Product: IL6 Antibody
Catalog: DF6087
Source: Rabbit
Application: WB, IHC, IF/ICC, ELISA(peptide)
Reactivity: Human, Mouse, Rat
Prediction: Bovine
Mol.Wt.: 24kD; 24kD(Calculated).
Uniprot: P05231
RRID: AB_2838055

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:200, IF/ICC 1:200, ELISA(peptide) 1:20000-1:40000
*The optimal dilutions should be determined by the end user.
Reactivity:
Human,Mouse,Rat
Prediction:
Bovine(100%)
Clonality:
Polyclonal
Specificity:
IL6 Antibody detects endogenous levels of total IL6.
RRID:
AB_2838055
Cite Format: Affinity Biosciences Cat# DF6087, RRID:AB_2838055.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Interleukin BSF 2; B cell differentiation factor; B cell stimulatory factor 2; B-cell stimulatory factor 2; BSF 2; BSF-2; BSF2; CDF; CTL differentiation factor; Hepatocyte stimulatory factor; HGF; HSF; Hybridoma growth factor; Hybridoma growth factor Interferon beta-2; IFN-beta-2; IFNB2; IL 6; IL-6; IL6; IL6_HUMAN; Interferon beta 2; Interferon beta-2; Interleukin 6; Interleukin 6 (interferon beta 2); Interleukin BSF 2; Interleukin-6;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Description:
Interleukin-6 (IL-6) is a multifunctional cytokine with a wide variety of biological functions. IL-6 is implicated in the final differentiation of B-cells into immunoglobulin-secreting cells (1), myeloma and plasmacytoma growth (2), nerve cell differentiation, and activation of hepatocytes and mitogen-stimulated helper T cells (3). Upon activation, IL-6 induces at least three major signaling pathways: JAK/STAT, PI-3 kinase and MAPK (4,5).
Sequence:
MNSFSTSAFGPVAFSLGLLLVLPAAFPAPVPPGEDSKDVAAPHRQPLTSSERIDKQIRYILDGISALRKETCNKSNMCESSKEALAENNLNLPKMAEKDGCFQSGFNEETCLVKIITGLLEFEVYLEYLQNRFESSEEQARAVQMSTKVLIQFLQKKAKNLDAITTPDPTTNASLLTKLQAQNQWLQDMTTHLILRSFKEFLQSSLRALRQM

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Bovine
100
Dog
78
Pig
71
Horse
71
Sheep
71
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P05231 As Substrate

Site PTM Type Enzyme
N73 N-Glycosylation
S81 Phosphorylation
K94 Ubiquitination
S135 Phosphorylation
S136 Phosphorylation
T165 Phosphorylation

Research Backgrounds

Function:

Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. Required for the generation of T(H)17 cells. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation.

PTMs:

N- and O-glycosylated.

Subcellular Location:

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Interacts with IL6R (via the N-terminal ectodomain); this interaction may be affected by IL6R-binding with SORL1, hence decreasing IL6 cis signaling. Interacts with SORL1 (via the N-terminal ectodomain); this interaction leads to IL6 internalization and lysosomal degradation. May form a trimeric complex with the soluble SORL1 ectodomain and soluble IL6R receptor; this interaction might stabilize circulating IL6, hence promoting IL6 trans signaling.

Family&Domains:

Belongs to the IL-6 superfamily.

Research Fields

· Cellular Processes > Cell growth and death > Cellular senescence.   (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > Cytokine-cytokine receptor interaction.   (View pathway)

· Environmental Information Processing > Signal transduction > HIF-1 signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > FoxO signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Jak-STAT signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > TNF signaling pathway.   (View pathway)

· Human Diseases > Drug resistance: Antineoplastic > EGFR tyrosine kinase inhibitor resistance.

· Human Diseases > Drug resistance: Antineoplastic > Antifolate resistance.

· Human Diseases > Endocrine and metabolic diseases > Insulin resistance.

· Human Diseases > Endocrine and metabolic diseases > Non-alcoholic fatty liver disease (NAFLD).

· Human Diseases > Neurodegenerative diseases > Prion diseases.

· Human Diseases > Infectious diseases: Bacterial > Salmonella infection.

· Human Diseases > Infectious diseases: Bacterial > Pertussis.

· Human Diseases > Infectious diseases: Bacterial > Legionellosis.

