GLP1 Antibody | Affinity Biosciences

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Product:	GLP1 Antibody
Catalog:	DF6255
Description:	Rabbit polyclonal antibody to GLP1
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Dog
Mol.Wt.:	21kDa; 21kD(Calculated).
Uniprot:	P01275
RRID:	AB_2838221

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%)

Clonality:

Polyclonal

Specificity:

GLP1 Antibody detects endogenous levels of total GLP1.

RRID:

AB_2838221
Cite Format: Affinity Biosciences Cat# DF6255, RRID:AB_2838221.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

GCG; GLP-1; GLP-1(7-36); GLP-1(7-37); GLP-2; GLUC_HUMAN; Glucagon-like peptide 2; GRPP; Incretin hormone; OXM; OXY;

Immunogens

Immunogen:

Uniprot:

P01275(GLUC_HUMAN) >>Visit HPA database.

Gene(ID):

GCG(2641)

Expression:

P01275 GLUC_HUMAN:

Glucagon is secreted in the A cells of the islets of Langerhans. GLP-1, GLP-2, oxyntomodulin and glicentin are secreted from enteroendocrine cells throughout the gastrointestinal tract. GLP-1 and GLP-2 are also secreted in selected neurons in the brain.

Description:

Glucose homeostasis is regulated by a variety of hormones including glucagon. Glucagon is synthesized as the precursor molecule proglucagon and is proteolytically processed to yield the mature peptide in α cells of the pancreatic islets. Glucagon causes the release of glucose from glycogen and stimulates gluconeogenesis in the liver (1,2).

Sequence:

P01275 GLUC_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MKSIYFVAGLFVMLVQGSWQRSLQDTEEKSRSFSASQADPLSDPDQMNEDKRHSQGTFTSDYSKYLDSRRAQDFVQWLMNTKRNRNNIAKRHDEFERHAEGTFTSDVSSYLEGQAAKEFIAWLVKGRGRRDFPEEVAIVEELGRRHADGSFSDEMNTILDNLAARDFINWLIQTKITDRK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Xenopus

Zebrafish

Chicken

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P01275 As Substrate

P01275

Site	PTM Type	Source
T26	Phosphorylation	Uniprot
S32	Phosphorylation	Uniprot
S34	Phosphorylation	Uniprot
S36	Phosphorylation	Uniprot
S42	Phosphorylation	Uniprot
S54	Phosphorylation	Uniprot
Y62	Phosphorylation	Uniprot
S68	Phosphorylation	Uniprot
S150	Phosphorylation	Uniprot
S152	Phosphorylation	Uniprot

Research Backgrounds

P01275_GLUC_HUMAN

Function:

Glucagon plays a key role in glucose metabolism and homeostasis. Regulates blood glucose by increasing gluconeogenesis and decreasing glycolysis. A counterregulatory hormone of insulin, raises plasma glucose levels in response to insulin-induced hypoglycemia. Plays an important role in initiating and maintaining hyperglycemic conditions in diabetes.

GLP-1 is a potent stimulator of glucose-dependent insulin release. Plays important roles on gastric motility and the suppression of plasma glucagon levels. May be involved in the suppression of satiety and stimulation of glucose disposal in peripheral tissues, independent of the actions of insulin. Has growth-promoting activities on intestinal epithelium. May also regulate the hypothalamic pituitary axis (HPA) via effects on LH, TSH, CRH, oxytocin, and vasopressin secretion. Increases islet mass through stimulation of islet neogenesis and pancreatic beta cell proliferation. Inhibits beta cell apoptosis.

GLP-2 stimulates intestinal growth and up-regulates villus height in the small intestine, concomitant with increased crypt cell proliferation and decreased enterocyte apoptosis. The gastrointestinal tract, from the stomach to the colon is the principal target for GLP-2 action. Plays a key role in nutrient homeostasis, enhancing nutrient assimilation through enhanced gastrointestinal function, as well as increasing nutrient disposal. Stimulates intestinal glucose transport and decreases mucosal permeability.

Oxyntomodulin significantly reduces food intake. Inhibits gastric emptying in humans. Suppression of gastric emptying may lead to increased gastric distension, which may contribute to satiety by causing a sensation of fullness.

Glicentin may modulate gastric acid secretion and the gastro-pyloro-duodenal activity. May play an important role in intestinal mucosal growth in the early period of life.

PTMs:

Proglucagon is post-translationally processed in a tissue-specific manner in pancreatic A cells and intestinal L cells. In pancreatic A cells, the major bioactive hormone is glucagon cleaved by PCSK2/PC2. In the intestinal L cells PCSK1/PC1 liberates GLP-1, GLP-2, glicentin and oxyntomodulin. GLP-1 is further N-terminally truncated by post-translational processing in the intestinal L cells resulting in GLP-1(7-37) GLP-1-(7-36)amide. The C-terminal amidation is neither important for the metabolism of GLP-1 nor for its effects on the endocrine pancreas.

Subcellular Location:

Secreted.

Tissue Specificity:

Family&Domains:

Belongs to the glucagon family.

References

1). The active GLP-1 analogue liraglutide alleviates H9N2 influenza virus-induced acute lung injury in mice. MICROBIAL PATHOGENESIS (PubMed: 33285220) [IF=3.8]

Application: WB Species: Mice Sample: lung tissues

Fig. 2. GCG mRNA expression and GCG precursor protein are increased, but total GLP-1 and active GLP-1 levels are decreased in mice during infection. (A) GCG mRNA expression in the lungs of mock and H9N2 groups (n = 5). (B) Representative western blotting bands of the GCG precursor protein in the lungs of mock and H9N2 groups. (C) Data of GCG precursor protein expression in the lungs of mock and H9N2 groups (n = 4). (D) Representative immunohistochemistry images of GCG precursor protein expressed in the lungs of mock and H9N2 groups on 7 d.p.i. The results were similar in five mice of per group. GCG precursor protein positive cells was noted by red arrows. (E) Total GLP-1 in the lungs of mock and H9N2 groups (n = 5). (F) Total GLP-1 in the serum of mock and H9N2 groups (n = 5). (G) Active GLP- 1 in the lungs of mock and H9N2 groups (n = 5). (H) Active GLP-1 in the serum of mock and H9N2 groups (n = 5). (I) Active GLP-1/total GLP-1 in the lungs of mock and H9N2 groups on 5 and 7d.p.i. (n = 5). (J) Active GLP-1/total GLP-1 in the serum of mock and H9N2 groups on 5 and 7d.p.i. (n = 5). Data are presented as mean ± SD, analyzed using Sidak’s or Tukey’s multiple comparisons tests. * = P < 0.05, ** = P < 0.01, *** = P < 0.001, **** = P < 0.0001, NS = non-significant. GCG, glucagon. GLP-1, glucagon like peptide-1. d.p.i., days post infection.

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GLP1 Antibody - #DF6255