Ki67 Antibody | Affinity Biosciences

WB
IHC
Cites

1/3

Western blot analysis of ki67 expression in A431 cells.

AF5208 at 1/100 staining human Lymph node tissue sections by IHC-P.

Figure 2 Representative images of the pathological staining of MAA.

Product:	Ki67 Antibody
Catalog:	AF5208
Description:	Rabbit polyclonal antibody to Ki67
Application:	WB IHC IF/ICC
Reactivity:	Human
Mol.Wt.:	358kDa; 359kD(Calculated).
Uniprot:	P46013
RRID:	AB_2837694

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 500-2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Clonality:

Polyclonal

Specificity:

Ki67 Antibody detects endogenous levels of total Ki67.

RRID:

AB_2837694
Cite Format: Affinity Biosciences Cat# AF5208, RRID:AB_2837694.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Antigen identified by monoclonal antibody Ki 67; Antigen identified by monoclonal antibody Ki-67; Antigen KI-67; Antigen KI67; Antigen Ki67; KI67_HUMAN; KIA; Marker of proliferation Ki-67; MIB 1; MIB; MKI67; PPP1R105; Proliferation marker protein Ki-67; Proliferation related Ki 67 antigen; Protein phosphatase 1 regulatory subunit 105; RP11-380J17.2;

Immunogens

Immunogen:

Uniprot:

P46013(KI67_HUMAN) >>Visit HPA database.

Gene(ID):

MKI67(4288)

Description:

Interacts with KIF15. Binds through the FHA domain to NIFK. Part of a complex composed at least of ASCL2, C11orf30/EMSY, HCFC1, HSPA8, CCAR2, MATR3, MKI67, RBBP5, TUBB2A, WDR5 and ZNF335; this complex may have a histone H3-specific methyltransferase activity

Sequence:

