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  • Product Name
    Metadherin Antibody
  • Catalog No.
    BF0508
  • RRID
    AB_2833740
  • Source
    Mouse
  • Application
    WB,IHC,ELISA,FCM
  • Reactivity
    Human
  • UniProt
  • Mol.Wt
    64kD;
    64kDa(Calculated).
  • Concentration
    1mg/ml
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Product Information

Alternative Names:Expand▼

3D3; 3D3/LYRIC; AEG 1; AEG-1; AEG1; Astrocyte elevated gene 1; Astrocyte elevated gene-1 protein; LYRIC; LYRIC/3D3; LYRIC_HUMAN; Lysine rich CEACAM1 associated protein; Lysine rich CEACAM1 co isolated protein; Lysine-rich CEACAM1 co-isolated protein; Metadherin; Metastasis adhesion protein; MTDH; Protein LYRIC;

Applications:

ELISA 1:10000, WB 1:500-1:2000, IHC 1:200-1:1000, FCM 1:200-1:400
*The optimal dilutions should be determined by the end user.

Reactivity:

Human

Source:

Mouse

Clonality:

Monoclonal

Clone Number:

AFB1716

Purification:

Affinity-chromatography.

Specificity:

Metadherin antibody detects endogenous levels of total Metadherin.

RRID:

AB_2833740
Please cite this product as: Affinity Biosciences Cat# BF0508, RRID:AB_2833740.

Format:

Liquid

Concentration:

1mg/ml

Storage Condition and Buffer:

Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

Immunogen Information

Immunogen:

Purified recombinant fragment of human Metadherin expressed in E. Coli.

Uniprot:



>>Visit The Human Protein Atlas

Gene ID:

Gene Name:

MTDH

Molecular Weight:

Observed Mol.Wt.: 64kD.
Predicted Mol.Wt.: 64kDa(Calculated)..

Subcellular Location:

Endoplasmic reticulum membrane. Nucleus membrane. Cell junction > tight junction. Nucleus > nucleolus. Cytoplasm > perinuclear region. In epithelial cells, recruited to tight junctions (TJ) during the maturation of the TJ complexes. A nucleolar staining may be due to nuclear targeting of an isoform lacking the transmembrane domain (By similarity). TNF-alpha causes translocation from the cytoplasm to the nucleus.

Tissue Specificity:

Q86UE4 LYRIC_HUMAN:
Widely expressed with highest levels in muscle-dominating organs such as skeletal muscle, heart, tongue and small intestine and in endocrine glands such as thyroid and adrenal gland. Overexpressed in various cancers including breast, brain, prostate, melanoma and glioblastoma multiforme.

Description:

Metadherin (Metastasis adhesion protein), also known as MTDH, LYsine-RIch CEACAM1 co-isolated (LYRIC), is a novel protein that localizes with the tight junction proteins ZO-1 and occludin in polarized epithelial cells. At the tight junction, it acts not as a structural component, but is rather recruited during the maturation of the tight junction complex. Metadherin is overexpressed in breast cancer tissue and breast tumor xenografts, while much lower levels are expressed in normal breast tissue. Metadherin binds to lung vasculature, one of the four common sites of breast cancer metastasis, through a C-terminal segment in the extracellular domain; blocking this lung-homing domain with antibodies or inhibiting metadherin with siRNA has been reported to inhibit breast cancer metastasis.

Sequence:
MAARSWQDELAQQAEEGSARLREMLSVGLGFLRTELGLDLGLEPKRYPGWVILVGTGALGLLLLFLLGYGWAAACAGARKKRRSPPRKREEAAAVPAAAPDDLALLKNLRSEEQKKKNRKKLSEKPKPNGRTVEVAEGEAVRTPQSVTAKQPPEIDKKNEKSKKNKKKSKSDAKAVQNSSRHDGKEVDEGAWETKISHREKRQQRKRDKVLTDSGSLDSTIPGIENTITVTTEQLTTASFPVGSKKNKGDSHLNVQVSNFKSGKGDSTLQVSSGLNENLTVNGGGWNEKSVKLSSQISAGEEKWNSVSPASAGKRKTEPSAWSQDTGDANTNGKDWGRSWSDRSIFSGIGSTAEPVSQSTTSDYQWDVSRNQPYIDDEWSGLNGLSSADPNSDWNAPAEEWGNWVDEERASLLKSQEPIPDDQKVSDDDKEKGEGALPTGKSKKKKKKKKKQGEDNSTAQDTEELEKEIREDLPVNTSKTRPKQEKAFSLKTISTSDPAEVLVKNSQPIKTLPPATSTEPSVILSKSDSDKSSSQVPPILQETDKSKSNTKQNSVPPSQTKSETSWESPKQIKKKKKARRET

