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$280 100ul
$350 200ul

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  • Product Name
    HOXB5 Antibody
  • Catalog No.
    AF5215
  • Source
    Rabbit
  • Application
    WB,ELISA
  • Reactivity:
    Human, Mouse
  • Prediction:
    Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(85%)
  • UniProt
  • Mol.Wt.
    29 kDa
  • Concentration
    1mg/ml
  • Browse similar products>>

Product Information

Alternative Names:Expand▼

HGNC:5116; HHO.C10; Homeo box 2A; Homeo box B5; Homeobox 2A; Homeobox B5; Homeobox protein HHO.C10; Homeobox protein Hox B5; Homeobox protein Hox-2A; Homeobox protein Hox-B5; Homeobox protein HoxB5; Homeobox protein Hu-1; Homeobox2A; HomeoboxB5; HOX 2; Hox 2.1; HOX 2A; HOX B5; HOX2; Hox2.1; Hox2.1, mouse, homolog of; HOX2A; HOXB 5; Hoxb5; HU 1; HU1; HXB5_HUMAN;

Applications:

WB 1:500-1:2000, ELISA(peptide) 1:20000-1:40000

Reactivity:

Human, Mouse

Predicted Reactivity:

Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(85%)

Source:

Rabbit

Clonality:

Polyclonal

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Specificity:

HOXB5 Antibody detects endogenous levels of total HOXB5.

Format:

Liquid

Concentration:

1mg/ml

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

Immunogen Information

Immunogen:

A synthesized peptide derived from human HOXB5, corresponding to a region within the internal amino acids.

Uniprot:



>>Visit The Human Protein Atlas

Gene id:

Molecular Weight:

Observed Mol.Wt.: 29 kDa.
Predicted Mol.Wt.: 30kDa.

Subcellular Location:

Nucleus.

Tissue Specificity:

Spinal cord.

Description:

Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.

Sequence:
        10         20         30         40         50
MSSYFVNSFS GRYPNGPDYQ LLNYGSGSSL SGSYRDPAAM HTGSYGYNYN
60 70 80 90 100
GMDLSVNRSS ASSSHFGAVG ESSRAFPAPA QEPRFRQAAS SCSLSSPESL
110 120 130 140 150
PCTNGDSHGA KPSASSPSDQ ATSASSSANF TEIDEASASS EPEEAASQLS
160 170 180 190 200
SPSLARAQPE PMATSTAAPE GQTPQIFPWM RKLHISHDMT GPDGKRARTA
210 220 230 240 250
YTRYQTLELE KEFHFNRYLT RRRRIEIAHA LCLSERQIKI WFQNRRMKWK
260
KDNKLKSMSL ATAGSAFQP

Background

Function:

Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.

Subcellular Location:

Nucleus;

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte

Similarity:

Belongs to the Antp homeobox family.

Western blot analysis of HOXB5 expression in HUVEC cells,The lane on the left was treated with the antigen-specific peptide.
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Catalog Number :

AF5215-BP

Price/Size :

$200/1mg.
Tips: For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Function :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

Format and storage :

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.

Precautions :

This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Chicken
100%
Rabbit
100%
Pig
100%
Dog
100%
Sheep
100%
Horse
100%
Bovine
100%
Xenopus
85%
Zebrafish
0%
High similarity Medium similarity Low similarity No similarity
P09067 as Substrate
Site PTM Type Enzyme
S55 Phosphorylation
S186 Phosphorylation
T199 Phosphorylation
Y201 Phosphorylation
T202 Phosphorylation
K211 Ubiquitination
R217 Methylation
S257 Phosphorylation
S259 Phosphorylation
IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.