P46013 KI67_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MWPTRRLVTIKRSGVDGPHFPLSLSTCLFGRGIECDIRIQLPVVSKQHCKIEIHEQEAILHNFSSTNPTQVNGSVIDEPVRLKHGDVITIIDRSFRYENESLQNGRKSTEFPRKIREQEPARRVSRSSFSSDPDEKAQDSKAYSKITEGKVSGNPQVHIKNVKEDSTADDSKDSVAQGTTNVHSSEHAGRNGRNAADPISGDFKEISSVKLVSRYGELKSVPTTQCLDNSKKNESPFWKLYESVKKELDVKSQKENVLQYCRKSGLQTDYATEKESADGLQGETQLLVSRKSRPKSGGSGHAVAEPASPEQELDQNKGKGRDVESVQTPSKAVGASFPLYEPAKMKTPVQYSQQQNSPQKHKNKDLYTTGRRESVNLGKSEGFKAGDKTLTPRKLSTRNRTPAKVEDAADSATKPENLSSKTRGSIPTDVEVLPTETEIHNEPFLTLWLTQVERKIQKDSLSKPEKLGTTAGQMCSGLPGLSSVDINNFGDSINESEGIPLKRRRVSFGGHLRPELFDENLPPNTPLKRGEAPTKRKSLVMHTPPVLKKIIKEQPQPSGKQESGSEIHVEVKAQSLVISPPAPSPRKTPVASDQRRRSCKTAPASSSKSQTEVPKRGGRKSGNLPSKRVSISRSQHDILQMICSKRRSGASEANLIVAKSWADVVKLGAKQTQTKVIKHGPQRSMNKRQRRPATPKKPVGEVHSQFSTGHANSPCTIIIGKAHTEKVHVPARPYRVLNNFISNQKMDFKEDLSGIAEMFKTPVKEQPQLTSTCHIAISNSENLLGKQFQGTDSGEEPLLPTSESFGGNVFFSAQNAAKQPSDKCSASPPLRRQCIRENGNVAKTPRNTYKMTSLETKTSDTETEPSKTVSTANRSGRSTEFRNIQKLPVESKSEETNTEIVECILKRGQKATLLQQRREGEMKEIERPFETYKENIELKENDEKMKAMKRSRTWGQKCAPMSDLTDLKSLPDTELMKDTARGQNLLQTQDHAKAPKSEKGKITKMPCQSLQPEPINTPTHTKQQLKASLGKVGVKEELLAVGKFTRTSGETTHTHREPAGDGKSIRTFKESPKQILDPAARVTGMKKWPRTPKEEAQSLEDLAGFKELFQTPGPSEESMTDEKTTKIACKSPPPESVDTPTSTKQWPKRSLRKADVEEEFLALRKLTPSAGKAMLTPKPAGGDEKDIKAFMGTPVQKLDLAGTLPGSKRQLQTPKEKAQALEDLAGFKELFQTPGHTEELVAAGKTTKIPCDSPQSDPVDTPTSTKQRPKRSIRKADVEGELLACRNLMPSAGKAMHTPKPSVGEEKDIIIFVGTPVQKLDLTENLTGSKRRPQTPKEEAQALEDLTGFKELFQTPGHTEEAVAAGKTTKMPCESSPPESADTPTSTRRQPKTPLEKRDVQKELSALKKLTQTSGETTHTDKVPGGEDKSINAFRETAKQKLDPAASVTGSKRHPKTKEKAQPLEDLAGLKELFQTPVCTDKPTTHEKTTKIACRSQPDPVDTPTSSKPQSKRSLRKVDVEEEFFALRKRTPSAGKAMHTPKPAVSGEKNIYAFMGTPVQKLDLTENLTGSKRRLQTPKEKAQALEDLAGFKELFQTRGHTEESMTNDKTAKVACKSSQPDPDKNPASSKRRLKTSLGKVGVKEELLAVGKLTQTSGETTHTHTEPTGDGKSMKAFMESPKQILDSAASLTGSKRQLRTPKGKSEVPEDLAGFIELFQTPSHTKESMTNEKTTKVSYRASQPDLVDTPTSSKPQPKRSLRKADTEEEFLAFRKQTPSAGKAMHTPKPAVGEEKDINTFLGTPVQKLDQPGNLPGSNRRLQTRKEKAQALEELTGFRELFQTPCTDNPTTDEKTTKKILCKSPQSDPADTPTNTKQRPKRSLKKADVEEEFLAFRKLTPSAGKAMHTPKAAVGEEKDINTFVGTPVEKLDLLGNLPGSKRRPQTPKEKAKALEDLAGFKELFQTPGHTEESMTDDKITEVSCKSPQPDPVKTPTSSKQRLKISLGKVGVKEEVLPVGKLTQTSGKTTQTHRETAGDGKSIKAFKESAKQMLDPANYGTGMERWPRTPKEEAQSLEDLAGFKELFQTPDHTEESTTDDKTTKIACKSPPPESMDTPTSTRRRPKTPLGKRDIVEELSALKQLTQTTHTDKVPGDEDKGINVFRETAKQKLDPAASVTGSKRQPRTPKGKAQPLEDLAGLKELFQTPICTDKPTTHEKTTKIACRSPQPDPVGTPTIFKPQSKRSLRKADVEEESLALRKRTPSVGKAMDTPKPAGGDEKDMKAFMGTPVQKLDLPGNLPGSKRWPQTPKEKAQALEDLAGFKELFQTPGTDKPTTDEKTTKIACKSPQPDPVDTPASTKQRPKRNLRKADVEEEFLALRKRTPSAGKAMDTPKPAVSDEKNINTFVETPVQKLDLLGNLPGSKRQPQTPKEKAEALEDLVGFKELFQTPGHTEESMTDDKITEVSCKSPQPESFKTSRSSKQRLKIPLVKVDMKEEPLAVSKLTRTSGETTQTHTEPTGDSKSIKAFKESPKQILDPAASVTGSRRQLRTRKEKARALEDLVDFKELFSAPGHTEESMTIDKNTKIPCKSPPPELTDTATSTKRCPKTRPRKEVKEELSAVERLTQTSGQSTHTHKEPASGDEGIKVLKQRAKKKPNPVEEEPSRRRPRAPKEKAQPLEDLAGFTELSETSGHTQESLTAGKATKIPCESPPLEVVDTTASTKRHLRTRVQKVQVKEEPSAVKFTQTSGETTDADKEPAGEDKGIKALKESAKQTPAPAASVTGSRRRPRAPRESAQAIEDLAGFKDPAAGHTEESMTDDKTTKIPCKSSPELEDTATSSKRRPRTRAQKVEVKEELLAVGKLTQTSGETTHTDKEPVGEGKGTKAFKQPAKRKLDAEDVIGSRRQPRAPKEKAQPLEDLASFQELSQTPGHTEELANGAADSFTSAPKQTPDSGKPLKISRRVLRAPKVEPVGDVVSTRDPVKSQSKSNTSLPPLPFKRGGGKDGSVTGTKRLRCMPAPEEIVEELPASKKQRVAPRARGKSSEPVVIMKRSLRTSAKRIEPAEELNSNDMKTNKEEHKLQDSVPENKGISLRSRRQNKTEAEQQITEVFVLAERIEINRNEKKPMKTSPEMDIQNPDDGARKPIPRDKVTENKRCLRSARQNESSQPKVAEESGGQKSAKVLMQNQKGKGEAGNSDSMCLRSRKTKSQPAASTLESKSVQRVTRSVKRCAENPKKAEDNVCVKKIRTRSHRDSEDI

