SOX6 Antibody - #AF5217

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Product: SOX6 Antibody
Catalog: AF5217
Description: Rabbit polyclonal antibody to SOX6
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 93 kDa; 92kD(Calculated).
Uniprot: P35712
RRID: AB_2837703

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
IF/ICC 1:100-1:500, WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
SOX6 Antibody detects endogenous levels of total SOX6.
RRID:
AB_2837703
Cite Format: Affinity Biosciences Cat# AF5217, RRID:AB_2837703.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

HSSOX 6; HSSOX6; SOX 6; sox6; SOX6_HUMAN; SOXD; SRY (sex determining region Y) box 6; SRY box 6; SRY box containing gene 6; Transcription factor SOX 6; Transcription factor Sox-6; Transcription factor SOX6;

Immunogens

Immunogen:
Uniprot:

P35712(SOX6_HUMAN) >>Visit HPA database.

Gene(ID):
SOX6(55553)
Expression:
P35712 SOX6_HUMAN:

Expressed in a wide variety of tissues, most abundantly in skeletal muscle.

Description:
Transcriptional activator. Binds specifically to the DNA sequence 5'-AACAAT-3'. Plays a key role in several developmental processes, including neurogenesis and skeleton formation.
Sequence:
MSSKQATSPFACAADGEDAMTQDLTSREKEEGSDQHVASHLPLHPIMHNKPHSEELPTLVSTIQQDADWDSVLSSQQRMESENNKLCSLYSFRNTSTSPHKPDEGSRDREIMTSVTFGTPERRKGSLADVVDTLKQKKLEEMTRTEQEDSSCMEKLLSKDWKEKMERLNTSELLGEIKGTPESLAEKERQLSTMITQLISLREQLLAAHDEQKKLAASQIEKQRQQMDLARQQQEQIARQQQQLLQQQHKINLLQQQIQVQGHMPPLMIPIFPHDQRTLAAAAAAQQGFLFPPGITYKPGDNYPVQFIPSTMAAAAASGLSPLQLQKGHVSHPQINQRLKGLSDRFGRNLDTFEHGGGHSYNHKQIEQLYAAQLASMQVSPGAKMPSTPQPPNTAGTVSPTGIKNEKRGTSPVTQVKDEAAAQPLNLSSRPKTAEPVKSPTSPTQNLFPASKTSPVNLPNKSSIPSPIGGSLGRGSSLDILSSLNSPALFGDQDTVMKAIQEARKMREQIQREQQQQQPHGVDGKLSSINNMGLNSCRNEKERTRFENLGPQLTGKSNEDGKLGPGVIDLTRPEDAEGSKAMNGSAAKLQQYYCWPTGGATVAEARVYRDARGRASSEPHIKRPMNAFMVWAKDERRKILQAFPDMHNSNISKILGSRWKSMSNQEKQPYYEEQARLSKIHLEKYPNYKYKPRPKRTCIVDGKKLRIGEYKQLMRSRRQEMRQFFTVGQQPQIPITTGTGVVYPGAITMATTTPSPQMTSDCSSTSASPEPSLPVIQSTYGMKTDGGSLAGNEMINGEDEMEMYDDYEDDPKSDYSSENEAPEAVSAN

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Chicken
70
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P35712 As Substrate

Site PTM Type Enzyme
S8 Phosphorylation
T21 Phosphorylation
T25 Phosphorylation
S98 Phosphorylation
S106 Phosphorylation
T119 Phosphorylation
S126 Phosphorylation
K159 Ubiquitination
S171 Phosphorylation
K340 Ubiquitination
S380 Phosphorylation
S399 Phosphorylation
K404 Sumoylation
S411 Phosphorylation
K417 Sumoylation
S439 Phosphorylation
S442 Phosphorylation
T444 Phosphorylation
S454 Phosphorylation
S466 Phosphorylation
R474 Methylation
S476 Phosphorylation
S477 Phosphorylation
S486 Phosphorylation
Y671 Phosphorylation
S678 Phosphorylation
T697 Phosphorylation
S716 Phosphorylation
T759 Phosphorylation

Research Backgrounds

Function:

Transcriptional activator. Binds specifically to the DNA sequence 5'-AACAAT-3'. Plays a key role in several developmental processes, including neurogenesis and skeleton formation.

PTMs:

Sumoylation inhibits the transcriptional activity.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in a wide variety of tissues, most abundantly in skeletal muscle.

Subunit Structure:

Interacts with DAZAP2.

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