Product: IKK epsilon Antibody
Catalog: DF6047
Description: Rabbit polyclonal antibody to IKK epsilon
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig
Mol.Wt.: 80kDa; 80kD(Calculated).
Uniprot: Q14164
RRID: AB_2838017

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 100ul $280 In stock
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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(85%)
Clonality:
Polyclonal
Specificity:
IKK epsilon Antibody detects endogenous levels of total IKK epsilon.
RRID:
AB_2838017
Cite Format: Affinity Biosciences Cat# DF6047, RRID:AB_2838017.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

I kappa B kinase epsilon; I-kappa-B kinase epsilon; IkBKE; IKK related kinase epsilon; IKK-E; IKK-epsilon; IKK-i; IKKE; IKKE_HUMAN; IKKepsilon; IKKI; Inducible I kappa B kinase; Inducible I kappa-B kinase; Inducible IkappaB kinase; Inhibitor of kappa light polypeptide gene enhancer in B cells kinase epsilon; Inhibitor of kappa light polypeptide gene enhancer in B cells, kinase of, epsilon; Inhibitor of nuclear factor kappa-B kinase subunit epsilon; KIAA0151; MGC125294; MGC125295; MGC125297;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
Q14164 IKKE_HUMAN:

Highly expressed in spleen followed by thymus, peripheral blood leukocytes, pancreas, placenta. Weakly expressed in lung, kidney, prostate, ovary and colon.

Description:
IKK epsilon is a noncanonical I-kappa-B kinase (IKK) that is essential for regulating antiviral signaling pathways. It has also been identified as a breast cancer oncogene and is amplified and overexpressed in over 30% of breast carcinomas and breast cancer cell lines (1). IKK epsilon phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. It plays a special role in the immune response. IKK epsilon protects cells against DNA damage-induced cell death (2).
Sequence:
MQSTANYLWHTDDLLGQGATASVYKARNKKSGELVAVKVFNTTSYLRPREVQVREFEVLRKLNHQNIVKLFAVEETGGSRQKVLVMEYCSSGSLLSVLESPENAFGLPEDEFLVVLRCVVAGMNHLRENGIVHRDIKPGNIMRLVGEEGQSIYKLTDFGAARELDDDEKFVSVYGTEEYLHPDMYERAVLRKPQQKAFGVTVDLWSIGVTLYHAATGSLPFIPFGGPRRNKEIMYRITTEKPAGAIAGAQRRENGPLEWSYTLPITCQLSLGLQSQLVPILANILEVEQAKCWGFDQFFAETSDILQRVVVHVFSLSQAVLHHIYIHAHNTIAIFQEAVHKQTSVAPRHQEYLFEGHLCVLEPSVSAQHIAHTTASSPLTLFSTAIPKGLAFRDPALDVPKFVPKVDLQADYNTAKGVLGAGYQALRLARALLDGQELMFRGLHWVMEVLQATCRRTLEVARTSLLYLSSSLGTERFSSVAGTPEIQELKAAAELRSRLRTLAEVLSRCSQNITETQESLSSLNRELVKSRDQVHEDRSIQQIQCCLDKMNFIYKQFKKSRMRPGLGYNEEQIHKLDKVNFSHLAKRLLQVFQEECVQKYQASLVTHGKRMRVVHETRNHLRLVGCSVAACNTEAQGVQESLSKLLEELSHQLLQDRAKGAQASPPPIAPYPSPTRKDLLLHMQELCEGMKLLASDLLDNNRIIERLNRVPAPPDV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
85
Horse
69
Sheep
69
Bovine
62
Dog
0
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q14164 As Substrate

Site PTM Type Enzyme
K25 Ubiquitination
K30 Ubiquitination
K61 Ubiquitination
K137 Ubiquitination
Y153 Phosphorylation
K154 Ubiquitination
S172 Phosphorylation Q14164 (IKBKE) , O14920 (IKBKB) , O15111 (CHUK)
Y179 Phosphorylation
K231 Sumoylation
K231 Ubiquitination
K241 Ubiquitination
K401 Ubiquitination
K416 Ubiquitination
T463 Phosphorylation
S464 Phosphorylation
T474 Phosphorylation
S479 Phosphorylation
K490 Ubiquitination
T501 Phosphorylation
S519 Phosphorylation
S522 Phosphorylation
K549 Ubiquitination
K578 Ubiquitination
K609 Ubiquitination
S664 Phosphorylation
Y671 Phosphorylation
S673 Phosphorylation
K677 Ubiquitination

