NRF1 Antibody | Affinity Biosciences

WB
Cites

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Western blot analysis of NRF1 expression in HeLa cell lysate.

Western blot analysis of extracts from Hela, using NRF1 Antibody.

Fig.

Product:	NRF1 Antibody
Catalog:	AF5298
Description:	Rabbit polyclonal antibody to NRF1
Application:	WB IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
Mol.Wt.:	55kD, 70kD; 54kD(Calculated).
Uniprot:	Q16656
RRID:	AB_2837783

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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200ul	$350	In stock

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MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(100%)

Clonality:

Polyclonal

Specificity:

NRF1 Antibody detects endogenous levels of total NRF1.

RRID:

AB_2837783
Cite Format: Affinity Biosciences Cat# AF5298, RRID:AB_2837783.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

alpha pal; alpha palindromic binding protein; Alpha palindromic-binding protein; Alpha-pal; locus control region factor 1; NFE2 related factor 1; NRF-1; Nrf1; NRF1_HUMAN; Nuclear respiratory factor 1;

Immunogens

Immunogen:

Uniprot:

Q16656(NRF1_HUMAN) >>Visit HPA database.

Gene(ID):

NRF1(4899)

Expression:

Q16656 NRF1_HUMAN:

Ubiquitously expressed with strongest expression in skeletal muscle.

Description:

Transcription factor that activates the expression of the EIF2S1 (EIF2-alpha) gene. Links the transcriptional modulation of key metabolic genes to cellular growth and development. Implicated in the control of nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication.

Sequence:

Q16656 NRF1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MEEHGVTQTEHMATIEAHAVAQQVQQVHVATYTEHSMLSADEDSPSSPEDTSYDDSDILNSTAADEVTAHLAAAGPVGMAAAAAVATGKKRKRPHVFESNPSIRKRQQTRLLRKLRATLDEYTTRVGQQAIVLCISPSKPNPVFKVFGAAPLENVVRKYKSMILEDLESALAEHAPAPQEVNSELPPLTIDGIPVSVDKMTQAQLRAFIPEMLKYSTGRGKPGWGKESCKPIWWPEDIPWANVRSDVRTEEQKQRVSWTQALRTIVKNCYKQHGREDLLYAFEDQQTQTQATATHSIAHLVPSQTVVQTFSNPDGTVSLIQVGTGATVATLADASELPTTVTVAQVNYSAVADGEVEQNWATLQGGEMTIQTTQASEATQAVASLAEAAVAASQEMQQGATVTMALNSEAAAHAVATLAEATLQGGGQIVLSGETAAAVGALTGVQDANGLVQIPVSMYQTVVTSLAQGNGPVQVAMAPVTTRISDSAVTMDGQAVEVVTLEQ

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Xenopus

100

Zebrafish

100

Rabbit

100

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q16656 As Substrate

Q16656

Site	PTM Type	Source
S39	Phosphorylation	Uniprot
S44	Phosphorylation	Uniprot
S46	Phosphorylation	Uniprot
S47	Phosphorylation	Uniprot
S52	Phosphorylation	Uniprot
T87	Phosphorylation	Uniprot
K92	Ubiquitination	Uniprot
S99	Phosphorylation	Uniprot
T109	Phosphorylation	Uniprot
T118	Phosphorylation	Uniprot
Y122	Phosphorylation	Uniprot
T123	Phosphorylation	Uniprot
S136	Phosphorylation	Uniprot
S138	Phosphorylation	Uniprot
K139	Ubiquitination	Uniprot
K214	Ubiquitination	Uniprot
K221	Acetylation	Uniprot
K226	Acetylation	Uniprot
T259	Phosphorylation	Uniprot
K267	Ubiquitination	Uniprot

Research Backgrounds

Q16656_NRF1_HUMAN

Function:

PTMs:

Phosphorylation enhances DNA binding.

Subcellular Location:

Nucleus.

Tissue Specificity:

Ubiquitously expressed with strongest expression in skeletal muscle.

