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$280 100ul
$350 200ul

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  • Product Name
    RRM1 Antibody
  • Catalog No.
    DF6286
  • RRID
    AB_2838252
  • Source
    Rabbit
  • Application
    WB,IHC,IF/ICC,ELISA(peptide)
  • Reactivity
    Human, Mouse, Rat
  • Prediction
    Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
  • UniProt
  • Mol.Wt
    90kD;
    90kDa(Calculated).
  • Concentration
    1mg/ml
  • Browse similar products>>

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Product Information

Alternative Names:Expand▼

R1; Ribonucleoside diphosphate reductase large subunit; Ribonucleoside diphosphate reductase M1 chain; Ribonucleoside diphosphate reductase subunit M1; Ribonucleoside reductase, large subunit; Ribonucleoside-diphosphate reductase large subunit; Ribonucleoside-diphosphate reductase subunit M1; Ribonucleotide reductase large chain; Ribonucleotide reductase large subunit; Ribonucleotide reductase M1; Ribonucleotide reductase M1 polypeptide; Ribonucleotide reductase R1 subunit; Ribonucleotide reductase, M1 subunit; RIR 1; RIR1; RIR1_HUMAN; RR 1; RR1; RRM 1; RRM1;

Applications:

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000
*The optimal dilutions should be determined by the end user.

Reactivity:

Human, Mouse, Rat

Predicted Reactivity:

Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus

Source:

Rabbit

Clonality:

Polyclonal

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Specificity:

RRM1 Antibody detects endogenous levels of total RRM1.

RRID:

AB_2838252
Please cite this product as: Affinity Biosciences Cat# DF6286, RRID:AB_2838252.

Format:

Liquid

Concentration:

1mg/ml

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

Immunogen Information in 3D

Immunogen:

A synthesized peptide derived from human RRM1, corresponding to a region within the internal amino acids.

Uniprot:



>>Visit The Human Protein Atlas

Gene ID:

Gene Name:

RRM1

Molecular Weight:

Observed Mol.Wt.: 90kD.
Predicted Mol.Wt.: 90kDa(Calculated)..

Subcellular Location:

Cytoplasm.

Description:

Ribonucleotide reductase catalyzes the rate-limiting step in the synthesis of deoxynucleotide triphosphates (dNTPs). The regulatory M1 subunit (RRM1) is present throughout the cell division cycle, but downregulated in quiescent cells (1). Research studies have demonstrated that RRM1 is involved in carcinogenesis and tumor progression, and its expression is correlated with resistance to chemotherapy in non-small cell lung cancer (2-4).

Sequence:
MHVIKRDGRQERVMFDKITSRIQKLCYGLNMDFVDPAQITMKVIQGLYSGVTTVELDTLAAETAATLTTKHPDYAILAARIAVSNLHKETKKVFSDVMEDLYNYINPHNGKHSPMVAKSTLDIVLANKDRLNSAIIYDRDFSYNYFGFKTLERSYLLKINGKVAERPQHMLMRVSVGIHKEDIDAAIETYNLLSERWFTHASPTLFNAGTNRPQLSSCFLLSMKDDSIEGIYDTLKQCALISKSAGGIGVAVSCIRATGSYIAGTNGNSNGLVPMLRVYNNTARYVDQGGNKRPGAFAIYLEPWHLDIFEFLDLKKNTGKEEQRARDLFFALWIPDLFMKRVETNQDWSLMCPNECPGLDEVWGEEFEKLYASYEKQGRVRKVVKAQQLWYAIIESQTETGTPYMLYKDSCNRKSNQQNLGTIKCSNLCTEIVEYTSKDEVAVCNLASLALNMYVTSEHTYDFKKLAEVTKVVVRNLNKIIDINYYPVPEACLSNKRHRPIGIGVQGLADAFILMRYPFESAEAQLLNKQIFETIYYGALEASCDLAKEQGPYETYEGSPVSKGILQYDMWNVTPTDLWDWKVLKEKIAKYGIRNSLLIAPMPTASTAQILGNNESIEPYTSNIYTRRVLSGEFQIVNPHLLKDLTERGLWHEEMKNQIIACNGSIQSIPEIPDDLKQLYKTVWEISQKTVLKMAAERGAFIDQSQSLNIHIAEPNYGKLTSMHFYGWKQGLKTGMYYLRTRPAANPIQFTLNKEKLKDKEKVSKEEEEKERNTAAMVCSLENRDECLMCGS

Research Background

Function:

Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides.