PTMs - Q14164 As Enzyme

Substrate Site Source
O00571 (DDX3X) S102 Uniprot
O14519 (CDK2AP1) S46 Uniprot
O43524 (FOXO3) S644 Uniprot
O43734 (TRAF3IP2) S328 Uniprot
O95786 (DDX58) S855 Uniprot
P03372 (ESR1) S167 Uniprot
P10914 (IRF1) S215 Uniprot
P10914 (IRF1) S219 Uniprot
P10914 (IRF1) S221 Uniprot
P25963 (NFKBIA) S32 Uniprot
P25963 (NFKBIA) S36 Uniprot
P31749 (AKT1) T308 Uniprot
P31749 (AKT1) S473 Uniprot
P42224 (STAT1) S727 Uniprot
P49840 (GSK3A) S21 Uniprot
P98170 (XIAP) S430 Uniprot
Q04206 (RELA) S468 Uniprot
Q04206 (RELA) S536 Uniprot
Q12933 (TRAF2) S11 Uniprot
Q14164 (IKBKE) S172 Uniprot
Q14653 (IRF3) S386 Uniprot
Q14653 (IRF3) S396 Uniprot
Q14653 (IRF3) S398 Uniprot
Q14653 (IRF3) S402 Uniprot
Q5S007 (LRRK2) S910 Uniprot
Q5S007 (LRRK2) S935 Uniprot
Q92844 (TANK) S49 Uniprot
Q92844 (TANK) S100 Uniprot
Q92844 (TANK) S126 Uniprot
Q92844 (TANK) S178 Uniprot
Q92844 (TANK) S208 Uniprot
Q92844 (TANK) S228 Uniprot
Q92844 (TANK) S257 Uniprot
Q92844 (TANK) S406 Uniprot
Q92844 (TANK) S409 Uniprot
Q92985 (IRF7) S471 Uniprot
Q92985 (IRF7) S472 Uniprot
Q92985 (IRF7) S477 Uniprot
Q92985 (IRF7) S479 Uniprot
Q96FA3 (PELI1) S76 Uniprot
Q96FA3 (PELI1) T80 Uniprot
Q96FA3 (PELI1) T288 Uniprot
Q96FA3 (PELI1) S293 Uniprot
Q9NQC7 (CYLD) S418 Uniprot
Q9Y6K9 (IKBKG) T50 Uniprot
Q9Y6K9 (IKBKG) S141 Uniprot
Q9Y6K9 (IKBKG) S148 Uniprot
Q9Y6K9 (IKBKG) S196 Uniprot
Q9Y6K9 (IKBKG) S208 Uniprot
Q9Y6K9 (IKBKG) S247 Uniprot

Research Backgrounds

Function:

Serine/threonine kinase that plays an essential role in regulating inflammatory responses to viral infection, through the activation of the type I IFN, NF-kappa-B and STAT signaling. Also involved in TNFA and inflammatory cytokines, like Interleukin-1, signaling. Following activation of viral RNA sensors, such as RIG-I-like receptors, associates with DDX3X and phosphorylates interferon regulatory factors (IRFs), IRF3 and IRF7, as well as DDX3X. This activity allows subsequent homodimerization and nuclear translocation of the IRF3 leading to transcriptional activation of pro-inflammatory and antiviral genes including IFNB. In order to establish such an antiviral state, IKBKE forms several different complexes whose composition depends on the type of cell and cellular stimuli. Thus, several scaffolding molecules including IPS1/MAVS, TANK, AZI2/NAP1 or TBKBP1/SINTBAD can be recruited to the IKBKE-containing-complexes. Activated by polyubiquitination in response to TNFA and interleukin-1, regulates the NF-kappa-B signaling pathway through, at least, the phosphorylation of CYLD. Phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. In addition, is also required for the induction of a subset of ISGs which displays antiviral activity, may be through the phosphorylation of STAT1 at 'Ser-708'. Phosphorylation of STAT1 at 'Ser-708' seems also to promote the assembly and DNA binding of ISGF3 (STAT1:STAT2:IRF9) complexes compared to GAF (STAT1:STAT1) complexes, in this way regulating the balance between type I and type II IFN responses. Protects cells against DNA damage-induced cell death. Also plays an important role in energy balance regulation by sustaining a state of chronic, low-grade inflammation in obesity, wich leads to a negative impact on insulin sensitivity. Phosphorylates AKT1.

