MRLC1 Antibody - #AF5423

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Product: MRLC1 Antibody
Catalog: AF5423
Description: Rabbit polyclonal antibody to MRLC1
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 20 kDa; 20kD(Calculated).
Uniprot: P24844
RRID: AB_2837907

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 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
MRLC1 Antibody detects endogenous levels of total MRLC1.
RRID:
AB_2837907
Cite Format: Affinity Biosciences Cat# AF5423, RRID:AB_2837907.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

20 kDa myosin light chain;Human 20kDa myosin light chain (MLC2) mRNA complete cds;LC20;MGC3505;MLC 2;MLC-2C;MLC2;MLY 9;MRLC1;MYL9;MYL9_HUMAN;Myosin light chain 9 regulatory;Myosin light polypeptide 9 regulatory;myosin regulatory light chain 1;Myosin regulatory light chain 2;Myosin regulatory light chain 2 smooth muscle isoform;Myosin regulatory light chain 9;Myosin regulatory light chain MRLC1;Myosin regulatory light polypeptide 9;Myosin RLC;Myosin vascular smooth muscle light chain 2;MYRL2;OTTHUMP00000030857;smooth muscle isoform; ML12B_HUMAN;MLC 2A;MLC-2;MLC-2a;MLC-B;MLC20;MRLC2;MYL12B;MYLC2B;Myosin light chain 12B regulatory;Myosin regulatory light chain 12B;myosin regulatory light chain 2;Myosin regulatory light chain 2-B;myosin regulatory light chain 2-B, smooth muscle isoform;Myosin regulatory light chain 20 kDa;Myosin regulatory light chain MRLC2;myosin, light chain 12B, regulatory;OTTHUMP00000162244;OTTHUMP00000165806;OTTHUMP00000165807;OTTHUMP00000165808;SHUJUN-1;smooth muscle isoform;

Immunogens

Immunogen:
Uniprot:

P24844(MYL9_HUMAN) >>Visit HPA database.

Gene(ID):
MYL9(10398)
Expression:
P24844 MYL9_HUMAN:

Smooth muscle tissues and in some, but not all, nonmuscle cells.

Sequence:
MSSKRAKAKTTKKRPQRATSNVFAMFDQSQIQEFKEAFNMIDQNRDGFIDKEDLHDMLASLGKNPTDEYLEGMMSEAPGPINFTMFLTMFGEKLNGTDPEDVIRNAFACFDEEASGFIHEDHLRELLTTMGDRFTDEEVDEMYREAPIDKKGNFNYVEFTRILKHGAKDKDD

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Chicken
100
Rabbit
100
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P24844 As Substrate

Site PTM Type Enzyme
S2 Phosphorylation P17252 (PRKCA)
S3 Phosphorylation P17252 (PRKCA)
T10 Phosphorylation P17252 (PRKCA)
T11 Phosphorylation
T19 Phosphorylation O43293 (DAPK3) , O14578 (CIT) , Q15746 (MYLK) , Q5VT25 (CDC42BPA) , Q13464 (ROCK1)
S20 Phosphorylation O43293 (DAPK3) , Q5VT25 (CDC42BPA) , O75116 (ROCK2) , Q14012 (CAMK1) , Q15746 (MYLK) , Q13153 (PAK1) , Q13177 (PAK2) , Q9Y5S2 (CDC42BPB) , O14578 (CIT) , Q13464 (ROCK1)
S29 Phosphorylation
K51 Acetylation
K51 Ubiquitination
S60 Phosphorylation
T97 Phosphorylation
S115 Phosphorylation
T128 Phosphorylation
T135 Phosphorylation
Y143 Phosphorylation P00533 (EGFR)
K150 Ubiquitination
K151 Sumoylation
K151 Ubiquitination
Y156 Phosphorylation P00533 (EGFR)
K164 Ubiquitination
K168 Acetylation

Research Backgrounds

Function:

Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Implicated in cytokinesis, receptor capping, and cell locomotion.

PTMs:

Phosphorylation increases the actin-activated myosin ATPase activity and thereby regulates the contractile activity. It is required to generate the driving force in the migration of the cells but not necessary for localization of myosin-2 at the leading edge.

Tissue Specificity:

Smooth muscle tissues and in some, but not all, nonmuscle cells.

Subunit Structure:

Myosin is a hexamer of 2 heavy chains and 4 light chains: interacts with myosin heavy chain MYO19.

Research Fields

· Cellular Processes > Cellular community - eukaryotes > Focal adhesion.   (View pathway)

· Cellular Processes > Cellular community - eukaryotes > Tight junction.   (View pathway)

· Cellular Processes > Cell motility > Regulation of actin cytoskeleton.   (View pathway)

· Environmental Information Processing > Signal transduction > cGMP-PKG signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > cAMP signaling pathway.   (View pathway)

· Organismal Systems > Circulatory system > Vascular smooth muscle contraction.   (View pathway)

· Organismal Systems > Immune system > Leukocyte transendothelial migration.   (View pathway)

· Organismal Systems > Endocrine system > Oxytocin signaling pathway.

References

1). Patchouli alcohol attenuates 5-fluorouracil-induced intestinal mucositis via TLR2/MyD88/NF-kB pathway and regulation of microbiota. BIOMEDICINE & PHARMACOTHERAPY (PubMed: 32004938) [IF=7.5]

Application: WB    Species: rat    Sample: Intestinal

Fig. 4. |Effect of PA on intestinal mocusal barrier proteins. (a–d) Relative mRNA expression of MLCK, ZO-1, occludin and claudin-1 (n = 4); (e–i) Expressions of MLC,p-MLC, ZO-1, occludin and claudin-1proteins (n = 3). Values were represented the mean ± SEM. **P < 0.01, *P < 0.05 versus 5-FU group and ##P < 0.01 versus normal group.
2). A comparative study of the ameliorative effects of hyaluronic acid oligosaccharides and hyaluronic acid on DSS-induced colitis in mice and research on relevant mechanisms. Food & Function [IF=6.1]
3). GLP-1 attenuates Ang II-induced proliferation and migration in rat aorta smooth muscle cells via inhibition of the RhoA/ROCK2 signaling pathway. PHARMAZIE (PubMed: 30522551) [IF=1.6]

Application: WB    Species: rat    Sample: RASMCs

Fig. 5: |Effects of GLP-1, Ex-4, forskolin, Y-27632, and H89 on Ang II-induced RASMC migration. (A and E) Cell migration was measured by the wound-healing assay. (C and G) Protein expression level of p-MLC was evaluated by Western blot. Data are presented as means ± SD (n = 3,* P < 0.05 vs. control, # P < 0.05 vs. Ang II, & P < 0.05 vs. Ang II+GLP-1 or Ang II+Ex-4).

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