Subunit Structure:

Homodimer. Binds DNA as a dimer. Interacts with PPRC1.

Family&Domains:

Belongs to the NRF1/Ewg family.

Research Fields

· Environmental Information Processing > Signal transduction > Apelin signaling pathway. (View pathway)

· Human Diseases > Neurodegenerative diseases > Huntington's disease.

References

1). Heat shock protein 22 modulates NRF1/TFAM-dependent mitochondrial biogenesis and DRP1-sparked mitochondrial apoptosis through AMPK-PGC1α signaling pathway to alleviate the early brain injury of subarachnoid hemorrhage in rats. Redox Biology (PubMed: 33472123) [IF=11.4]

Application: WB Species: rat Sample: brain

Fig. 6. Hsp22 regulates PGC1α via AMPK signaling pathway in rats after SAH Beam balance scores, Modified Garcia scores and Brainwater content in various groups. n = 6 per group. (B) Representative photomicrographs of TUNEL staining and quantitative analyses in the indicated groups. n = 4 per group. Scale bar = 100 μm. (C) Typical photomicrographs showing double immunofluorescence staining of PGC1α (green) and NeuN (red) in diverse experimental groups. n = 4 per group. Scale bar = 50 μm. (D) Western blot images and quantitative analyses of p-AMPK/AMPK, PGC1α, Drp1, Nrf1, TFAM, UCP2, Cleaved caspase-3/Caspase-3, Bcl2, Bax, Cytosolic and mitochondrial cytochrome c. n = 6 per group. Bars represent mean ± SD. **P < 0.01, *P < 0.05 vs. Sham group. ##P < 0.01, #P < 0.05 vs. SAH + Vehicle group. &&P < 0.01, &P < 0.05 vs. SAH + hsp22+scramble siRNA. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

2). ApoE deficiency promotes non-alcoholic fatty liver disease in mice via impeding AMPK/mTOR mediated autophagy. LIFE SCIENCES (PubMed: 32304762) [IF=6.1]

Application: WB Species: mouse Sample: liver

Fig. 5. |Mitochondrial function were downregulated in ApoE−/−-HFD mice. (A–D)Representative immunoblots and relative protein levels of PGC1α and NRF1. (E–F)Hepatic expression and relative flourescent intensity of ROS by immunofluorescent staining.

3). Probiotic Yogurt Alleviates High-Fat Diet-Induced Lipid Accumulation and Insulin Resistance in Mice via the Adiponectin Pathway. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY (PubMed: 36695046) [IF=6.1]

4). Perampanel Stimulates Mitochondrial Biogenesis in Neuronal Cells through Activation of the SIRT1/PGC-1α Signaling Pathway. ACS Chemical Neuroscience [IF=5.0]

5). Kcnma1 is involved in mitochondrial homeostasis in diabetes-related skeletal muscle atrophy. The FASEB Journal (PubMed: 36929614) [IF=4.8]

6). Aerobic exercise and metformin on intermuscular adipose tissue (IMAT): insights from multimodal MRI and histological changes in prediabetic rats. Diabetology & metabolic syndrome (PubMed: 37899436) [IF=4.8]

Application: WB Species: Rat Sample: intermuscular adipose tissues (IMATs)

Fig. 6 Characterization of lipid and glucose metabolism in intermuscular adipose tissues (IMATs). Representative western blots (A, C, E, G, I) and quantification of the gene-expression levels of peroxisome proliferators-activated receptor-γ (PPAR-γ) (B), phosphorylated PPAR-γ (p-PPAR-γ; Ser112) (F), nuclear respiratory factor-1 (NRF-1) (J), glucose transporter-4 (GLUT-4) (D), glucose transporter-1 (GLUT-1) (H), and perilipin-5 (Plin-5) (K). The data represent the mean ± the standard error of the mean (n = 5–6/group). CON control, EMA combined therapies + compound-c, EMC combined therapies, EXE moderate exercise, GAPDH glyceraldehyde-3-phosphate dehydrogenase, MET metformin, PRE prediabetes. ap

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NRF1 Antibody - #AF5298