AP2 alpha Antibody - #AF5323
| Product: | AP2 alpha Antibody |
| Catalog: | AF5323 |
| Description: | Rabbit polyclonal antibody to AP2 alpha |
| Application: | WB IHC IF/ICC |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| Mol.Wt.: | 48 kDa; 48kD(Calculated). |
| Uniprot: | P05549 |
| RRID: | AB_2837808 |
Related Downloads
Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# AF5323, RRID:AB_2837808.
Fold/Unfold
Activating enhancer binding protein 2 alpha; Activating enhancer-binding protein 2-alpha; Activator protein 2; AP 2 transcription factor; AP 2alpha; AP-2; AP-2 transcription factor; AP2; AP2 Transcription Factor; AP2-alpha; AP2A_HUMAN; AP2TF; BOFS; FLJ51761; TFAP 2; TFAP 2A; TFAP2; TFAP2A; Transcription factor AP 2 alpha (activating enhancer binding protein 2 alpha); Transcription factor AP-2-alpha; Transcription factor AP2 alpha;
Immunogens
- P05549 AP2A_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MLWKLTDNIKYEDCEDRHDGTSNGTARLPQLGTVGQSPYTSAPPLSHTPNADFQPPYFPPPYQPIYPQSQDPYSHVNDPYSLNPLHAQPQPQHPGWPGQRQSQESGLLHTHRGLPHQLSGLDPRRDYRRHEDLLHGPHALSSGLGDLSIHSLPHAIEEVPHVEDPGINIPDQTVIKKGPVSLSKSNSNAVSAIPINKDNLFGGVVNPNEVFCSVPGRLSLLSSTSKYKVTVAEVQRRLSPPECLNASLLGGVLRRAKSKNGGRSLREKLDKIGLNLPAGRRKAANVTLLTSLVEGEAVHLARDFGYVCETEFPAKAVAEFLNRQHSDPNEQVTRKNMLLATKQICKEFTDLLAQDRSPLGNSRPNPILEPGIQSCLTHFNLISHGFGSPAVCAAVTALQNYLTEALKAMDKMYLSNNPNSHTDNNAKSSDKEEKHRK
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P05549 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K10 | Sumoylation | Uniprot | |
| K10 | Ubiquitination | Uniprot | |
| Y73 | Phosphorylation | Uniprot | |
| S119 | Phosphorylation | Uniprot | |
| K177 | Sumoylation | Uniprot | |
| K177 | Ubiquitination | Uniprot | |
| S181 | Phosphorylation | Uniprot | |
| K184 | Ubiquitination | Uniprot | |
| S185 | Phosphorylation | Uniprot | |
| S187 | Phosphorylation | Uniprot | |
| K197 | Ubiquitination | Uniprot | |
| S219 | Phosphorylation | P54646 (PRKAA2) | Uniprot |
| S223 | Phosphorylation | Uniprot | |
| S225 | Phosphorylation | Uniprot | |
| K226 | Ubiquitination | Uniprot | |
| K228 | Ubiquitination | Uniprot | |
| S239 | Phosphorylation | P17612 (PRKACA) | Uniprot |
| S258 | Phosphorylation | Q15139 (PRKD1) | Uniprot |
| K268 | Ubiquitination | Uniprot | |
| K271 | Ubiquitination | Uniprot | |
| K282 | Ubiquitination | Uniprot | |
| K315 | Ubiquitination | Uniprot | |
| S326 | Phosphorylation | Q15139 (PRKD1) | Uniprot |
| K335 | Ubiquitination | Uniprot | |
| K411 | Ubiquitination | Uniprot | |
| S428 | Phosphorylation | Uniprot | |
| S429 | Phosphorylation | P68400 (CSNK2A1) | Uniprot |
Research Backgrounds
Sequence-specific DNA-binding protein that interacts with inducible viral and cellular enhancer elements to regulate transcription of selected genes. AP-2 factors bind to the consensus sequence 5'-GCCNNNGGC-3' and activate genes involved in a large spectrum of important biological functions including proper eye, face, body wall, limb and neural tube development. They also suppress a number of genes including MCAM/MUC18, C/EBP alpha and MYC. AP-2-alpha is the only AP-2 protein required for early morphogenesis of the lens vesicle. Together with the CITED2 coactivator, stimulates the PITX2 P1 promoter transcription activation. Associates with chromatin to the PITX2 P1 promoter region.
Sumoylated on Lys-10; which inhibits transcriptional activity.
Nucleus.
Binds DNA as a dimer. Can form homodimers or heterodimers with other AP-2 family members. Interacts with WWOX. Interacts with CITED4. Interacts with UBE2I. Interacts with RALBP1 in a complex also containing EPN1 and NUMB during interphase and mitosis. Interacts with KCTD1; this interaction represses transcription activation. Interacts (via C-terminus) with CITED2 (via C-terminus); the interaction stimulates TFAP2A-transcriptional activation. Interacts (via N-terminus) with EP300 (via N-terminus); the interaction requires CITED2. Interacts with KCTD15; this interaction inhibits TFAP2A transcriptional activation.
The PPxY motif mediates interaction with WWOX.
Belongs to the AP-2 family.
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