Product: Patched/PTCH1 Antibody
Catalog: AF5202
Source: Rabbit
Application: WB, IHC, IF/ICC, IHC-P, ELISA(peptide)
Reactivity: Human, Mouse, Rat
Mol.Wt.: 160kD; 161kD(Calculated).
Uniprot: Q13635
RRID: AB_2837688

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC-p 1:50-1:200, IF/ICC 1:100-1:500, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000
*The optimal dilutions should be determined by the end user.
Reactivity:
Human,Mouse,Rat
Clonality:
Polyclonal
Specificity:
Patched/PTCH1 Antibody detects endogenous levels of total Patched.
RRID:
AB_2837688
Cite Format: Affinity Biosciences Cat# AF5202, RRID:AB_2837688.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

A230106A15Rik; BCNS; FLJ26746; FLJ42602; Holoprosencephaly 7; HPE7; mes; NBCCS; OTTHUMP00000021709; OTTHUMP00000021710; Patched; Patched (Drosophila) homolog; Patched 1; Patched homolog (Drosophila); Patched homolog 1 (Drosophila); Patched homolog 1; Patched protein homolog 1; Protein patched homolog 1; PTC; PTC1; PTC1_HUMAN; PTCH; PTCH protein +12b; PTCH protein +4'; PTCH protein -10; PTCH protein; ptch1; PTCH1 protein; PTCH11;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
Q13635 PTC1_HUMAN:

In the adult, expressed in brain, lung, liver, heart, placenta, skeletal muscle, pancreas and kidney. Expressed in tumor cells but not in normal skin.

Description:
Acts as a receptor for sonic hedgehog (SHH), indian hedgehog (IHH) and desert hedgehog (DHH). Associates with the smoothened protein (SMO) to transduce the hedgehog's proteins signal. Seems to have a tumor suppressor function, as inactivation of this protein is probably a necessary, if not sufficient step for tumorigenesis
Sequence:
MASAGNAAEPQDRGGGGSGCIGAPGRPAGGGRRRRTGGLRRAAAPDRDYLHRPSYCDAAFALEQISKGKATGRKAPLWLRAKFQRLLFKLGCYIQKNCGKFLVVGLLIFGAFAVGLKAANLETNVEELWVEVGGRVSRELNYTRQKIGEEAMFNPQLMIQTPKEEGANVLTTEALLQHLDSALQASRVHVYMYNRQWKLEHLCYKSGELITETGYMDQIIEYLYPCLIITPLDCFWEGAKLQSGTAYLLGKPPLRWTNFDPLEFLEELKKINYQVDSWEEMLNKAEVGHGYMDRPCLNPADPDCPATAPNKNSTKPLDMALVLNGGCHGLSRKYMHWQEELIVGGTVKNSTGKLVSAHALQTMFQLMTPKQMYEHFKGYEYVSHINWNEDKAAAILEAWQRTYVEVVHQSVAQNSTQKVLSFTTTTLDDILKSFSDVSVIRVASGYLLMLAYACLTMLRWDCSKSQGAVGLAGVLLVALSVAAGLGLCSLIGISFNAATTQVLPFLALGVGVDDVFLLAHAFSETGQNKRIPFEDRTGECLKRTGASVALTSISNVTAFFMAALIPIPALRAFSLQAAVVVVFNFAMVLLIFPAILSMDLYRREDRRLDIFCCFTSPCVSRVIQVEPQAYTDTHDNTRYSPPPPYSSHSFAHETQITMQSTVQLRTEYDPHTHVYYTTAEPRSEISVQPVTVTQDTLSCQSPESTSSTRDLLSQFSDSSLHCLEPPCTKWTLSSFAEKHYAPFLLKPKAKVVVIFLFLGLLGVSLYGTTRVRDGLDLTDIVPRETREYDFIAAQFKYFSFYNMYIVTQKADYPNIQHLLYDLHRSFSNVKYVMLEENKQLPKMWLHYFRDWLQGLQDAFDSDWETGKIMPNNYKNGSDDGVLAYKLLVQTGSRDKPIDISQLTKQRLVDADGIINPSAFYIYLTAWVSNDPVAYAASQANIRPHRPEWVHDKADYMPETRLRIPAAEPIEYAQFPFYLNGLRDTSDFVEAIEKVRTICSNYTSLGLSSYPNGYPFLFWEQYIGLRHWLLLFISVVLACTFLVCAVFLLNPWTAGIIVMVLALMTVELFGMMGLIGIKLSAVPVVILIASVGIGVEFTVHVALAFLTAIGDKNRRAVLALEHMFAPVLDGAVSTLLGVLMLAGSEFDFIVRYFFAVLAILTILGVLNGLVLLPVLLSFFGPYPEVSPANGLNRLPTPSPEPPPSVVRFAMPPGHTHSGSDSSDSEYSSQTTVSGLSEELRHYEAQQGAGGPAHQVIVEATENPVFAHSTVVHPESRHHPPSNPRQQPHLDSGSLPPGRQGQQPRRDPPREGLWPPPYRPRRDAFEISTEGHSGPSNRARWGPRGARSHNPRNPASTAMGSSVPGYCQPITTVTASASVTVAVHPPPVPGPGRNPRGGLCPGYPETDHGLFEDPHVPFHVRCERRDSKVEVIELQDVECEERPRGSSSN

