CLM-1 Antibody | Affinity Biosciences

WB
IF/ICC

1/2

Western blot analysis of extracts from A375 cells, using CLM-1 Antibody.

Product:	CLM-1 Antibody
Catalog:	DF4813
Description:	Rabbit polyclonal antibody to CLM-1
Application:	WB IF/ICC
Reactivity:	Human
Mol.Wt.:	30 KD; 32kD(Calculated).
Uniprot:	Q8TDQ1
RRID:	AB_2837164

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Clonality:

Polyclonal

Specificity:

CLM-1 Antibody detects endogenous levels of total CLM-1.

RRID:

AB_2837164
Cite Format: Affinity Biosciences Cat# DF4813, RRID:AB_2837164.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

CD300 antigen like family member; CD300 antigen-like family member F; CD300 molecule like family member f; CD300f; CD300LF; CLM; CLM-1; CLM1; CLM1_HUMAN; CMRF35-like molecule 1; IGSF; IgSF13; Immune receptor expressed on myeloid cells 1; immune receptor expressed on myeloid cells; immunoglobin superfamily member; Immunoglobulin superfamily member 13; Immunoglobulin superfamily member; Inhibitory receptor IREM1; IREM; IREM-1; IREM1; Nepmucin; NK inhibitory receptor; NKIR; TREM; triggering receptors expressed on myeloid cells; UNQ3105/PRO10111;

Immunogens

Immunogen:

Uniprot:

Q8TDQ1(CLM1_HUMAN) >>Visit HPA database.

Gene(ID):

CD300LF(146722)

Expression:

Q8TDQ1 CLM1_HUMAN:

Highly expressed in spleen, peripheral blood leukocyte and monocyte, and lung. Weakly expressed in thymus, heart, brain, placenta, liver, skeletal muscle, kidney, pancreas, prostate, testis, ovary, small intestine or colon. Expressed selectively in monocytes and monocyte-related cells.

Sequence:

Q8TDQ1 CLM1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MPLLTLYLLLFWLSGYSIVTQITGPTTVNGLERGSLTVQCVYRSGWETYLKWWCRGAIWRDCKILVKTSGSEQEVKRDRVSIKDNQKNRTFTVTMEDLMKTDADTYWCGIEKTGNDLGVTVQVTIDPAPVTQEETSSSPTLTGHHLDNRHKLLKLSVLLPLIFTILLLLLVAASLLAWRMMKYQQKAAGMSPEQVLQPLEGDLCYADLTLQLAGTSPQKATTKLSSAQVDQVEVEYVTMASLPKEDISYASLTLGAEDQEPTYCNMGHLSSHLPGRGPEEPTEYSTISRP

PTMs - Q8TDQ1 As Substrate

Q8TDQ1

Site	PTM Type	Enzyme	Source
K76	Acetylation		Uniprot
Y205	Phosphorylation		Uniprot
Y236	Phosphorylation	P06241 (FYN)	Uniprot
Y249	Phosphorylation		Uniprot
Y263	Phosphorylation	P06241 (FYN)	Uniprot
Y284	Phosphorylation		Uniprot

Research Backgrounds

Q8TDQ1_CLM1_HUMAN

Function:

Acts as an inhibitory receptor for myeloid cells and mast cells. Positively regulates the phagocytosis of apoptotic cells (efferocytosis) via phosphatidylserine (PS) recognition; recognizes and binds PS as a ligand which is expressed on the surface of apoptotic cells. Plays an important role in the maintenance of immune homeostasis, by promoting macrophage-mediated efferocytosis and by inhibiting dendritic cell-mediated efferocytosis (By similarity). Negatively regulates Fc epsilon receptor-dependent mast cell activation and allergic responses via binding to ceramide and sphingomyelin which act as ligands. May act as a coreceptor for interleukin 4 (IL-4). Associates with and regulates IL-4 receptor alpha-mediated responses by augmenting IL-4- and IL-13-induced signaling (By similarity). Negatively regulates the Toll-like receptor (TLR) signaling mediated by MYD88 and TRIF through activation of PTPN6/SHP-1 and PTPN11/SHP-2. Inhibits osteoclast formation. Induces macrophage cell death upon engagement (By similarity).

PTMs:

Phosphorylated on tyrosine.

Subcellular Location:

Cell membrane>Single-pass type I membrane protein.

Tissue Specificity:

Subunit Structure:

Interacts with PTPN6/SHP-1 in a tyrosine phosphorylation dependent manner. Interacts with IL4R (By similarity).

Family&Domains:

Belongs to the CD300 family.

Restrictive clause

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For Research Use Only.
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CLM-1 Antibody - #DF4813