Product: DUSP1/MKP1 Antibody
Catalog: AF5286
Description: Rabbit polyclonal antibody to DUSP1/MKP1
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 39 kDa; 39kD(Calculated).
Uniprot: P28562
RRID: AB_2837772

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 100ul $280 In stock
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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(88%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(88%)
Clonality:
Polyclonal
Specificity:
DUSP1/MKP1 Antibody detects endogenous levels of total DUSP1/MKP1.
RRID:
AB_2837772
Cite Format: Affinity Biosciences Cat# AF5286, RRID:AB_2837772.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CL 100; CL100; Dual Specificity Phosphatase 1; Dual specificity protein phosphatase 1; Dual specificity protein phosphatase hVH1; DUS1_HUMAN; DUSP 1; Dusp1; HVH1; MAP kinase phosphatase 1; Mitogen-activated protein kinase phosphatase 1; MKP-1; MKP1; Protein tyrosine phosphatase CL100; Protein-tyrosine phosphatase CL100; PTPN10; Serine/threonine specific protein phosphatase; VH1;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
P28562 DUS1_HUMAN:

Expressed at high levels in the lung, liver placenta and pancreas. Moderate levels seen in the heart and skeletal muscle. Lower levels found in the brain and kidney.

Description:
Expressed at high levels in the lung, liver placenta and pancreas. Moderate levels seen in the heart and skeletal muscle. Lower levels found in the brain and kidney.
Sequence:
MVMEVGTLDAGGLRALLGERAAQCLLLDCRSFFAFNAGHIAGSVNVRFSTIVRRRAKGAMGLEHIVPNAELRGRLLAGAYHAVVLLDERSAALDGAKRDGTLALAAGALCREARAAQVFFLKGGYEAFSASCPELCSKQSTPMGLSLPLSTSVPDSAESGCSSCSTPLYDQGGPVEILPFLYLGSAYHASRKDMLDALGITALINVSANCPNHFEGHYQYKSIPVEDNHKADISSWFNEAIDFIDSIKNAGGRVFVHCQAGISRSATICLAYLMRTNRVKLDEAFEFVKQRRSIISPNFSFMGQLLQFESQVLAPHCSAEAGSPAMAVLDRGTSTTTVFNFPVSIPVHSTNSALSYLQSPITTSPSC

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Xenopus
88
Zebrafish
88
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P28562 As Substrate

Site PTM Type Enzyme
K57 Acetylation PR:000007102 (EP300)
K57 Ubiquitination
K97 Acetylation
K97 Ubiquitination
K122 Ubiquitination
S131 Phosphorylation
S140 Phosphorylation
T141 Phosphorylation
S146 Phosphorylation
K221 Ubiquitination
K230 Ubiquitination
K280 Ubiquitination
K289 Ubiquitination
S296 Phosphorylation P27361 (MAPK3) , P28482 (MAPK1)
S323 Phosphorylation P27361 (MAPK3) , P28482 (MAPK1)
S334 Phosphorylation P54646 (PRKAA2)
S359 Phosphorylation P28482 (MAPK1) , P27361 (MAPK3)
S364 Phosphorylation P27361 (MAPK3) , P28482 (MAPK1)

Research Backgrounds

Function:

Dual specificity phosphatase that dephosphorylates MAP kinase MAPK1/ERK2 on both 'Thr-183' and 'Tyr-185', regulating its activity during the meiotic cell cycle.

PTMs:

Phosphorylation at Ser-359 and Ser-364 by MAPK1/ERK2 and MAPK3/ERK1 reduces its rate of degradation.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed at high levels in the lung, liver placenta and pancreas. Moderate levels seen in the heart and skeletal muscle. Lower levels found in the brain and kidney.

Family&Domains:

Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.

Research Fields

· Environmental Information Processing > Signal transduction > MAPK signaling pathway.   (View pathway)

· Organismal Systems > Nervous system > Serotonergic synapse.

References

1). All-Trans Retinoic Acid Attenuates Blue Light-Induced Apoptosis of Retinal Photoreceptors by Upregulating MKP-1 Expression. Molecular Neurobiology (PubMed: 33950345) [IF=5.1]

Application: WB    Species: Rat    Sample: retinas

Fig. 5 RA upregulates retinal expression of MKP-1 and inhibits JNK phosphorylation induced by light exposure. a, d Western blots of p-NF- κB/NF-κB, p-JNK/JNK and MKP-1 expression levels in the control and RA-treated groups unexposed to light and at 3 days and 7 days after light exposure. b, c, e Quantitative analysis of western blotting of the phosphorylation levels of NF-κB (b), JNK (c), and MKP-1 (e). Oneway ANOVA analysis followed by Dunnett’s test was used. n = 3 per group, *P < 0.05 and **P < 0.01. LI, light injury; RA, all-trans retinoic acid; JNK, c-Jun N-terminal kinase; NF-κB, nuclear factor-κB; MKP-1, mitogen-activated protein kinase phosphatase-1

2). Rosiglitazone Improves Glucocorticoid Resistance in a Sudden Sensorineural Hearing Loss by Promoting MAP Kinase Phosphatase-1 Expression. Mediators of Inflammation (PubMed: 31217747) [IF=4.6]

Application: IF/ICC    Species: guinea pigs    Sample: spiral ganglion cell

Figure 3: |MKP-1 immunofluorescence staining of cochlear spiral ganglion of pigs in the AP group. MKP-1 stained in the spiral ganglion cell (white arrows). Blue: nuclei stained with DAPI;green: MKP-1 stained in inner hair cell (IHC) and outer hair cell (OHC). Scale bars = 10 μm. The red marker is the stereocilia of hair cells, and the blue marker is the cell nucleus.

Application: WB    Species: guinea pigs    Sample:

Figure 6:| The protein expression of (a) MKP-1 and GR, (b) p38 and p-p38, and (c) NF-κB and p-NF-κB p65 was determined by western blot analysis. Bar graphs show the quantification of the indicated proteins. Data are shown as means ± SD from at least three independent experiments, #p < 0 05 vs. AP group; ##p < 0 01 vs. AP group; ∗p < 0 05 vs. LPS+DEX group; ∗∗p < 0 01 vs. LPS+DEX group.

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Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.