Product: AGTR1 Antibody
Catalog: DF4910
Source: Rabbit
Application: WB, IHC, IF/ICC, ELISA(peptide)
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 41 kD; 41kD(Calculated).
Uniprot: P30556
RRID: AB_2837263

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 100ul $280 In stock
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Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:100-200, ELISA(peptide) 1:20000-1:40000
*The optimal dilutions should be determined by the end user.
Reactivity:
Human,Mouse,Rat
Prediction:
Pig(92%), Bovine(100%), Horse(92%), Sheep(100%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
AGTR1 Antibody detects endogenous levels of total AGTR1.
RRID:
AB_2837263
Cite Format: Affinity Biosciences Cat# DF4910, RRID:AB_2837263.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

AG2S; Agtr 1; Agtr1; AGTR1_HUMAN; Agtr1a; AGTR1B; Ang II; Angiotensin II receptor type 1; Angiotensin II type-1 receptor; Angiotensin receptor 1; Angiotensin receptor 1B; AT 1B; AT 1r; AT1; At1a; AT1AR; AT1B; AT1BR; AT1R; AT2R1; AT2R1A; AT2R1B; HAT1R; Type 1 angiotensin II receptor; Type 1B angiotensin II receptor; Type-1 angiotensin II receptor;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
P30556 AGTR1_HUMAN:

Liver, lung, adrenal and adrenocortical adenomas.

Sequence:
MILNSSTEDGIKRIQDDCPKAGRHNYIFVMIPTLYSIIFVVGIFGNSLVVIVIYFYMKLKTVASVFLLNLALADLCFLLTLPLWAVYTAMEYRWPFGNYLCKIASASVSFNLYASVFLLTCLSIDRYLAIVHPMKSRLRRTMLVAKVTCIIIWLLAGLASLPAIIHRNVFFIENTNITVCAFHYESQNSTLPIGLGLTKNILGFLFPFLIILTSYTLIWKALKKAYEIQKNKPRNDDIFKIIMAIVLFFFFSWIPHQIFTFLDVLIQLGIIRDCRIADIVDTAMPITICIAYFNNCLNPLFYGFLGKKFKRYFLQLLKYIPPKAKSHSNLSTKMSTLSYRPSDNVSSSTKKPAPCFEVE

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Pig
92
Horse
92
Chicken
60
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P30556 As Substrate

Site PTM Type Enzyme
N4 N-Glycosylation
Y26 Phosphorylation
K230 Ubiquitination
C289 S-Nitrosylation
Y302 Phosphorylation
Y319 Phosphorylation P12931 (SRC)
T332 Phosphorylation
S335 Phosphorylation
T336 Phosphorylation
S338 Phosphorylation
S342 Phosphorylation

Research Backgrounds

Function:

Receptor for angiotensin II. Mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system.

PTMs:

C-terminal Ser or Thr residues may be phosphorylated.

Subcellular Location:

Cell membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Liver, lung, adrenal and adrenocortical adenomas.

Subunit Structure:

Interacts with MAS1 (Probable). Interacts with ARRB1 (By similarity). Interacts with FLNA (via filamin repeat 21); increases PKA-mediated phosphorylation of FLNA.

Family&Domains:

Belongs to the G-protein coupled receptor 1 family.

Research Fields

· Environmental Information Processing > Signal transduction > Calcium signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > cGMP-PKG signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Phospholipase D signaling pathway.   (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > Neuroactive ligand-receptor interaction.

· Environmental Information Processing > Signal transduction > Apelin signaling pathway.   (View pathway)

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Organismal Systems > Circulatory system > Adrenergic signaling in cardiomyocytes.   (View pathway)

· Organismal Systems > Circulatory system > Vascular smooth muscle contraction.   (View pathway)

· Organismal Systems > Endocrine system > Renin-angiotensin system.   (View pathway)

· Organismal Systems > Endocrine system > Renin secretion.

· Organismal Systems > Endocrine system > Aldosterone synthesis and secretion.

