CRH Antibody - #DF6258

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Product: CRH Antibody
Catalog: DF6258
Description: Rabbit polyclonal antibody to CRH
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Sheep, Dog
Mol.Wt.: 21kDa; 21kD(Calculated).
Uniprot: P06850
RRID: AB_2838224

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(92%), Sheep(92%), Dog(92%)
Clonality:
Polyclonal
Specificity:
CRH Antibody detects endogenous levels of total CRH.
RRID:
AB_2838224
Cite Format: Affinity Biosciences Cat# DF6258, RRID:AB_2838224.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Corticoliberin; Corticotropin releasing factor; Corticotropin releasing hormone; CRF; Crh; CRH1;

Immunogens

Immunogen:
Uniprot:

P06850(CRF_HUMAN) >>Visit HPA database.

Gene(ID):
CRH(1392)
Expression:
P06850 CRF_HUMAN:

Produced by the hypothalamus and placenta.

Description:
Corticotropin-releasing hormone is secreted by the paraventricular nucleus (PVN) of the hypothalamus in response to stress. Marked reduction in this protein has been observed in association with Alzheimer disease and autosomal recessive hypothalamic corticotropin deficiency has multiple and potentially fatal metabolic consequences including hypoglycemia and hepatitis. In addition to production in the hypothalamus, this protein is also synthesized in peripheral tissues, such as T lymphocytes and is highly expressed in the placenta. In the placenta it is a marker that determines the length of gestation and the timing of parturition and delivery. A rapid increase in circulating levels of the hormone occurs at the onset of parturition, suggesting that, in addition to its metabolic functions, this protein may act as a trigger for parturition. [provided by RefSeq, Apr 2010]
Sequence:
MRLPLLVSAGVLLVALLPCPPCRALLSRGPVPGARQAPQHPQPLDFFQPPPQSEQPQQPQARPVLLRMGEEYFLRLGNLNKSPAAPLSPASSLLAGGSGSRPSPEQATANFFRVLLQQLLLPRRSLDSPAALAERGARNALGGHQEAPERERRSEEPPISLDLTFHLLREVLEMARAEQLAQQAHSNRKLMEIIGK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
92
Sheep
92
Dog
92
Horse
0
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P06850 As Substrate

Site PTM Type Enzyme
S125 Phosphorylation

Research Backgrounds

Function:

Hormone regulating the release of corticotropin from pituitary gland (By similarity). Induces NLRP6 in intestinal epithelial cells, hence may influence gut microbiota profile (By similarity).

Subcellular Location:

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Produced by the hypothalamus and placenta.

Subunit Structure:

Interacts (via C-terminus) with CRFR1 (via N-terminal extracellular domain).

Family&Domains:

Belongs to the sauvagine/corticotropin-releasing factor/urotensin I family.

Research Fields

· Human Diseases > Substance dependence > Alcoholism.

· Organismal Systems > Nervous system > Long-term depression.

References

1). Corticotropin-releasing factor induces inflammatory cytokines via the NLRP6-inflammatory cytokine axis in a murine model of irritable bowel syndrome. Journal of Digestive Diseases (PubMed: 30663229) [IF=3.5]

Application: WB    Species: mouse    Sample: colonic

FIGURE 2 |Corticotropin-releasing factor (CRF) inhibition of NOD-like receptor pyrin domain-containing protein 6 (NLRP6) expression and Clodistrium butyricum (C. butyricum) restoration of CRF expression in (A-D) the control, irritable bowel syndrome (IBS) model and C. butyricumtreated groups (n = 6 per group) or (E,F) Caco-2 cells. (A) Western blot analysis and (B) gray scale values measured by Quantity One software of CRF expression in colonic tissues.

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