Price Size
$280 100ul
$350 200ul

Same day delivery

Contact distributor
  • Product Name
    Prealbumin Antibody
  • Catalog No.
    DF6256
  • RRID
    AB_2838222
  • Source
    Rabbit
  • Application
    WB,IHC,ELISA
  • Reactivity
    Human, Mouse, Rat
  • Prediction
    Pig(100%), Zebrafish(83%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(86%), Dog(100%), Chicken(86%)
  • UniProt
  • Mol.Wt
    16kD;
    16kDa(Calculated).
  • Concentration
    1mg/ml
  • Browse similar products>>

Related Products

Product Information

Alternative Names:Expand▼

Amyloid polyneuropathy; Amyloidosis I; ATTR; Carpal tunnel syndrome 1; CTS; CTS1; Dysprealbuminemic euthyroidal hyperthyroxinemia; Dystransthyretinemic hyperthyroxinemia; Epididymis luminal protein 111; HEL111; HsT2651; PALB; Prealbumin amyloidosis type I; Prealbumin; Prealbumin Thyroxine-binding; Senile systemic amyloidosis; TBPA; Thyroxine binding prealbumin; Transthyretin; TTHY_HUMAN; TTR; TTR protein;

Applications:

WB 1:500-1:2000, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000
*The optimal dilutions should be determined by the end user.

Reactivity:

Human, Mouse, Rat

Predicted Reactivity:

Pig(100%), Zebrafish(83%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(86%), Dog(100%), Chicken(86%)

Source:

Rabbit

Clonality:

Polyclonal

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Specificity:

Prealbumin Antibody detects endogenous levels of total Prealbumin.

RRID:

AB_2838222
Please cite this product as: Affinity Biosciences Cat# DF6256, RRID:AB_2838222.

Format:

Liquid

Concentration:

1mg/ml

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

Immunogen Information

Immunogen:

A synthesized peptide derived from human Prealbumin, corresponding to a region within C-terminal amino acids.

Uniprot:



>>Visit The Human Protein Atlas

Gene ID:

Gene Name:

TTR

Molecular Weight:

Observed Mol.Wt.: 16kD.
Predicted Mol.Wt.: 16kDa(Calculated)..

Subcellular Location:

Secreted. Cytoplasm.

Tissue Specificity:

P02766 TTHY_HUMAN:
Detected in serum and cerebrospinal fluid (at protein level). Highly expressed in choroid plexus epithelial cells. Detected in retina pigment epithelium and liver.

Description:

This gene encodes transthyretin, one of the three prealbumins including alpha-1-antitrypsin, transthyretin and orosomucoid. Transthyretin is a carrier protein; it transports thyroid hormones in the plasma and cerebrospinal fluid, and also transports retinol (vitamin A) in the plasma. The protein consists of a tetramer of identical subunits. More than 80 different mutations in this gene have been reported; most mutations are related to amyloid deposition, affecting predominantly peripheral nerve and/or the heart, and a small portion of the gene mutations is non-amyloidogenic. The diseases caused by mutations include amyloidotic polyneuropathy, euthyroid hyperthyroxinaemia, amyloidotic vitreous opacities, cardiomyopathy, oculoleptomeningeal amyloidosis, meningocerebrovascular amyloidosis, carpal tunnel syndrome, etc. [provided by RefSeq, Jan 2009]

Sequence:
MASHRLLLLCLAGLVFVSEAGPTGTGESKCPLMVKVLDAVRGSPAINVAVHVFRKAADDTWEPFASGKTSESGELHGLTTEEEFVEGIYKVEIDTKSYWKALGISPFHEHAEVVFTANDSGPRRYTIAALLSPYSYSTTAVVTNPKE

Research Background

Function:

Thyroid hormone-binding protein. Probably transports thyroxine from the bloodstream to the brain.

Post-translational Modifications:

Not glycosylated under normal conditions. Following unfolding, caused for example by variant AMYL-TTR 'Gly-38', the cryptic Asn-118 site is exposed and glycosylated by STT3B-containing OST complex, leading to its degradation by the ER-associated degradation (ERAD) pathway.

Subcellular Location:

Secreted. Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte

Tissue Specificity:

Detected in serum and cerebrospinal fluid (at protein level). Highly expressed in choroid plexus epithelial cells. Detected in retina pigment epithelium and liver.

Subunit Structure:

Homotetramer. Dimer of dimers. In the homotetramer, subunits assemble around a central channel that can accommodate two ligand molecules. Interacts with RBP4.

Similarity:

Each monomer has two 4-stranded beta sheets and the shape of a prolate ellipsoid. Antiparallel beta-sheet interactions link monomers into dimers. A short loop from each monomer forms the main dimer-dimer interaction. These two pairs of loops separate the opposed, convex beta-sheets of the dimers to form an internal channel.

Belongs to the transthyretin family.

Research Fields

Research Fields:

· Organismal Systems > Endocrine system > Thyroid hormone synthesis.

Reference Citations:

1). Zhang P et al. Tetraiodothyroacetic acid and transthyretin silencing inhibit pro-metastatic effect of L-thyroxin in anoikis-resistant prostate cancer cells through regulation of MAPK/ERK pathway. Exp Cell Res 2016 Oct 1;347(2):350-9 (PubMed: 27569004) [IF=3.383]

Application: WB    Species:human;    Sample:HepG2

(B) Western blotting indicated that expression of TTR gradually increased along with enhanced metastatic potential 23 in different prostate cancer cell lines (HepG2 cells were used as a positive control). (C) Western blotting indicated that together with TTR, expressions of XIAP, MMP-2 and VEGF were remarkably higher in anoikis-resistant PC-3 cells in contrast to that of parental cells


No comment
Total 0 records, divided into1 pages. First Prev Next Last

Submit Review

Support JPG, GIF, PNG format only
captcha
Catalog Number :

DF6256-BP
(Blocking peptide available as DF6256-BP)

Price/Size :

$350/1mg.
Tips: For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Function :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

Format and storage :

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.

Precautions :

This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Pig
100%
Sheep
100%
Dog
100%
Horse
100%
Bovine
100%
Rabbit
86%
Chicken
86%
Zebrafish
83%
Xenopus
0%
High similarity Medium similarity Low similarity No similarity
P02766 as Substrate
Site PTM Type Enzyme
C10 S-Nitrosylation
T23 Phosphorylation
T25 Phosphorylation
S72 Phosphorylation
N118 N-Glycosylation
Y125 Phosphorylation
S137 Phosphorylation
T138 Phosphorylation
IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.

To Top