PKN1 Antibody - #DF4790
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Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF4790, RRID:AB_2837144.
Fold/Unfold
DBK; PAK 1; PAK-1; PAK1; PKC1; PKN ALPHA; PKN; Pkn1; PKN1_HUMAN; PRK1; PRKCL1; Protease activated kinase 1; Protease-activated kinase 1; Protein kinase C like 1; Protein kinase C like PKN; Protein kinase C related kinase 1; Protein kinase C-like 1; Protein kinase C-like PKN; Protein kinase N1; Protein kinase PKN alpha; Protein kinase PKN-alpha; Protein-kinase C-related kinase 1; Serine threonine kinase N; Serine threonine protein kinase N; Serine-threonine protein kinase N; Serine/threonine protein kinase N1; Serine/threonine-protein kinase N1;
Immunogens
Found ubiquitously. Expressed in heart, brain, placenta, lung, skeletal muscle, kidney and pancreas. Expressed in numerous tumor cell lines, especially in breast tumor cells.
- Q16512 PKN1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MASDAVQSEPRSWSLLEQLGLAGADLAAPGVQQQLELERERLRREIRKELKLKEGAENLRRATTDLGRSLGPVELLLRGSSRRLDLLHQQLQELHAHVVLPDPAATHDGPQSPGAGGPTCSATNLSRVAGLEKQLAIELKVKQGAENMIQTYSNGSTKDRKLLLTAQQMLQDSKTKIDIIRMQLRRALQAGQLENQAAPDDTQGSPDLGAVELRIEELRHHFRVEHAVAEGAKNVLRLLSAAKAPDRKAVSEAQEKLTESNQKLGLLREALERRLGELPADHPKGRLLREELAAASSAAFSTRLAGPFPATHYSTLCKPAPLTGTLEVRVVGCRDLPETIPWNPTPSMGGPGTPDSRPPFLSRPARGLYSRSGSLSGRSSLKAEAENTSEVSTVLKLDNTVVGQTSWKPCGPNAWDQSFTLELERARELELAVFWRDQRGLCALKFLKLEDFLDNERHEVQLDMEPQGCLVAEVTFRNPVIERIPRLRRQKKIFSKQQGKAFQRARQMNIDVATWVRLLRRLIPNATGTGTFSPGASPGSEARTTGDISVEKLNLGTDSDSSPQKSSRDPPSSPSSLSSPIQESTAPELPSETQETPGPALCSPLRKSPLTLEDFKFLAVLGRGHFGKVLLSEFRPSGELFAIKALKKGDIVARDEVESLMCEKRILAAVTSAGHPFLVNLFGCFQTPEHVCFVMEYSAGGDLMLHIHSDVFSEPRAIFYSACVVLGLQFLHEHKIVYRDLKLDNLLLDTEGYVKIADFGLCKEGMGYGDRTSTFCGTPEFLAPEVLTDTSYTRAVDWWGLGVLLYEMLVGESPFPGDDEEEVFDSIVNDEVRYPRFLSAEAIGIMRRLLRRNPERRLGSSERDAEDVKKQPFFRTLGWEALLARRLPPPFVPTLSGRTDVSNFDEEFTGEAPTLSPPRDARPLTAAEQAAFLDFDFVAGGC
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q16512 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| A2 | Acetylation | Uniprot | |
| S3 | Phosphorylation | Uniprot | |
| S8 | Phosphorylation | Uniprot | |
| K53 | Ubiquitination | Uniprot | |
| T63 | Phosphorylation | Uniprot | |
| T64 | Phosphorylation | Q16512 (PKN1) | Uniprot |
| S69 | Phosphorylation | Uniprot | |
| S80 | Phosphorylation | Uniprot | |
| S112 | Phosphorylation | Uniprot | |
| S205 | Phosphorylation | Uniprot | |
| S240 | Phosphorylation | Uniprot | |
| K243 | Ubiquitination | Uniprot | |
| K256 | Ubiquitination | Uniprot | |
| K263 | Ubiquitination | Uniprot | |
| S301 | Phosphorylation | Uniprot | |
| T311 | Phosphorylation | Uniprot | |
| T315 | Phosphorylation | Uniprot | |
| T353 | Phosphorylation | Uniprot | |
| R357 | Methylation | Uniprot | |
| R366 | Methylation | Uniprot | |
| S372 | Phosphorylation | Uniprot | |
| S374 | Phosphorylation | Uniprot | |
| S376 | Phosphorylation | Q16512 (PKN1) | Uniprot |
| S379 | Phosphorylation | Uniprot | |
| S380 | Phosphorylation | Uniprot | |
| K445 | Ubiquitination | Uniprot | |
