PIAS4 Antibody - #AF5329

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Product: PIAS4 Antibody
Catalog: AF5329
Description: Rabbit polyclonal antibody to PIAS4
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Sheep, Dog, Chicken
Mol.Wt.: 57 kDa; 57kD(Calculated).
Uniprot: Q8N2W9
RRID: AB_2837814

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Sheep(100%), Dog(100%), Chicken(83%)
Clonality:
Polyclonal
Specificity:
PIAS4 Antibody detects endogenous levels of total PIAS4.
RRID:
AB_2837814
Cite Format: Affinity Biosciences Cat# AF5329, RRID:AB_2837814.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

E3 SUMO protein ligase PIAS4; E3 SUMO-protein ligase PIAS4; FLJ12419; MGC35296; PAIASgamma; PIAS 4; PIAS gamma; PIAS-gamma; Pias4; PIAS4_HUMAN; PIASG; PIASgamma; PIASy; Protein inhibitor of activated STAT 4; Protein inhibitor of activated STAT protein 4; Protein inhibitor of activated STAT protein gamma; Protein inhibitor of activated STAT protein PIASy; Zinc finger MIZ type containing 6; ZMIZ 6; ZMIZ6;

Immunogens

Immunogen:
Uniprot:

Q8N2W9(PIAS4_HUMAN) >>Visit HPA database.

Gene(ID):
PIAS4(51588)
Expression:
Q8N2W9 PIAS4_HUMAN:

Highly expressed in testis and, at lower levels, in spleen, prostate, ovary, colon and peripheral blood leukocytes.

Description:
Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway, the p53 pathway, the Wnt pathway and the steroid hormone signaling pathway.
Sequence:
MAAELVEAKNMVMSFRVSDLQMLLGFVGRSKSGLKHELVTRALQLVQFDCSPELFKKIKELYETRYAKKNSEPAPQPHRPLDPLTMHSTYDRAGAVPRTPLAGPNIDYPVLYGKYLNGLGRLPAKTLKPEVRLVKLPFFNMLDELLKPTELVPQNNEKLQESPCIFALTPRQVELIRNSRELQPGVKAVQVVLRICYSDTSCPQEDQYPPNIAVKVNHSYCSVPGYYPSNKPGVEPKRPCRPINLTHLMYLSSATNRITVTWGNYGKSYSVALYLVRQLTSSELLQRLKTIGVKHPELCKALVKEKLRLDPDSEIATTGVRVSLICPLVKMRLSVPCRAETCAHLQCFDAVFYLQMNEKKPTWMCPVCDKPAPYDQLIIDGLLSKILSECEDADEIEYLVDGSWCPIRAEKERSCSPQGAILVLGPSDANGLLPAPSVNGSGALGSTGGGGPVGSMENGKPGADVVDLTLDSSSSSEDEEEEEEEEEDEDEEGPRPKRRCPFQKGLVPAC

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Dog
100
Chicken
83
Horse
0
Xenopus
0
Zebrafish
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q8N2W9 As Substrate

Site PTM Type Enzyme
A2 Acetylation
K9 Sumoylation
K9 Ubiquitination
S18 Phosphorylation
K31 Ubiquitination
K35 Sumoylation
K35 Ubiquitination
K56 Ubiquitination
K59 Sumoylation
K59 Ubiquitination
K68 Ubiquitination
K69 Sumoylation
K69 Ubiquitination
T99 Phosphorylation
Y108 Phosphorylation
K114 Acetylation
K114 Ubiquitination
K125 Acetylation
K125 Ubiquitination
K128 Sumoylation
K128 Ubiquitination
K135 Sumoylation
K158 Ubiquitination
K187 Ubiquitination
K231 Sumoylation
K231 Ubiquitination
Y274 Phosphorylation
K294 Ubiquitination
K300 Ubiquitination
K306 Sumoylation
K306 Ubiquitination
K330 Ubiquitination
K360 Ubiquitination
K411 Ubiquitination
K504 Ubiquitination

PTMs - Q8N2W9 As Enzyme

Substrate Site Source
P15927 (RPA2) S4 Uniprot
P15927 (RPA2) S8 Uniprot

Research Backgrounds

Function:

Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway, the p53/TP53 pathway, the Wnt pathway and the steroid hormone signaling pathway. Involved in gene silencing. Mediates sumoylation of CEBPA, PARK7, HERC2, MYB, TCF4 and RNF168. In Wnt signaling, represses LEF1 and enhances TCF4 transcriptional activities through promoting their sumoylations. Enhances the sumoylation of MTA1 and may participate in its paralog-selective sumoylation.

PTMs:

Sumoylated. Lys-35 is the main site of sumoylation. Sumoylation is required for TCF4 sumoylation and transcriptional activation. Represses LEF1 transcriptional activity. SUMO1 is the preferred conjugate.

Subcellular Location:

Nucleus>PML body.
Note: Colocalizes with SUMO1 and TCF7L2/TCF4 and LEF1 in a subset of PML (promyelocytic leukemia) nuclear bodies.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Highly expressed in testis and, at lower levels, in spleen, prostate, ovary, colon and peripheral blood leukocytes.

Subunit Structure:

Interacts with AR, AXIN1, GATA2, LEF1, TP53 and STAT1 (IFNG-induced). Binds to AT-rich DNA sequences, known as matrix or scaffold attachment regions (MARs/SARs) (By similarity). Interacts with TICAM1. Interacts with KLF8; the interaction results in SUMO ligation and repression of KLF8 transcriptional activity and of its cell cycle progression into G(1) phase. Interacts with MTA1.

Family&Domains:

The LXXLL motif is a coregulator signature that is essential for transcriptional corepression.

Belongs to the PIAS family.

Research Fields

· Environmental Information Processing > Signal transduction > NF-kappa B signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Jak-STAT signaling pathway.   (View pathway)

· Genetic Information Processing > Folding, sorting and degradation > Ubiquitin mediated proteolysis.   (View pathway)

References

1). Role of SUMOylation of STAT1 in tubular epithelial‑mesenchymal transition induced by high glucose. Molecular Medicine Reports (PubMed: 36601740) [IF=3.4]

Application: WB    Species: Human    Sample: HK-2 cells

Figure 4. High glucose upregulates STAT1 SUMOylation in HK-2 cells. (A) Co-IP suggested that the binding of STAT1 and UBC9 was upregulated under high-glucose conditions. The data were analyzed by Student's t-test. (B) Efficiency of UBC9 knockdown was analyzed by western blotting and was semi-quantified using densitometry. The data were analyzed by one-way ANOVA and Tukey post hoc test. (C and D) Co-IP suggested that the SUMOylation of STAT1 was enhanced under high-glucose conditions. The data were analyzed by one-way ANOVA and Tukey post hoc test. (E) Binding of STAT1 and PIASs was analyzed. Results suggested that PIAS4 mediated the upregulation of STAT1 SUMOylation. The data were analyzed by Student's t-test. (F) Efficiency of PIAS4 knockdown was analyzed by western blotting and was semi-quantified using densitometry. The data were analyzed by one-way ANOVA and LSD post hoc test. The effects of PIAS4 siRNA transfection on STAT1 SUMOylation (G) were analyzed by co-IP and (H) were semi-quantified using densitometry. The results suggested that PIAS4 knockdown inhibited STAT1 SUMOylation. The data were analyzed by one-way ANOVA and Tukey post hoc test. IP:STAT1 means the cell lysate was incubated with STAT1 antibody. Input was used as a positive control to detect the presence of target proteins. n=3. *P

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