· Human Diseases > Infectious diseases: Parasitic > Chagas disease (American trypanosomiasis).

· Human Diseases > Infectious diseases: Parasitic > African trypanosomiasis.

· Human Diseases > Infectious diseases: Parasitic > Malaria.

· Human Diseases > Infectious diseases: Parasitic > Amoebiasis.

· Human Diseases > Infectious diseases: Bacterial > Tuberculosis.

· Human Diseases > Infectious diseases: Viral > Hepatitis B.

· Human Diseases > Infectious diseases: Viral > Measles.

· Human Diseases > Infectious diseases: Viral > Influenza A.

· Human Diseases > Infectious diseases: Viral > HTLV-I infection.

· Human Diseases > Infectious diseases: Viral > Herpes simplex infection.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Overview > Transcriptional misregulation in cancer.

· Human Diseases > Immune diseases > Inflammatory bowel disease (IBD).

· Human Diseases > Immune diseases > Rheumatoid arthritis.

· Human Diseases > Immune diseases > Graft-versus-host disease.

· Human Diseases > Cardiovascular diseases > Hypertrophic cardiomyopathy (HCM).

· Organismal Systems > Immune system > Toll-like receptor signaling pathway.   (View pathway)

· Organismal Systems > Immune system > NOD-like receptor signaling pathway.   (View pathway)

· Organismal Systems > Immune system > Cytosolic DNA-sensing pathway.   (View pathway)

· Organismal Systems > Immune system > Hematopoietic cell lineage.   (View pathway)

· Organismal Systems > Immune system > IL-17 signaling pathway.   (View pathway)

· Organismal Systems > Immune system > Th17 cell differentiation.   (View pathway)

· Organismal Systems > Immune system > Intestinal immune network for IgA production.   (View pathway)

References

1). Li X et al. Upregulation of BCL-2 by acridone derivative through gene promoter i-motif for alleviating liver damage of NAFLD/NASH. Nucleic Acids Res 2020 Sep 4;48(15):8255-8268. (PubMed: 32710621) [IF=11.501]

Application: WB    Species: mouse    Sample: liver

Figure 7. Effect of A22 on ameliorating apoptosis, ER stress, inflammation, metabolic syndrome, and fibrogenesis in HF diet-fed mice. (A) Effect of A22 on BCL-2 gene transcription. (B) Effect of A22 on BAX gene transcription. (C) Effect of A22 on expressions of apoptosis-related proteins in liver. The extracted proteins from the liver were immunoblotted with specific antibodies, and quantified based on the loading control of ACTIN. (D) Effect of A22 on ER stress. The UPR proteins (IRE-1, PERK, elF-2 and CHOP) were analyzed by using western Blot. (E) Effect of A22 on expressions of inflammatory factors. (F) Effect of A22 on expressions of fibrogenic proteins.

2). Ou W et al. Hypoxic acclimation improves cardiac redox homeostasis and protects heart against ischemia-reperfusion injury through upregulation of O-GlcNAcylation. Redox Biol 2021 Jul;43:101994. (PubMed: 33964586) [IF=9.986]

Application: WB    Species: rat    Sample: H9c2 cells

Fig. 3. | HA-induced inflammation stimulated protein O-GlcNAcylation in hearts. A, Immunoblot analysis of IL-1β and IL-6 in the Control and HA hearts (n = 9 per group). B, Immunoblot analysis of IL-1β and IL-6 in the H9c2 cells (IL-1β: n = 7 per group; IL-6: n = 8 per group).

3). Rao Y et al. A novel HSF1 activator ameliorates non‐alcoholic steatohepatitis by stimulating mitochondrial adaptive oxidation. Br J Pharmacol 2022 Apr;179(7):1411-1432. (PubMed: 34783017) [IF=7.730]

4). Zhao YZ et al. Ulcerative colitis-specific delivery of keratinocyte growth factor by neutrophils-simulated liposomes facilitates the morphologic and functional recovery of the damaged colon through alleviating the inflammation. J Control Release 2019 Feb 23 (PubMed: 30807805) [IF=7.727]

Application: IHC    Species: mouse    Sample: macrophages

Fig. 10.| Alleviation of inflammation and normalization of blood vessel inside inflamed colon after treatment with KGF-Neus: A)Immunohistochemistry staining of CD68 (marker of macrophages, red arrow indicated the positive), IL-6 (interleukin-6) and Immunofluorescence staining of VEGF (vascular endothelial growth factor),CD31 (neovascular platelet endothelial cell adhesion molecule-1)

