Product: BUB3 Antibody
Catalog: AF5252
Description: Rabbit polyclonal antibody to BUB3
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 37 kDa; 37kD(Calculated).
Uniprot: O43684
RRID: AB_2837738

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
BUB3 Antibody detects endogenous levels of total BUB3.
RRID:
AB_2837738
Cite Format: Affinity Biosciences Cat# AF5252, RRID:AB_2837738.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Aa2-050; AU019800; AU021329; AU043350; AW146323; BUB 3; BUB 3L; BUB3 (budding uninhibited by benzimidazoles 3, yeast) homolog; bub3; BUB3 budding uninhibited by benzimidazoles 3 homolog; BUB3 mitotic checkpoint protein; BUB3_HUMAN; BUB3L; Budding uninhibited by benomyl; Budding uninhibited by benzimidazoles 3; Budding uninhibited by benzimidazoles 3 homolog (S. cerevisiae); Budding uninhibited by benzimidazoles 3 homolog (yeast); Budding uninhibited by benzimidazoles 3 homolog; Budding uninhibited by benzimidazoles 3, S. cerevisiae, homolog of; C78067; hBUB3; Mitotic checkpoint component; Mitotic checkpoint protein bub3; OTTHUMP00000020679; OTTHUMP00000020680; WD repeat type I transmembrane protein A72.5;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Description:
FunctionHas a dual function in spindle-assembly checkpoint signaling and in promoting the establishment of correct kinetochore-microtubule (K-MT) attachments. Promotes the formation of stable end-on bipolar attachments. Necessary for kinetochore localization of BUB1.
Sequence:
MTGSNEFKLNQPPEDGISSVKFSPNTSQFLLVSSWDTSVRLYDVPANSMRLKYQHTGAVLDCAFYDPTHAWSGGLDHQLKMHDLNTDQENLVGTHDAPIRCVEYCPEVNVMVTGSWDQTVKLWDPRTPCNAGTFSQPEKVYTLSVSGDRLIVGTAGRRVLVWDLRNMGYVQQRRESSLKYQTRCIRAFPNKQGYVLSSIEGRVAVEYLDPSPEVQKKKYAFKCHRLKENNIEQIYPVNAISFHNIHNTFATGGSDGFVNIWDPFNKKRLCQFHRYPTSIASLAFSNDGTTLAIASSYMYEMDDTEHPEDGIFIRQVTDAETKPKSPCT

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Zebrafish
100
Chicken
100
Rabbit
100
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O43684 As Substrate

Site PTM Type Enzyme
K8 Sumoylation
K8 Ubiquitination
S18 Phosphorylation PR:O43683 (hBUB1)
S19 Phosphorylation O43683 (BUB1)
K21 Ubiquitination
S33 Phosphorylation
S48 Phosphorylation
K52 Ubiquitination
K80 Ubiquitination
T86 Phosphorylation
R126 Methylation
T127 Phosphorylation
C129 S-Nitrosylation
K139 Ubiquitination
Y141 Phosphorylation
S144 Phosphorylation
R149 Methylation
T154 Phosphorylation
K179 Acetylation
K179 Ubiquitination
R186 Methylation
K191 Ubiquitination
Y194 Phosphorylation
Y207 Phosphorylation P14618 (PKM)
S211 Phosphorylation
K216 Acetylation
K216 Ubiquitination
K217 Ubiquitination
K218 Ubiquitination
K222 Ubiquitination
K227 Sumoylation
K227 Ubiquitination
K266 Ubiquitination
K322 Acetylation
K322 Sumoylation
K322 Ubiquitination
K324 Ubiquitination
S325 Phosphorylation
C327 S-Nitrosylation
T328 Phosphorylation

Research Backgrounds

Function:

Has a dual function in spindle-assembly checkpoint signaling and in promoting the establishment of correct kinetochore-microtubule (K-MT) attachments. Promotes the formation of stable end-on bipolar attachments. Necessary for kinetochore localization of BUB1. Regulates chromosome segregation during oocyte meiosis. The BUB1/BUB3 complex plays a role in the inhibition of anaphase-promoting complex or cyclosome (APC/C) when spindle-assembly checkpoint is activated and inhibits the ubiquitin ligase activity of APC/C by phosphorylating its activator CDC20. This complex can also phosphorylate MAD1L1.

PTMs:

Poly-ADP-ribosylated by PARP1.

Subcellular Location:

Nucleus. Chromosome>Centromere>Kinetochore.
Note: Starts to localize at kinetochores in prometaphase I (Pro-MI) stage and maintains the localization until the metaphase I-anaphase I (MI-AI) transition.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Interacts with BUB1 and BUBR1. The BUB1/BUB3 complex interacts with MAD1L1. Interacts with ZNF207/BuGZ; leading to promote stability and kinetochore loading of BUB3.

Family&Domains:

Belongs to the WD repeat BUB3 family.

Research Fields

· Cellular Processes > Cell growth and death > Cell cycle.   (View pathway)

· Human Diseases > Infectious diseases: Viral > HTLV-I infection.

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