Research Background

Function:

Downregulates SLC1A2/EAAT2 promoter activity when expressed ectopically. Activates the nuclear factor kappa-B (NF-kappa-B) transcription factor. Promotes anchorage-independent growth of immortalized melanocytes and astrocytes which is a key component in tumor cell expansion. Promotes lung metastasis and also has an effect on bone and brain metastasis, possibly by enhancing the seeding of tumor cells to the target organ endothelium. Induces chemoresistance.

Subcellular Location:

Endoplasmic reticulum membrane>Single-pass membrane protein. Nucleus membrane>Single-pass membrane protein. Cell junction>Tight junction. Nucleus>Nucleolus. Cytoplasm>Perinuclear region.
Note: In epithelial cells, recruited to tight junctions (TJ) during the maturation of the TJ complexes. A nucleolar staining may be due to nuclear targeting of an isoform lacking the transmembrane domain (By similarity). TNF-alpha causes translocation from the cytoplasm to the nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte

Tissue Specificity:

Widely expressed with highest levels in muscle-dominating organs such as skeletal muscle, heart, tongue and small intestine and in endocrine glands such as thyroid and adrenal gland. Overexpressed in various cancers including breast, brain, prostate, melanoma and glioblastoma multiforme.

Subunit Structure:

Interacts with BCCIP, CREBBP/CBP and RELA/p65.

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Catalog Number :

BF0508-BP
(Blocking peptide available as BF0508-BP)

Price/Size :

$350/1mg.
Tips: For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Function :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

Format and storage :

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.

Precautions :

This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Q86UE4 as Substrate
Site PTM Type Enzyme
S84 Phosphorylation
K88 Ubiquitination
K107 Ubiquitination
S111 Phosphorylation
K121 Ubiquitination
K125 Ubiquitination
K127 Ubiquitination
T143 Phosphorylation
S146 Phosphorylation
T148 Phosphorylation
K150 Ubiquitination
K170 Acetylation
K174 Acetylation
K174 Ubiquitination
S179 Phosphorylation
S180 Phosphorylation
K185 Ubiquitination
K195 Ubiquitination
S197 Phosphorylation
S214 Phosphorylation
S216 Phosphorylation
S219 Phosphorylation
K248 Ubiquitination
S251 Phosphorylation
S258 Phosphorylation
K261 Methylation
K264 Methylation
S267 Phosphorylation
T268 Phosphorylation
S272 Phosphorylation
S273 Phosphorylation
T280 Phosphorylation
K289 Ubiquitination
K292 Ubiquitination
S294 Phosphorylation
S295 Phosphorylation
S298 Phosphorylation
K303 Ubiquitination
S306 Phosphorylation
S308 Phosphorylation
S311 Phosphorylation
K314 Acetylation
K314 Methylation
K314 Ubiquitination
S323 Phosphorylation
K334 Ubiquitination
R338 Methylation
S339 Phosphorylation
S341 Phosphorylation
S344 Phosphorylation
S347 Phosphorylation
S357 Phosphorylation
T361 Phosphorylation
S362 Phosphorylation
Y364 Phosphorylation
S369 Phosphorylation
S411 Phosphorylation
S415 Phosphorylation
S426 Phosphorylation
T439 Phosphorylation
K441 Acetylation
S457 Phosphorylation
T458 Phosphorylation
T462 Phosphorylation
S478 Phosphorylation
K491 Methylation
K491 Ubiquitination
T492 Phosphorylation
S494 Phosphorylation
T495 Phosphorylation
S496 Phosphorylation
S506 Phosphorylation
K510 Ubiquitination
T511 Phosphorylation
S521 Phosphorylation
S527 Phosphorylation
S529 Phosphorylation
S532 Phosphorylation
S534 Phosphorylation
S546 Phosphorylation
S548 Phosphorylation
T550 Phosphorylation
S558 Phosphorylation
T560 Phosphorylation
S562 Phosphorylation
T564 Phosphorylation
S565 Phosphorylation
S568 Phosphorylation
K570 Ubiquitination
T582 Phosphorylation
IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.

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