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
67
Chicken
63
Pig
0
Horse
0
Bovine
0
Sheep
0
Dog
0
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q13635 As Substrate

Site PTM Type Enzyme
Ubiquitination
K163 Ubiquitination
T368 Phosphorylation
S435 Phosphorylation
S463 Phosphorylation
K993 Ubiquitination
T1195 Phosphorylation
S1197 Phosphorylation
S1331 Phosphorylation
R1394 Methylation
S1425 Phosphorylation

Research Backgrounds

Function:

Acts as a receptor for sonic hedgehog (SHH), indian hedgehog (IHH) and desert hedgehog (DHH). Associates with the smoothened protein (SMO) to transduce the hedgehog's proteins signal. Seems to have a tumor suppressor function, as inactivation of this protein is probably a necessary, if not sufficient step for tumorigenesis.

PTMs:

Glycosylation is necessary for SHH binding.

In the absence of Hh ligands, ubiquitination by ITCH at Lys-1426 promotes endocytosis and both proteasomal and lysosomal degradation.

Subcellular Location:

Cell membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

In the adult, expressed in brain, lung, liver, heart, placenta, skeletal muscle, pancreas and kidney. Expressed in tumor cells but not in normal skin.

Subunit Structure:

Interacts with SNX17. Interacts with IHH. Interacts with G-protein coupled receptor GPR37L1 (By similarity).

Family&Domains:

Belongs to the patched family.

Research Fields

· Environmental Information Processing > Signal transduction > cAMP signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Hedgehog signaling pathway.   (View pathway)

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

· Human Diseases > Cancers: Specific types > Basal cell carcinoma.   (View pathway)

· Organismal Systems > Development > Axon guidance.   (View pathway)

References

1). Li S et al. Silica Perturbs Primary Cilia and Causes Myofibroblast Differentiation during Silicosis by Reduction of the KIF3A-Repressor GLI3 Complex. Theranostics 2020 Jan 1;10(4):1719-1732 (PubMed: 32042332) [IF=8.579]

Application: WB    Species: human    Sample: MRC-5 fibroblasts

Figure 6. Detection of SHH signalling proteins in MRC-5 fibroblasts in response to SiO2 stimulation and KIF3A knockdown. (A, B) Western blot and densitometric analyses of SHH, PTC1, SMO, and GLI1 proteins in MRC-5 fibroblasts treated with SiO2 for 12 h together with KIF3A knockdown until 36 h. The relative density was normalised to α-Tub. Bar graphs are the means±SD of three separate experiments. *P<0.05; **P<0.01. Data are the mean±SD. Statistical analysis was performed using one-way ANOVA and SPSS 20.0. (C) Schematic of the primary cilia influence on SHH signalling. Bidirectional transcriptional factors regulate the transcription of target genes.