PTMs:

Autophosphorylated and phosphorylated by IKBKB/IKKB. Phosphorylation at Ser-172 is enhanced by the interaction with DDX3X. Phosphorylated at Thr-501 upon IFN activation.

Sumoylation by TOPORS upon DNA damage is required for protection of cells against DNA damage-induced cell death. Desumoylated by SENP1.

'Lys-63'-linked polyubiquitinated at Lys-30 and Lys-401 by TRAF2:BIRC2 and TRAF2:BIRC3 complexes. Ubiquitination is induced by LPS, TNFA and interleukin-1 and required for full kinase activity and KF-kappa-B pathway activation.

Subcellular Location:

Cytoplasm. Nucleus. Nucleus>PML body.
Note: Targeting to PML nuclear bodies upon DNA damage is TOPORS-dependent (PubMed:20188669). Located diffusely throughout the cytoplasm but locates to punctate cytoplasmic bodies when coexpressed with TRIM6 (PubMed:24882218).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Highly expressed in spleen followed by thymus, peripheral blood leukocytes, pancreas, placenta. Weakly expressed in lung, kidney, prostate, ovary and colon.

Subunit Structure:

Homodimer. Interacts with MAVS/IPS1. Interacts (via protein kinase domain) with TTLL12 (via N-terminus); the interaction prevents MAVS binding to IKBKE. Interacts with the adapter proteins AZI2/NAP1, TANK and TBKBP1/SINTBAD. Interacts with SIKE1. Interacts with TICAM1/TRIF, IRF3 and DDX58/RIG-I; interactions are disrupted by the interaction between IKBKE and SIKE1. Interacts with TOPORS; induced by DNA damage. Interacts with CYLD. Interacts (when polyubiquitinated) with IKBKB, IKBKG and MYD88. Interacts with IFIH1. Interacts with DDX3X; the interaction is found to be induced upon virus infection. Interacts with TRIM6 (via SPRY box). Interacts with unanchored K48-linked polyubiquitin chains; this leads to IKBKE activation. Interacts with TBK1.

(Microbial infection) Interacts (via Protein kinase domain) with arenavirus protein N; the interaction inhibits IKBKE kinase function.

(Microbial infection) Interacts with Ebola virus protein VP35; the interaction leads to inhibition of cellular antiviral response by blocking necessary interactions between the IKBKE and MAVS/IPS as well as its substrates IRF3 and IRF7.

(Microbial infection) Interacts with human T-cell leukemia virus 1/HTLV-1 protein HBZ.

Family&Domains:

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. I-kappa-B kinase subfamily.

Research Fields

· Human Diseases > Infectious diseases: Viral > Hepatitis C.

· Human Diseases > Infectious diseases: Viral > Hepatitis B.

· Human Diseases > Infectious diseases: Viral > Measles.

· Human Diseases > Infectious diseases: Viral > Influenza A.

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Human Diseases > Infectious diseases: Viral > Herpes simplex infection.

· Organismal Systems > Immune system > Toll-like receptor signaling pathway.   (View pathway)

· Organismal Systems > Immune system > NOD-like receptor signaling pathway.   (View pathway)

· Organismal Systems > Immune system > RIG-I-like receptor signaling pathway.   (View pathway)

· Organismal Systems > Immune system > Cytosolic DNA-sensing pathway.   (View pathway)

· Organismal Systems > Immune system > IL-17 signaling pathway.   (View pathway)

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