Subcellular Location:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte

Subunit Structure:

Heterodimer of a large and a small subunit. Heterodimer with small subunit RRM2 or RRM2B. The heterodimer with RRM2 has higher catalytic activity than the heterodimer with RRM2B. Interacts with AHCYL1 which inhibits its activity.

Similarity:

Belongs to the ribonucleoside diphosphate reductase large chain family.

Research Fields

Research Fields:

· Metabolism > Nucleotide metabolism > Purine metabolism.
· Metabolism > Nucleotide metabolism > Pyrimidine metabolism.
· Metabolism > Metabolism of other amino acids > Glutathione metabolism.
· Metabolism > Xenobiotics biodegradation and metabolism > Drug metabolism - other enzymes.
· Metabolism > Global and overview maps > Metabolic pathways.

Reference Citations:

1). Xia G et al. Gambogic acid sensitizes gemcitabine efficacy in pancreatic cancer by reducing the expression of ribonucleotide reductase subunit-M2 (RRM2). J Exp Clin Cancer Res 2017 Aug 10;36(1):107 (PubMed: 28797284)

Application: WB    Species:human;    Sample:Not available


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Catalog Number :

DF6286-BP
(Blocking peptide available as DF6286-BP)

Price/Size :

$350/1mg.
Tips: For modified antibodies, we provide modified peptides(0.5mg) and non-modified peptides(0.5mg).

Function :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

Format and storage :

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.

Precautions :

This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Pig
100%
Horse
100%
Bovine
100%
Sheep
100%
Dog
100%
Rabbit
100%
Xenopus
90%
Zebrafish
80%
Chicken
0%
High similarity Medium similarity Low similarity No similarity
P23921 as Substrate
Site PTM Type Enzyme
K5 Ubiquitination
K17 Acetylation
K17 Ubiquitination
K24 Ubiquitination
K70 Ubiquitination
Y74 Phosphorylation
R80 Methylation
K88 Ubiquitination
K92 Ubiquitination
Y102 Phosphorylation
Y104 Phosphorylation
K111 Ubiquitination
K118 Ubiquitination
S119 Phosphorylation
K128 Ubiquitination
S133 Phosphorylation
S142 Phosphorylation
K149 Ubiquitination
K158 Ubiquitination
K162 Ubiquitination
K180 Ubiquitination
Y232 Phosphorylation
K236 Ubiquitination
K243 Ubiquitination
S253 Phosphorylation
K320 Methylation
K320 Ubiquitination
K369 Ubiquitination
K376 Acetylation
K376 Ubiquitination
K414 Ubiquitination
S415 Phosphorylation
T422 Phosphorylation
K424 Ubiquitination
K464 Ubiquitination
K465 Ubiquitination
K471 Ubiquitination
K479 Ubiquitination
Y485 Phosphorylation
Y486 Phosphorylation
K496 Acetylation
K496 Ubiquitination
K548 Ubiquitination
Y553 Phosphorylation
T555 Phosphorylation
Y556 Phosphorylation
S559 Phosphorylation
K563 Ubiquitination
K582 Ubiquitination
K587 Ubiquitination
K590 Ubiquitination
T604 Phosphorylation
S631 Phosphorylation
K643 Ubiquitination
K656 Ubiquitination
K677 Ubiquitination
K681 Ubiquitination
K689 Ubiquitination
K693 Ubiquitination
Y726 Phosphorylation
K729 Ubiquitination
K733 Ubiquitination
Y738 Phosphorylation
T751 Phosphorylation
K754 Ubiquitination
K762 Ubiquitination
K765 Ubiquitination
K770 Ubiquitination
T774 Phosphorylation
S780 Phosphorylation
S792 Phosphorylation
IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.

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