Product: FCGR1A Antibody
Catalog: DF6330
Description: Rabbit polyclonal antibody to FCGR1A
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Bovine, Sheep, Dog
Mol.Wt.: 43kDa; 43kD(Calculated).
Uniprot: P12314
RRID: AB_2838294

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 100ul $280 In stock
 200ul $350 In stock

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Bovine(82%), Sheep(83%), Dog(92%)
Clonality:
Polyclonal
Specificity:
FCGR1A Antibody detects endogenous levels of total FCGR1A.
RRID:
AB_2838294
Cite Format: Affinity Biosciences Cat# DF6330, RRID:AB_2838294.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CD64; CD64A; Fc fragment of IgG high affinity Ia receptor (CD64); Fc fragment of IgG, high affinity Ia, receptor for; Fc gamma receptor; Fc gamma receptor I A1; Fc gamma RI; Fc gamma RIA; Fc-gamma RI; Fc-gamma RIA; FCG1; FcgammaRIa; FCGR1; FCGR1_HUMAN; FCGR1A; FcRI; FLJ18345; High affinity immunoglobulin gamma Fc receptor I; IGFR1; IgG Fc receptor I; Immunoglobulin G Fc receptor I; OTTHUMP00000013913;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
P12314 FCGR1_HUMAN:

Monocyte/macrophage specific.

Description:
This gene encodes a protein that plays an important role in the immune response. This protein is a high-affinity Fc-gamma receptor. The gene is one of three related gene family members located on chromosome 1. [provided by RefSeq, Jul 2008]
Sequence:
MWFLTTLLLWVPVDGQVDTTKAVITLQPPWVSVFQEETVTLHCEVLHLPGSSSTQWFLNGTATQTSTPSYRITSASVNDSGEYRCQRGLSGRSDPIQLEIHRGWLLLQVSSRVFTEGEPLALRCHAWKDKLVYNVLYYRNGKAFKFFHWNSNLTILKTNISHNGTYHCSGMGKHRYTSAGISVTVKELFPAPVLNASVTSPLLEGNLVTLSCETKLLLQRPGLQLYFSFYMGSKTLRGRNTSSEYQILTARREDSGLYWCEAATEDGNVLKRSPELELQVLGLQLPTPVWFHVLFYLAVGIMFLVNTVLWVTIRKELKRKKKWDLEISLDSGHEKKVISSLQEDRHLEEELKCQEQKEEQLQEGVHRKEPQGAT

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Dog
92
Sheep
83
Bovine
82
Pig
75
Horse
0
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P12314 As Substrate

Site PTM Type Enzyme
N59 N-Glycosylation
T73 Phosphorylation
S74 Phosphorylation
S76 Phosphorylation
N78 N-Glycosylation
Y133 Phosphorylation
Y137 Phosphorylation
Y138 Phosphorylation
N152 N-Glycosylation
N159 N-Glycosylation
N163 N-Glycosylation
N195 N-Glycosylation
S328 Phosphorylation
S340 Phosphorylation

Research Backgrounds

Function:

High affinity receptor for the Fc region of immunoglobulins gamma. Functions in both innate and adaptive immune responses.

PTMs:

Phosphorylated on serine residues.

Subcellular Location:

Cell membrane>Single-pass type I membrane protein.
Note: Stabilized at the cell membrane through interaction with FCER1G.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Monocyte/macrophage specific.

Subunit Structure:

Interacts with FCERG1; forms a functional signaling complex. Interacts with FLNA; prevents FCGR1A degradation. Interacts with EPB41L2, LAT and PPL. Interacts with HCK and LYN.

Family&Domains:

Belongs to the immunoglobulin superfamily. FCGR1 family.

Research Fields

· Cellular Processes > Transport and catabolism > Phagosome.   (View pathway)

· Human Diseases > Infectious diseases: Parasitic > Leishmaniasis.

· Human Diseases > Infectious diseases: Bacterial > Staphylococcus aureus infection.

· Human Diseases > Infectious diseases: Bacterial > Tuberculosis.

· Human Diseases > Cancers: Overview > Transcriptional misregulation in cancer.

· Human Diseases > Cancers: Specific types > Acute myeloid leukemia.   (View pathway)

· Human Diseases > Immune diseases > Systemic lupus erythematosus.

· Organismal Systems > Development > Osteoclast differentiation.   (View pathway)

· Organismal Systems > Immune system > Hematopoietic cell lineage.   (View pathway)

· Organismal Systems > Immune system > Fc gamma R-mediated phagocytosis.   (View pathway)

References

1). FcγRIIb expression on B cells is associated with treatment efficacy for acute rejection after kidney transplantation. MOLECULAR IMMUNOLOGY (PubMed: 28360016) [IF=3.6]

Application: WB    Species: rat    Sample:

Fig. 3. Effects of IVIG, PNGase F IVIG, and CD28 monoclonal antibody on Fc receptor expressions in peripheral blood and spleen in rat models of acute rejection after kidney transplantation. Expressions of FcRIA, FcRIIIA, and FcRIIB in the serum (A) and spleen tissues (B) of rats were determined by western blot. -a

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