5). Chen LJ et al. Single xenotransplant of rat brown adipose tissue prolonged the ovarian lifespan of aging mice by improving follicle survival. Aging Cell 2019 Aug 6:e13024 (PubMed: 31389140) [IF=7.238]

Application: WB    Species: mouse    Sample: BAT(brown adipose tissue)

FIGURE 6|RTM significantly improved the levels of important adipokines of aging mice. (a) The levels of IL6 and adiponectin in BAT were significantly higher in RTM, MTM, and young groups than in the aging group. (b) Quantification of (a). (c) The levels of IL6 and adiponectin in plasma were significantly higher in RTM, MTM, and young groups than in the aging group. (d) Quantification of (c). *p < 0.05, **p < 0.01, ***p < 0.001 are considered significantly different

6). Zhang Y et al. pH-responsive hierarchical H2S-releasing nano-disinfectant with deep-penetrating and anti-inflammatory properties for synergistically enhanced eradication of bacterial biofilms and wound infection. J Nanobiotechnology 2022 Jan 29;20(1):55. (PubMed: 35093073) [IF=6.518]

7). He XF et al. NLRP3-dependent microglial training impaired the clearance of amyloid-beta and aggravated the cognitive decline in Alzheimer’s disease. Cell Death Dis 2020 Oct 13;11(10):849. (PubMed: 33051464) [IF=6.304]

Application: WB    Species: mice    Sample: cortex and hippocampus

Fig. 3 Histological and western blotting analysis of microglial activation, pro-inflammatory cytokines, and histone deacetylase (Hdac)1/2 levels. A Immunofluorescence staining of Iba1 in the cortex and hippocampus. B Comparisons of the numbers of Iba1-positive microglia in the cortex and hippocampus (×40 objective). C Chemiluminescence images of IL-6, Hdac1, and GAPDH. D Comparisons of the IL-6/GAPDH, Hdac1/ GAPDH ratios. E Chemiluminescence images of TNF-α, Hdac2, and GAPDH. F Comparisons of the TNF-α/GAPDH, Hdac2/GAPDH ratios. Each dataset is expressed as mean ± SD. *P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001; ****P ≤ 0.0001. n = 6 mice.

8). Shao W et al. In situ mucoadhesive hydrogel capturing tripeptide KPV: the anti-inflammatory, antibacterial and repairing effect on chemotherapy-induced oral mucositis. Biomater Sci 2021 Dec 21;10(1):227-242. (PubMed: 34846053) [IF=6.183]

9). Liu W et al. Micheliolide Ameliorates Diabetic Kidney Disease by Inhibiting Mtdh-mediated Renal Inflammation in Type 2 Diabetic db/db Mice. Pharmacol Res 2019 Oct 24:104506 (PubMed: 31669149) [IF=5.893]

10). Qiu LL et al. Dysregulation of BDNF/TrkB signaling mediated by NMDAR/Ca2+/calpain might contribute to postoperative cognitive dysfunction in aging mice. J Neuroinflammation 2020 Jan 16;17(1):23 (PubMed: 31948437) [IF=5.793]

Application: WB    Species: Mice    Sample: hippocampus

Fig. 3 Increased hippocampal levels of IL-1β, IL-6, and NMDAR subunits after anesthesia and surgery were attenuated by MEM treatment on day 1 post-surgery. a Representative Western blots of IL-1β in the hippocampus. GAPDH was included as a loading control. b Quantitative analysis of IL-1β levels. c Representative Western blots of IL-6 in the hippocampus. GAPDH was included as loading control. d Quantitative analysis of IL-6 levels. e Representative Western blots of TNF-α in the hippocampus. GAPDH was included as loading control. f Quantitative analysis of TNF-α levels. g Representative Western blots of GluN2A. GAPDH was included as loading control. h Quantitative analysis of GluN2A levels. i Representative Western blots of GluN2B. GAPDH was included as loading control. j Quantitative analysis of GluN2B levels. Data are presented as the mean ± SEM (n = 6). *p < 0.05 compared to the con + veh group (**p < 0.01, ***p < 0.001), # p < 0.05 compared to the sur + veh group (##p < 0.01, ###p < 0.001)

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