PTMs - P46013 As Substrate

P46013

Site	PTM Type	Enzyme	Source
K11	Ubiquitination		Uniprot
K46	Ubiquitination		Uniprot
K83	Ubiquitination		Uniprot
R93	Methylation		Uniprot
K107	Ubiquitination		Uniprot
S108	Phosphorylation		Uniprot
T109	Phosphorylation		Uniprot
K114	Ubiquitination		Uniprot
S125	Phosphorylation		Uniprot
S127	Phosphorylation		Uniprot
S128	Phosphorylation		Uniprot
S130	Phosphorylation		Uniprot
S131	Phosphorylation		Uniprot
K136	Ubiquitination		Uniprot
K145	Ubiquitination		Uniprot
K150	Ubiquitination		Uniprot
K160	Ubiquitination		Uniprot
K163	Sumoylation		Uniprot
K163	Ubiquitination		Uniprot
K172	Ubiquitination		Uniprot
S184	Phosphorylation		Uniprot
S185	Phosphorylation		Uniprot
K204	Sumoylation		Uniprot
K204	Ubiquitination		Uniprot
K210	Ubiquitination		Uniprot
K219	Methylation		Uniprot
K219	Ubiquitination		Uniprot
K231	Ubiquitination		Uniprot
K232	Ubiquitination		Uniprot
S235	Phosphorylation		Uniprot
K239	Ubiquitination		Uniprot
Y241	Phosphorylation		Uniprot
S243	Phosphorylation		Uniprot
K245	Sumoylation		Uniprot
K245	Ubiquitination		Uniprot
K251	Ubiquitination		Uniprot
K254	Ubiquitination		Uniprot
K263	Ubiquitination		Uniprot
S264	Phosphorylation		Uniprot
T268	Phosphorylation		Uniprot
K274	Ubiquitination		Uniprot
S296	Phosphorylation		Uniprot
S299	Phosphorylation		Uniprot
S308	Phosphorylation		Uniprot
K317	Ubiquitination		Uniprot
S325	Phosphorylation		Uniprot
T328	Phosphorylation		Uniprot
S330	Phosphorylation		Uniprot
K331	Ubiquitination		Uniprot
S336	Phosphorylation		Uniprot
Y340	Phosphorylation		Uniprot
K344	Ubiquitination		Uniprot
K346	Ubiquitination		Uniprot
T347	Phosphorylation		Uniprot
S352	Phosphorylation		Uniprot
S357	Phosphorylation	P24941 (CDK2)	Uniprot
K360	Ubiquitination		Uniprot
K364	Ubiquitination		Uniprot
Y367	Phosphorylation		Uniprot
T368	Phosphorylation		Uniprot
S374	Phosphorylation	Q96GD4 (AURKB)	Uniprot
K379	Acetylation		Uniprot
K379	Ubiquitination		Uniprot
K388	Acetylation		Uniprot
T389	Phosphorylation		Uniprot
T391	Phosphorylation		Uniprot
S396	Phosphorylation		Uniprot
T401	Phosphorylation		Uniprot
S411	Phosphorylation		Uniprot
T413	Phosphorylation		Uniprot
K414	Ubiquitination		Uniprot
K421	Ubiquitination		Uniprot
S425	Phosphorylation		Uniprot
K458	Ubiquitination		Uniprot
S460	Phosphorylation		Uniprot
K466	Acetylation		Uniprot
K502	Methylation		Uniprot
K502	Ubiquitination		Uniprot
S507	Phosphorylation		Uniprot
T525	Phosphorylation		Uniprot
K528	Ubiquitination		Uniprot
K537	Ubiquitination		Uniprot
S538	Phosphorylation	Q96GD4 (AURKB)	Uniprot
T543	Phosphorylation		Uniprot
K548	Ubiquitination		Uniprot
K552	Sumoylation		Uniprot
K552	Ubiquitination		Uniprot
K560	Ubiquitination		Uniprot
S563	Phosphorylation		Uniprot
S565	Phosphorylation		Uniprot
S575	Phosphorylation		Uniprot
S579	Phosphorylation		Uniprot
S584	Phosphorylation		Uniprot
K587	Sumoylation		Uniprot
K587	Ubiquitination		Uniprot
T588	Phosphorylation		Uniprot
S592	Phosphorylation		Uniprot
S598	Phosphorylation		Uniprot
T601	Phosphorylation		Uniprot
S605	Phosphorylation		Uniprot
K608	Ubiquitination		Uniprot
T611	Phosphorylation		Uniprot
K615	Ubiquitination		Uniprot
K620	Ubiquitination		Uniprot
S621	Phosphorylation		Uniprot
K627	Ubiquitination		Uniprot
S634	Phosphorylation		Uniprot
K645	Ubiquitination		Uniprot
S648	Phosphorylation		Uniprot
S651	Phosphorylation		Uniprot
K659	Ubiquitination		Uniprot
S660	Phosphorylation		Uniprot
K666	Ubiquitination		Uniprot
K670	Ubiquitination		Uniprot