References

1). Lian FZ et al. Xin-Ji-Er-Kang ameliorates kidney injury following myocardial infarction by inhibiting oxidative stress via Nrf2/HO-1 pathway in rats. Biomed Pharmacother 2019 Jun 19;117:109124 (PubMed: 31228798) [IF=4.545]

Application: WB    Species: rat    Sample: renal cortical

Fig. 10. | Effect of XJEK on AT1R and NOX-4 levels in renal cortical tissues of MI rats. The concentration levels of Ang II type 1 receptor (AT1R) and NADPH Oxidase-4 (NOX-4) proteins in renal cortical tissues by Western blot analysis and commercial ELISA kits. (A, B and C) AT1R content in renal cortical tissues of rats for 2, 4 and 6wk post-MI by western blot, respectively (mean ± SEM, n = 4 in each group)

2). Galan A et al. G3BP1 interacts directly with the FMDV IRES and negatively regulates translation. FEBS J 2017 Oct;284(19):3202-3217 (PubMed: 28755480) [IF=4.392]

3). Shi X et al. MiR-144-5p limits experimental abdominal aortic aneurysm formation by mitigating M1 macrophage-associated inflammation: Suppression of TLR2 and OLR1. J Mol Cell Cardiol 2020 Jun;143:1-14. (PubMed: 32278833) [IF=4.133]

Application: WB    Species: mouse    Sample:

Fig. S3.|AT1R expression was analyzed via Western blot. AT1R expression increased 2.5-fold in response to Ang II infusion, which was slightly decreased by miR144-5p agomirs.

4). Gu L et al. A network-based analysis of key pharmacological pathways of Andrographis paniculata acting on Alzheimer's disease and experimental validation. J Ethnopharmacol 2019 Dec 20;251:112488 (PubMed: 31866509) [IF=3.690]

5). Wang X et al. AT1R regulates macrophage polarization through YAP and regulates aortic dissection incidence. Front Physiol 2021 Jul 9;12:644903. (PubMed: 34305627) [IF=3.367]

Application: WB    Species: Mice    Sample: aortic tissue

FIGURE 8 ELISA and Western Blot results of aortic tissue in each group of mice. (A) ET-1 ELISA results were shown. Both telmisartan and nifedipine treatments can effectively reduce ET-1 serum levels in the AD mice model. Telmisartan is slightly better than nifedipine. (B) IL-6 ELISA results were shown. Both telmisartan and nifedipine treatments can effectively reduce IL-6 serum levels in the AD mice model. Telmisartan is slightly better than nifedipine. (C) MMP 9 ELISA results were shown. Both telmisartan and nifedipine treatments can effectively reduce MMP 9 serum levels in the AD mice model. Telmisartan is slightly better than nifedipine. (D) Western Blot results of YAP and AT1R content in aortic tissue of each group were demonstrated. The contents of AT1R and p-YAP in the aortic tissue of AD group were significantly increased, and the total content of YAP was decreased. Telmisartan treatment can effectively alleviate this phenomenon, and nifedipine cannot affect the expression of AT1R and YAP. (n = 3, mean and S.D., t-test, *P < 0.01 compared with control group; **P < 0.05 compared with the control group; ∧∧P < 0.05 compared with the AD group; ##P < 0.05 compared with the AD + telmisartan group).

6). Wang X et al. Angiotensin type 1 receptor regulates yes-associated protein in vascular endothelial cells. Exp Ther Med 2020 Jan;19 (PubMed: 31885711)

Application: WB    Species: human    Sample: HAECs

Figure 3. |Ang II promotes AT1R expression and YAP phosphorylation (Ser127), and treatment with telmisartan or transfection with AT1R siRNA reverses this effect. Grouping: Control group, HAECs only; group 1, HAECs with Ang II (1 µM) treatment; group 2, HAECs with Ang II (1 µM) and siRNA NC transfection; group 3, HAECs with Ang II (1 µM) and AT1R siRNA transfection; group 4, HAECs with Ang II (1 µM) and 1 µl DMSO; and group 5, HAECs with Ang II (1 µM) and the ARB 1 µl (20 mM) telmisartan treatment. (A) Western blot analysis of sets of six independent lysates from HAECs that were untreated, treated with Ang II, treated with Ang II and siRNA NC, treated with Ang II and AT1R siRNA, treated with Ang II and DMSO, or treated with Ang II and telmisartan. Treatment with Ang II upregulated AT1R and p‑YAP, and this effect was alleviated by transfection with an AT1R siRNA or treatment with telmisartan.

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