger 4 (Eos); IKAROS family zinc finger 4; Ikaros family zinc finger protein 4; IKZF4; IKZF4_HUMAN; KIAA1782; Zinc finger protein Eos (IKAROS family zinc finger protein 4); Zinc finger protein Eos; Zinc finger protein, subfamily 1A, 4 (Eos); Zinc finger protein, subfamily 1A, member 4; Zinc finger transcription factor Eos; ZNFN1A4;
Immunogens
Highly expressed in skeletal muscle, low levels of expression in heart, thymus, kidney, liver, and spleen. Expressed in the hematopoietic cell lines MOLT-4, NALM-6 and K-562. Highly expressed in THP-1 and M-07e cell lines, which have characteristics of myeloid and early megakaryocytic cells respectively.
- Q9H2S9 IKZF4_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MHTPPALPRRFQGGGRVRTPGSHRQGKDNLERDPSGGCVPDFLPQAQDSNHFIMESLFCESSGDSSLEKEFLGAPVGPSVSTPNSQHSSPSRSLSANSIKVEMYSDEESSRLLGPDERLLEKDDSVIVEDSLSEPLGYCDGSGPEPHSPGGIRLPNGKLKCDVCGMVCIGPNVLMVHKRSHTGERPFHCNQCGASFTQKGNLLRHIKLHSGEKPFKCPFCNYACRRRDALTGHLRTHSVSSPTVGKPYKCNYCGRSYKQQSTLEEHKERCHNYLQSLSTEAQALAGQPGDEIRDLEMVPDSMLHSSSERPTFIDRLANSLTKRKRSTPQKFVGEKQMRFSLSDLPYDVNSGGYEKDVELVAHHSLEPGFGSSLAFVGAEHLRPLRLPPTNCISELTPVISSVYTQMQPLPGRLELPGSREAGEGPEDLADGGPLLYRPRGPLTDPGASPSNGCQDSTDTESNHEDRVAGVVSLPQGPPPQPPPTIVVGRHSPAYAKEDPKPQEGLLRGTPGPSKEVLRVVGESGEPVKAFKCEHCRILFLDHVMFTIHMGCHGFRDPFECNICGYHSQDRYEFSSHIVRGEHKVG
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9H2S9 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T3 | Phosphorylation | Uniprot | |
| S22 | Phosphorylation | Uniprot | |
| Y104 | Phosphorylation | Uniprot | |
| S105 | Phosphorylation | Uniprot | |
| S109 | Phosphorylation | Uniprot | |
| S110 | Phosphorylation | Uniprot | |
| S125 | Phosphorylation | Uniprot | |
| S148 | Phosphorylation | Uniprot | |
| K158 | Acetylation | Uniprot | |
| T182 | Phosphorylation | Uniprot | |
| K207 | Ubiquitination | Uniprot | |
| S210 | Phosphorylation | Uniprot | |
| K213 | Acetylation | Uniprot | |
| K213 | Sumoylation | Uniprot | |
| K213 | Ubiquitination | Uniprot | |
| S240 | Phosphorylation | Uniprot | |
| S241 | Phosphorylation | Uniprot | |
| T243 | Phosphorylation | Uniprot | |
| Y252 | Phosphorylation | Uniprot | |
| T484 | Phosphorylation | Uniprot | |
| S491 | Phosphorylation | Uniprot | |
| K500 | Sumoylation | Uniprot | |
| T509 | Phosphorylation | Uniprot |
Research Backgrounds
DNA-binding protein that binds to the 5'GGGAATRCC-3' Ikaros-binding sequence. Transcriptional repressor. Interacts with SPI1 and MITF to repress transcription of the CTSK and ACP5 promoters via recruitment of corepressors SIN3A and CTBP2. May be involved in the development of central and peripheral nervous systems. Essential for the inhibitory function of regulatory T-cells (Treg). Mediates FOXP3-mediated gene silencing in regulatory T-cells (Treg) via recruitment of corepressor CTBP1 (By similarity).
Nucleus.
Highly expressed in skeletal muscle, low levels of expression in heart, thymus, kidney, liver, and spleen. Expressed in the hematopoietic cell lines MOLT-4, NALM-6 and K-562. Highly expressed in THP-1 and M-07e cell lines, which have characteristics of myeloid and early megakaryocytic cells respectively.
Self-associates. Interacts with other family members; IKZF1, IKZF2, IKZF3 and IKZF5. Interacts with CTBP2. Interacts with SPI1, MITF, FOXP3 and CTBP1 (By similarity).
The N-terminal zinc fingers are involved in sequence-specific DNA binding and heterotypic associations with other family members.
C-terminal zinc fingers mediate homodimerization.
Belongs to the Ikaros C2H2-type zinc-finger protein family.
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