K675	Ubiquitination		Uniprot
T694	Phosphorylation		Uniprot
K696	Ubiquitination		Uniprot
K697	Ubiquitination		Uniprot
S704	Phosphorylation		Uniprot
S707	Phosphorylation		Uniprot
S713	Phosphorylation		Uniprot
T716	Phosphorylation		Uniprot
K721	Ubiquitination		Uniprot
K726	Ubiquitination		Uniprot
K745	Sumoylation		Uniprot
K745	Ubiquitination		Uniprot
K749	Sumoylation		Uniprot
K749	Ubiquitination		Uniprot
S753	Phosphorylation		Uniprot
K760	Ubiquitination		Uniprot
T761	Phosphorylation	P06493 (CDK1)	Uniprot
K764	Ubiquitination		Uniprot
K818	Ubiquitination		Uniprot
S825	Phosphorylation		Uniprot
S827	Phosphorylation		Uniprot
T844	Phosphorylation		Uniprot
Y849	Phosphorylation		Uniprot
K850	Ubiquitination		Uniprot
S853	Phosphorylation		Uniprot
K857	Ubiquitination		Uniprot
T858	Phosphorylation		Uniprot
S859	Phosphorylation		Uniprot
K867	Ubiquitination		Uniprot
S878	Phosphorylation		Uniprot
K886	Ubiquitination		Uniprot
K892	Sumoylation		Uniprot
K892	Ubiquitination		Uniprot
K906	Ubiquitination		Uniprot
K910	Ubiquitination		Uniprot
K923	Ubiquitination		Uniprot
K933	Ubiquitination		Uniprot
K957	Ubiquitination		Uniprot
K968	Ubiquitination		Uniprot
S969	Phosphorylation		Uniprot
K977	Ubiquitination		Uniprot
T988	Phosphorylation		Uniprot
K993	Ubiquitination		Uniprot
K1004	Ubiquitination		Uniprot
T1017	Phosphorylation		Uniprot
K1022	Ubiquitination		Uniprot
K1026	Ubiquitination		Uniprot
S1028	Phosphorylation		Uniprot
K1031	Ubiquitination		Uniprot
K1035	Sumoylation		Uniprot
K1035	Ubiquitination		Uniprot
K1043	Ubiquitination		Uniprot
K1063	Acetylation		Uniprot
K1063	Ubiquitination		Uniprot
T1067	Phosphorylation		Uniprot
K1069	Sumoylation		Uniprot
S1071	Phosphorylation		Uniprot
K1073	Ubiquitination		Uniprot
T1091	Phosphorylation		Uniprot
K1093	Sumoylation		Uniprot
K1093	Ubiquitination		Uniprot
S1098	Phosphorylation		Uniprot
K1106	Ubiquitination		Uniprot
T1111	Phosphorylation		Uniprot
S1115	Phosphorylation		Uniprot
S1118	Phosphorylation		Uniprot
S1131	Phosphorylation		Uniprot
S1136	Phosphorylation		Uniprot
T1139	Phosphorylation		Uniprot
T1141	Phosphorylation		Uniprot
S1142	Phosphorylation		Uniprot
T1143	Phosphorylation		Uniprot
K1144	Ubiquitination		Uniprot
K1153	Sumoylation		Uniprot
K1153	Ubiquitination		Uniprot
K1165	Acetylation		Uniprot
K1165	Ubiquitination		Uniprot
T1167	Phosphorylation		Uniprot
S1169	Phosphorylation		Uniprot
K1172	Ubiquitination		Uniprot
T1176	Phosphorylation		Uniprot
K1178	Acetylation		Uniprot
K1185	Acetylation		Uniprot
K1188	Sumoylation		Uniprot
K1188	Ubiquitination		Uniprot
T1193	Phosphorylation		Uniprot
K1197	Ubiquitination		Uniprot
T1203	Phosphorylation		Uniprot
S1207	Phosphorylation		Uniprot
K1208	Acetylation		Uniprot
K1208	Ubiquitination		Uniprot
T1213	Phosphorylation		Uniprot
K1217	Ubiquitination		Uniprot
K1228	Ubiquitination		Uniprot
T1233	Phosphorylation		Uniprot
T1237	Phosphorylation		Uniprot
K1245	Ubiquitination		Uniprot
K1248	Ubiquitination		Uniprot
C1251	S-Nitrosylation		Uniprot
S1253	Phosphorylation		Uniprot
S1256	Phosphorylation		Uniprot
T1261	Phosphorylation		Uniprot
T1263	Phosphorylation		Uniprot
S1264	Phosphorylation		Uniprot
T1265	Phosphorylation		Uniprot
K1266	Ubiquitination		Uniprot
S1272	Phosphorylation		Uniprot
K1275	Ubiquitination		Uniprot
S1291	Phosphorylation		Uniprot
T1298	Phosphorylation		Uniprot
S1302	Phosphorylation		Uniprot
T1315	Phosphorylation		Uniprot
T1323	Phosphorylation		Uniprot
T1327	Phosphorylation		Uniprot
S1329	Phosphorylation		Uniprot
K1330	Acetylation		Uniprot