| K448 | Acetylation | Uniprot | |
| S495 | Phosphorylation | Uniprot | |
| K496 | Ubiquitination | Uniprot | |
| T527 | Phosphorylation | Uniprot | |
| T529 | Phosphorylation | Uniprot | |
| T531 | Phosphorylation | Uniprot | |
| S533 | Phosphorylation | Uniprot | |
| S537 | Phosphorylation | Uniprot | |
| S540 | Phosphorylation | Uniprot | |
| S549 | Phosphorylation | Uniprot | |
| T557 | Phosphorylation | Uniprot | |
| S559 | Phosphorylation | Uniprot | |
| S561 | Phosphorylation | Uniprot | |
| S562 | Phosphorylation | Uniprot | |
| K565 | Ubiquitination | Uniprot | |
| S573 | Phosphorylation | Uniprot | |
| S575 | Phosphorylation | Uniprot | |
| S576 | Phosphorylation | Uniprot | |
| K607 | Ubiquitination | Uniprot | |
| S608 | Phosphorylation | Uniprot | |
| T611 | Phosphorylation | Uniprot | |
| K644 | Ubiquitination | Uniprot | |
| K664 | Ubiquitination | Uniprot | |
| K742 | Ubiquitination | Uniprot | |
| K763 | Ubiquitination | Uniprot | |
| Y768 | Phosphorylation | Uniprot | |
| T772 | Phosphorylation | Uniprot | |
| S773 | Phosphorylation | O15530 (PDPK1) | Uniprot |
| T774 | Phosphorylation | Q16512 (PKN1) , O15530 (PDPK1) | Uniprot |
| T778 | Phosphorylation | Uniprot | |
| R847 | Methylation | Uniprot | |
| S902 | Phosphorylation | Uniprot | |
| T914 | Phosphorylation | Uniprot | |
| S916 | Phosphorylation | Uniprot | |
| T925 | Phosphorylation | Uniprot |
PTMs - Q16512 As Enzyme
| Substrate | Site | Source |
|---|---|---|
| O95863 (SNAI1) | S246 | Uniprot |
| P00533 (EGFR) | T678 | Uniprot |
| P04049 (RAF1) | S338 | Uniprot |
| P04049 (RAF1) | S339 | Uniprot |
| P10636-6 (MAPT) | T147 | Uniprot |
| P10636-2 (MAPT) | S156 | Uniprot |
| P10636-2 (MAPT) | S200 | Uniprot |
| P10636-8 (MAPT) | S214 | Uniprot |
| P10636-2 (MAPT) | S231 | Uniprot |
| P10636-8 (MAPT) | S258 | Uniprot |
| P10636-6 (MAPT) | S262 | Uniprot |
| P10636-2 (MAPT) | S263 | Uniprot |
| P10636-6 (MAPT) | S294 | Uniprot |
| P10636-8 (MAPT) | S320 | Uniprot |
| P10636-8 (MAPT) | S352 | Uniprot |
| P10636 (MAPT) | S637 | Uniprot |
| P18669 (PGAM1) | S23 | Uniprot |
| P18669 (PGAM1) | S118 | Uniprot |
| P29966 (MARCKS) | S159 | Uniprot |
| P29966 (MARCKS) | S163 | Uniprot |
| P29966 (MARCKS) | S170 | Uniprot |
| P30307 (CDC25C) | S216 | Uniprot |
| P30622 (CLIP1) | S312 | Uniprot |
| P36871 (PGM1) | T467 | Uniprot |
| Q12778 (FOXO1) | S256 | Uniprot |
| Q13077 (TRAF1) | S146 | Uniprot |
| Q16512-1 (PKN1) | T64 | Uniprot |
| Q16512-2 (PKN1) | T70 | Uniprot |
| Q16512-1 (PKN1) | S372 | Uniprot |
| Q16512-1 (PKN1) | S374 | Uniprot |
| Q16512 (PKN1) | S376 | Uniprot |
| Q16512-2 (PKN1) | S378 | Uniprot |
| Q16512-1 (PKN1) | S380 | Uniprot |
| Q16512-1 (PKN1) | T531 | Uniprot |
| Q16512-2 (PKN1) | T537 | Uniprot |
| Q16512-1 (PKN1) | S576 | Uniprot |
| Q16512-2 (PKN1) | S582 | Uniprot |
| Q16512-1 (PKN1) | S608 | Uniprot |
| Q16512-2 (PKN1) | S614 | Uniprot |
| Q16512 (PKN1) | T774 | Uniprot |
| Q92974 (ARHGEF2) | S886 | Uniprot |
| Q9UKV0 (HDAC9) | S253 | Uniprot |
| Q9UQL6 (HDAC5) | T292 | Uniprot |