Application: WB    Species: Human    Sample: MRC-5 fibroblasts

Figure 6 Detection of SHH signalling proteins in MRC-5 fibroblasts in response to SiO2 stimulation and KIF3A knockdown. (A, B) Western blot and densitometric analyses of SHH, PTC1, SMO, and GLI1 proteins in MRC-5 fibroblasts treated with SiO2 for 12 h together with KIF3A knockdown until 36 h. The relative density was normalised to α-Tub. Bar graphs are the means±SD of three separate experiments. *P<0.05; **P<0.01. Data are the mean±SD. Statistical analysis was performed using one-way ANOVA and SPSS 20.0. (C) Schematic of the primary cilia influence on SHH signalling. Bidirectional transcriptional factors regulate the transcription of target genes.

2). Li S et al. Silica Perturbs Primary Cilia and Causes Myofibroblast Differentiation during Silicosis by Reduction of the KIF3A-Repressor GLI3 Complex. Theranostics 2020 Jan 1;10(4):1719-1732 (PubMed: 32042332) [IF=8.579]

3). Jiang Y et al. Hedgehog pathway inhibition causes primary follicle atresia and decreases female germline stem cell proliferation capacity or stemness. Stem Cell Res Ther 2019 Jul 5;10(1):198 (PubMed: 31277696) [IF=5.116]

Application: WB    Species: mouse    Sample: ovarian

Fig. 1 | Hh signaling pathway activity decreases with ovarian aging. a mRNA expression levels of Gli1, Ptch1, and Cyclin D1 in three mice of reproductive age. b, c Protein expression levels.

Application: IHC    Species: mouse    Sample: ovarian

Fig. 1 | Hh signaling pathway activity decreases with ovarian aging. a mRNA expression levels of Gli1, Ptch1, and Cyclin D1 in three mice of reproductive age. b, c Protein expression levels.d IHC detection of key members of the Hh pathway, Gli1, Ptch1, and Cyclin D1, in the OSE. Bar is 10 μm. *p < 0.05 and **p < 0.01

Application: WB    Species: mouse    Sample: ovaries

Fig. |1 Hh signaling pathway activity decreases with ovarian aging. a mRNA expression levels of Gli1, Ptch1, and Cyclin D1 in three mice of reproductive age. b, c Protein expression levels.

Application: IHC    Species: mouse    Sample: ovaries

Fig. |1 Hh signaling pathway activity decreases with ovarian aging. a mRNA expression levels of Gli1, Ptch1, and Cyclin D1 in three mice of reproductive age. b, c Protein expression levels.d IHC detection of key members of the Hh pathway, Gli1, Ptch1, and Cyclin D1, in the OSE. Bar is 10 μm. *p < 0.05 and **p < 0.01

4). Wang C et al. Gankyrin activates the hedgehog signalling to drive metastasis in osteosarcoma. J Cell Mol Med 2021 Jun 5. (PubMed: 34089292) [IF=4.486]

5). Zhang B et al. Targeting the RAS axis alleviates silicotic fibrosis and Ang II-induced myofibroblast differentiation via inhibition of the hedgehog signaling pathway. Toxicol Lett 2019 Oct 1;313:30-41 (PubMed: 31181250) [IF=3.569]

6). Fan J et al. Curcumin mitigates the epithelial-to-mesenchymal transition in biliary epithelial cells through upregulating CD109 expression. Drug Dev Res 2019 Aug 12 (PubMed: 31403228)

Application: WB    Species: human    Sample: HIBECs

FIGURE 5 |Curcumin suppresses the TGF-β1-activated Hedgehog signaling in HIBECs. (a) Quantitative analysis of Shh, Gli1, Smo, Ptch-1, and Sufu protein expression.

7). Hu A et al. Metformin exerts anti-tumor effects via Sonic hedgehog signaling pathway by targeting AMPK in HepG2 cells. Biochem Cell Biol 2022 Jan 6;1-10. (PubMed: 34990285)

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