K1330	Ubiquitination		Uniprot
T1335	Phosphorylation		Uniprot
K1337	Ubiquitination		Uniprot
T1347	Phosphorylation		Uniprot
K1350	Ubiquitination		Uniprot
T1355	Phosphorylation		Uniprot
T1359	Phosphorylation		Uniprot
K1367	Ubiquitination		Uniprot
S1375	Phosphorylation		Uniprot
S1376	Phosphorylation		Uniprot
S1380	Phosphorylation		Uniprot
T1383	Phosphorylation		Uniprot
T1385	Phosphorylation		Uniprot
S1386	Phosphorylation		Uniprot
T1387	Phosphorylation		Uniprot
T1393	Phosphorylation		Uniprot
K1402	Ubiquitination		Uniprot
S1405	Phosphorylation		Uniprot
K1408	Acetylation		Uniprot
K1409	Ubiquitination		Uniprot
T1411	Phosphorylation		Uniprot
S1414	Phosphorylation		Uniprot
K1422	Ubiquitination		Uniprot
K1441	Ubiquitination		Uniprot
S1447	Phosphorylation		Uniprot
T1449	Phosphorylation		Uniprot
S1451	Phosphorylation		Uniprot
K1452	Acetylation		Uniprot
K1452	Ubiquitination		Uniprot
K1460	Ubiquitination		Uniprot
K1471	Ubiquitination		Uniprot
T1476	Phosphorylation		Uniprot
T1480	Phosphorylation		Uniprot
K1482	Acetylation		Uniprot
K1482	Ubiquitination		Uniprot
T1485	Phosphorylation		Uniprot
S1496	Phosphorylation		Uniprot
T1503	Phosphorylation		Uniprot
T1505	Phosphorylation		Uniprot
S1506	Phosphorylation		Uniprot
S1507	Phosphorylation		Uniprot
K1508	Ubiquitination		Uniprot
S1511	Phosphorylation		Uniprot
K1512	Methylation		Uniprot
K1512	Ubiquitination		Uniprot
S1514	Phosphorylation		Uniprot
K1517	Sumoylation		Uniprot
K1517	Ubiquitination		Uniprot
T1531	Phosphorylation		Uniprot
S1533	Phosphorylation		Uniprot
T1540	Phosphorylation		Uniprot
K1542	Ubiquitination		Uniprot
S1546	Phosphorylation		Uniprot
K1549	Ubiquitination		Uniprot
Y1552	Phosphorylation		Uniprot
T1557	Phosphorylation		Uniprot
T1565	Phosphorylation		Uniprot
T1569	Phosphorylation		Uniprot
S1571	Phosphorylation		Uniprot
K1572	Acetylation		Uniprot
K1572	Ubiquitination		Uniprot
T1577	Phosphorylation		Uniprot
K1581	Acetylation		Uniprot
K1581	Ubiquitination		Uniprot
K1592	Acetylation		Uniprot
K1592	Ubiquitination		Uniprot
T1597	Phosphorylation		Uniprot
T1601	Phosphorylation		Uniprot
S1604	Phosphorylation		Uniprot
K1612	Acetylation		Uniprot
S1618	Phosphorylation		Uniprot
S1628	Phosphorylation		Uniprot
S1629	Phosphorylation		Uniprot
S1636	Phosphorylation		Uniprot
K1639	Acetylation		Uniprot
K1639	Ubiquitination		Uniprot
K1643	Sumoylation		Uniprot
K1643	Ubiquitination		Uniprot
K1651	Ubiquitination		Uniprot
S1656	Phosphorylation		Uniprot
T1660	Phosphorylation		Uniprot
S1679	Phosphorylation		Uniprot
S1686	Phosphorylation		Uniprot
S1689	Phosphorylation		Uniprot
T1691	Phosphorylation		Uniprot
S1693	Phosphorylation		Uniprot
K1694	Ubiquitination		Uniprot
T1699	Phosphorylation		Uniprot
K1703	Sumoylation		Uniprot
K1703	Ubiquitination		Uniprot
S1704	Phosphorylation		Uniprot
T1719	Phosphorylation		Uniprot
S1721	Phosphorylation		Uniprot
K1724	Ubiquitination		Uniprot
S1726	Phosphorylation		Uniprot
K1734	Ubiquitination		Uniprot
S1736	Phosphorylation		Uniprot
Y1737	Phosphorylation		Uniprot
S1740	Phosphorylation		Uniprot
T1747	Phosphorylation		Uniprot
T1749	Phosphorylation		Uniprot
S1750	Phosphorylation		Uniprot
S1751	Phosphorylation		Uniprot
K1752	Ubiquitination		Uniprot
S1758	Phosphorylation		Uniprot
K1761	Ubiquitination		Uniprot
T1764	Phosphorylation	O14757 (CHEK1)	Uniprot
T1775	Phosphorylation		Uniprot
S1777	Phosphorylation		Uniprot
T1784	Phosphorylation		Uniprot
K1793	Ubiquitination		Uniprot
T1797	Phosphorylation		Uniprot
T1801	Phosphorylation		Uniprot