Research Backgrounds
PKC-related serine/threonine-protein kinase involved in various processes such as regulation of the intermediate filaments of the actin cytoskeleton, cell migration, tumor cell invasion and transcription regulation. Part of a signaling cascade that begins with the activation of the adrenergic receptor ADRA1B and leads to the activation of MAPK14. Regulates the cytoskeletal network by phosphorylating proteins such as VIM and neurofilament proteins NEFH, NEFL and NEFM, leading to inhibit their polymerization. Phosphorylates 'Ser-575', 'Ser-637' and 'Ser-669' of MAPT/Tau, lowering its ability to bind to microtubules, resulting in disruption of tubulin assembly. Acts as a key coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and specifically mediating phosphorylation of 'Thr-11' of histone H3 (H3T11ph), a specific tag for epigenetic transcriptional activation that promotes demethylation of histone H3 'Lys-9' (H3K9me) by KDM4C/JMJD2C. Phosphorylates HDAC5, HDAC7 and HDAC9, leading to impair their import in the nucleus. Phosphorylates 'Thr-38' of PPP1R14A, 'Ser-159', 'Ser-163' and 'Ser-170' of MARCKS, and GFAP. Able to phosphorylate RPS6 in vitro.
Autophosphorylated; preferably on serine. Phosphorylated during mitosis.
Activated by limited proteolysis with trypsin.
(Microbial infection) In case of infection, polyubiquitinated by the bacterial E3 ubiquitin-protein ligase SspH1, leading to its proteasomal degradation.
Cytoplasm. Nucleus. Endosome. Cell membrane>Peripheral membrane protein. Cleavage furrow. Midbody.
Note: Associates with chromatin in a ligand-dependent manner. Localization to endosomes is mediated via its interaction with RHOB. Association to the cell membrane is dependent on Ser-377 phosphorylation. Accumulates during telophase at the cleavage furrow and finally concentrates around the midbody in cytokinesis.
Found ubiquitously. Expressed in heart, brain, placenta, lung, skeletal muscle, kidney and pancreas. Expressed in numerous tumor cell lines, especially in breast tumor cells.
Interacts with ZFAND6 (By similarity). Interacts with ANDR. Interacts with PRKCB. Interacts (via REM 1 and REM 2 repeats) with RAC1. Interacts (via REM 1 repeat) with RHOA. Interacts with RHOB. Interacts (via C-terminus) with PDPK1. Interacts with CCNT2; enhances MYOD1-dependent transcription. Component of a signaling complex containing at least AKAP13, PKN1, MAPK14, ZAK and MAP2K3. Within this complex, AKAP13 interacts directly with PKN1, which in turn recruits MAPK14, MAP2K3 and ZAK.
(Microbial infection) Interacts (via the second REM repeat) with S.typhimurium E3 ubiquitin-protein ligase SspH1 (via the leucine-rich repeat region).
The C1 domain does not bind the diacylglycerol (DAG).
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
Research Fields
· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway. (View pathway)
· Human Diseases > Infectious diseases: Bacterial > Salmonella infection.
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