K1805	Ubiquitination		Uniprot
S1815	Phosphorylation		Uniprot
K1825	Ubiquitination		Uniprot
T1841	Phosphorylation		Uniprot
T1844	Phosphorylation		Uniprot
T1848	Phosphorylation		Uniprot
T1849	Phosphorylation		Uniprot
K1852	Ubiquitination		Uniprot
T1853	Phosphorylation		Uniprot
K1860	Methylation		Uniprot
K1860	Ubiquitination		Uniprot
S1861	Phosphorylation		Uniprot
S1864	Phosphorylation		Uniprot
T1869	Phosphorylation		Uniprot
T1871	Phosphorylation		Uniprot
K1874	Ubiquitination		Uniprot
S1880	Phosphorylation		Uniprot
K1883	Sumoylation		Uniprot
K1883	Ubiquitination		Uniprot
K1895	Ubiquitination		Uniprot
T1897	Phosphorylation		Uniprot
S1899	Phosphorylation		Uniprot
K1902	Acetylation		Uniprot
K1902	Ubiquitination		Uniprot
T1906	Phosphorylation		Uniprot
K1915	Ubiquitination		Uniprot
T1919	Phosphorylation		Uniprot
T1923	Phosphorylation		Uniprot
K1927	Ubiquitination		Uniprot
S1937	Phosphorylation		Uniprot
K1938	Acetylation		Uniprot
K1938	Ubiquitination		Uniprot
T1943	Phosphorylation	P06493 (CDK1)	Uniprot
K1949	Ubiquitination		Uniprot
K1958	Ubiquitination		Uniprot
T1963	Phosphorylation		Uniprot
T1967	Phosphorylation		Uniprot
S1970	Phosphorylation		Uniprot
S1980	Phosphorylation		Uniprot
K1982	Ubiquitination		Uniprot
S1983	Phosphorylation		Uniprot
T1991	Phosphorylation		Uniprot
S2002	Phosphorylation		Uniprot
K2005	Acetylation		Uniprot
K2005	Ubiquitination		Uniprot
K2009	Sumoylation		Uniprot
K2009	Ubiquitination		Uniprot
T2019	Phosphorylation		Uniprot
T2021	Phosphorylation		Uniprot
T2025	Phosphorylation		Uniprot
T2026	Phosphorylation		Uniprot
T2028	Phosphorylation		Uniprot
T2065	Phosphorylation		Uniprot
K2067	Sumoylation		Uniprot
K2067	Ubiquitination		Uniprot
S2072	Phosphorylation		Uniprot
K2080	Ubiquitination		Uniprot
T2085	Phosphorylation		Uniprot
T2089	Phosphorylation		Uniprot
S2105	Phosphorylation		Uniprot
S2110	Phosphorylation		Uniprot
T2113	Phosphorylation		Uniprot
T2115	Phosphorylation		Uniprot
S2116	Phosphorylation		Uniprot
T2123	Phosphorylation		Uniprot
S2135	Phosphorylation		Uniprot
K2138	Ubiquitination		Uniprot
K2167	Ubiquitination		Uniprot
S2173	Phosphorylation		Uniprot
T2175	Phosphorylation		Uniprot
K2178	Acetylation		Uniprot
K2178	Ubiquitination		Uniprot
K2187	Ubiquitination		Uniprot
K2198	Ubiquitination		Uniprot
T2203	Phosphorylation		Uniprot
T2207	Phosphorylation		Uniprot
T2211	Phosphorylation		Uniprot
T2212	Phosphorylation		Uniprot
T2217	Phosphorylation		Uniprot
S2223	Phosphorylation		Uniprot
T2231	Phosphorylation		Uniprot
T2233	Phosphorylation		Uniprot
K2236	Ubiquitination		Uniprot
S2239	Phosphorylation		Uniprot
K2240	Methylation		Uniprot
S2242	Phosphorylation		Uniprot
K2245	Ubiquitination		Uniprot
S2252	Phosphorylation		Uniprot
T2259	Phosphorylation		Uniprot
S2261	Phosphorylation		Uniprot
K2264	Acetylation		Uniprot
T2268	Phosphorylation		Uniprot
K2280	Acetylation		Uniprot
K2280	Sumoylation		Uniprot
T2285	Phosphorylation		Uniprot
K2289	Acetylation		Uniprot
K2289	Ubiquitination		Uniprot
S2299	Phosphorylation		Uniprot
K2300	Acetylation		Uniprot
K2300	Ubiquitination		Uniprot
T2305	Phosphorylation		Uniprot
K2309	Ubiquitination		Uniprot
K2320	Ubiquitination		Uniprot
T2325	Phosphorylation		Uniprot
T2328	Phosphorylation		Uniprot
K2330	Ubiquitination		Uniprot
T2332	Phosphorylation		Uniprot
T2333	Phosphorylation		Uniprot
T2337	Phosphorylation		Uniprot
K2343	Methylation		Uniprot
K2343	Ubiquitination		Uniprot
S2344	Phosphorylation		Uniprot
T2352	Phosphorylation		Uniprot
S2355	Phosphorylation		Uniprot
T2356	Phosphorylation		Uniprot
K2366	Sumoylation		Uniprot
K2366	Ubiquitination		Uniprot
T2380	Phosphorylation		Uniprot
S2382	Phosphorylation		Uniprot
T2389	Phosphorylation		Uniprot
S2395	Phosphorylation		Uniprot
K2398	Sumoylation		Uniprot
K2398	Ubiquitination		Uniprot
T2402	Phosphorylation		Uniprot
T2406	Phosphorylation		Uniprot
K2410	Ubiquitination		Uniprot
S2420	Phosphorylation		Uniprot
K2421	Acetylation		Uniprot
K2421	Ubiquitination		Uniprot
T2426	Phosphorylation		Uniprot
K2430	Sumoylation		Uniprot
K2430	Ubiquitination		Uniprot
K2441	Ubiquitination		Uniprot
T2446	Phosphorylation		Uniprot
T2450	Phosphorylation		Uniprot
S2453	Phosphorylation		Uniprot
T2455	Phosphorylation		Uniprot
T2460	Phosphorylation		Uniprot
S2463	Phosphorylation		Uniprot
S2466	Phosphorylation		Uniprot
S2471	Phosphorylation		Uniprot
K2473	Methylation		Uniprot
K2488	Ubiquitination		Uniprot
K2492	Sumoylation		Uniprot
K2500	Ubiquitination		Uniprot
T2502	Phosphorylation		Uniprot
T2504	Phosphorylation		Uniprot
S2505	Phosphorylation		Uniprot
T2508	Phosphorylation		Uniprot
T2509	Phosphorylation		Uniprot
T2516	Phosphorylation		Uniprot
K2520	Acetylation		Uniprot
K2523	Acetylation		Uniprot
S2528	Phosphorylation		Uniprot
K2530	Ubiquitination		Uniprot
S2538	Phosphorylation		Uniprot
S2542	Phosphorylation		Uniprot
K2587	Ubiquitination		Uniprot
S2588	Phosphorylation		Uniprot
T2594	Phosphorylation		Uniprot
T2598	Phosphorylation		Uniprot
T2600	Phosphorylation		Uniprot
K2601	Ubiquitination		Uniprot
K2613	Sumoylation		Uniprot
K2613	Ubiquitination		Uniprot
K2634	Ubiquitination		Uniprot
S2638	Phosphorylation		Uniprot
T2683	Phosphorylation		Uniprot
T2692	Phosphorylation		Uniprot
S2695	Phosphorylation		Uniprot
T2697	Phosphorylation		Uniprot
S2708	Phosphorylation		Uniprot
T2716	Phosphorylation		Uniprot
T2717	Phosphorylation		Uniprot
S2719	Phosphorylation		Uniprot
T2720	Phosphorylation		Uniprot
K2734	Sumoylation		Uniprot
T2749	Phosphorylation		Uniprot
T2750	Phosphorylation		Uniprot
K2767	Ubiquitination		Uniprot
S2769	Phosphorylation		Uniprot
T2773	Phosphorylation		Uniprot
T2781	Phosphorylation		Uniprot
S2783	Phosphorylation		Uniprot
S2793	Phosphorylation		Uniprot
S2814	Phosphorylation		Uniprot
T2816	Phosphorylation		Uniprot
S2827	Phosphorylation		Uniprot
S2828	Phosphorylation		Uniprot
T2834	Phosphorylation		Uniprot
T2836	Phosphorylation		Uniprot
S2837	Phosphorylation		Uniprot
S2838	Phosphorylation		Uniprot
K2852	Sumoylation		Uniprot
K2852	Ubiquitination		Uniprot
T2862	Phosphorylation		Uniprot
K2892	Ubiquitination		Uniprot
S2901	Phosphorylation		Uniprot
S2920	Phosphorylation		Uniprot
S2925	Phosphorylation		Uniprot
T2927	Phosphorylation		Uniprot
T2931	Phosphorylation		Uniprot
S2941	Phosphorylation		Uniprot
T2949	Phosphorylation		Uniprot
S2952	Phosphorylation		Uniprot
K2967	Ubiquitination		Uniprot
K2986	Acetylation		Uniprot
K2997	Acetylation		Uniprot
K2997	Methylation		Uniprot
T3007	Phosphorylation		Uniprot
T3009	Phosphorylation		Uniprot
S3041	Phosphorylation		Uniprot
S3042	Phosphorylation		Uniprot
S3067	Phosphorylation		Uniprot
S3082	Phosphorylation		Uniprot
K3087	Ubiquitination		Uniprot
T3127	Phosphorylation		Uniprot
S3128	Phosphorylation		Uniprot
K3180	Ubiquitination		Uniprot
S3197	Phosphorylation		Uniprot
T3213	Phosphorylation		Uniprot
S3216	Phosphorylation		Uniprot
K3217	Ubiquitination		Uniprot
S3218	Phosphorylation		Uniprot
T3223	Phosphorylation		Uniprot
S3249	Phosphorylation		Uniprot
S3253	Phosphorylation		Uniprot

Research Backgrounds

P46013_KI67_HUMAN

Function:

Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly. Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface. Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility. Binds DNA, with a preference for supercoiled DNA and AT-rich DNA. Does not contribute to the internal structure of mitotic chromosomes (By similarity). May play a role in chromatin organization. It is however unclear whether it plays a direct role in chromatin organization or whether it is an indirect consequence of its function in maintaining mitotic chromosomes dispersed (Probable).

PTMs:

Phosphorylated. Hyperphosphorylated in mitosis. Hyperphosphorylated form does not bind DNA.

Subcellular Location:

Chromosome. Nucleus. Nucleus>Nucleolus.
Note: Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the mitotic chromosome surface (PubMed:27362226). Associates with satellite DNA in G1 phase (PubMed:9510506). Binds tightly to chromatin in interphase, chromatin-binding decreases in mitosis when it associates with the surface of the condensed chromosomes (PubMed:15896774, PubMed:22002106). Predominantly localized in the G1 phase in the perinucleolar region, in the later phases it is also detected throughout the nuclear interior, being predominantly localized in the nuclear matrix (PubMed:22002106).

Subunit Structure:

Interacts with KIF15. Interacts (via the FHA domain) with NIFK. Interacts with PPP1CC. Part of a complex composed at least of ASCL2, EMSY, HCFC1, HSPA8, CCAR2, MATR3, MKI67, RBBP5, TUBB2A, WDR5 and ZNF335; this complex may have a histone H3-specific methyltransferase activity.

References

1). Organoids From Mucinous Appendiceal Adenocarcinomas as High-Fidelity Models for Individual Therapy. Frontiers in Medicine (PubMed: 35721089) [IF=3.9]

Application: IHC Species: Human Sample: tumor tissues

Figure 2 Representative images of the pathological staining of MAA. (A) H&E staining of the organoid (passage 8) and the original tumor. (B) Immunohistochemistry staining of the original tumor and the organoid (passage 8) for Ki67 and PDL-1. (C) Immunohistochemistry staining of the organoid (passage 8) for CD44.

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Ki67 Antibody - #AF5208