Glycophorin C/GPC Antibody - #DF6364

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Product: Glycophorin C/GPC Antibody
Catalog: DF6364
Description: Rabbit polyclonal antibody to Glycophorin C/GPC
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 14kDa; 14kD(Calculated).
Uniprot: P04921
RRID: AB_2838328

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(92%), Bovine(92%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(92%)
Clonality:
Polyclonal
Specificity:
Glycophorin C/GPC Antibody detects endogenous levels of total Glycophorin C/GPC.
RRID:
AB_2838328
Cite Format: Affinity Biosciences Cat# DF6364, RRID:AB_2838328.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CD236; CD236R; GE; GLPC; GLPC_HUMAN; Glycoconnectin; Glycophorin C (Gerbich blood group); Glycophorin D; Glycophorin-C; Glycophorin-D; Glycoprotein beta; GPC; GPD; Gypc; GYPD; MGC117309; MGC126191; MGC126192; PAS 2; PAS 2'; PAS-2''; Sialoglycoprotein D;

Immunogens

Immunogen:
Uniprot:

P04921(GLPC_HUMAN) >>Visit HPA database.

Gene(ID):
GYPC(2995)
Expression:
P04921 GLPC_HUMAN:

Glycophorin-C is expressed in erythrocytes. Glycophorin-D and IsoGPC are ubiquitously expressed.

Description:
Glycophorin C (GYPC) is an integral membrane glycoprotein. It is a minor species carried by human erythrocytes, but plays an important role in regulating the mechanical stability of red cells. A number of glycophorin C mutations have been described. The Gerbich and Yus phenotypes are due to deletion of exon 3 and 2, respectively. The Webb and Duch antigens, also known as glycophorin D, result from single point mutations of the glycophorin C gene. The glycophorin C protein has very little homology with glycophorins A and B. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Feb 2012]
Sequence:
MWSTRSPNSTAWPLSLEPDPGMASASTTMHTTTIAEPDPGMSGWPDGRMETSTPTIMDIVVIAGVIAAVAIVLVSLLFVMLRYMYRHKGTYHTNEAKGTEFAESADAALQGDPALQDAGDSSRKEYFI

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Dog
100
Chicken
100
Rabbit
100
Bovine
92
Xenopus
92
Zebrafish
92
Horse
0
Sheep
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P04921 As Substrate

Site PTM Type Enzyme
S3 O-Glycosylation
T4 O-Glycosylation
S6 O-Glycosylation
N8 N-Glycosylation
S9 O-Glycosylation
T10 O-Glycosylation
S15 O-Glycosylation
S24 O-Glycosylation
S26 O-Glycosylation
T27 O-Glycosylation
T28 O-Glycosylation
T31 O-Glycosylation
T32 O-Glycosylation
T33 O-Glycosylation
S42 O-Glycosylation
K97 Ubiquitination
S104 Phosphorylation
S122 Phosphorylation
Y126 Phosphorylation

Research Backgrounds

Function:

This protein is a minor sialoglycoprotein in human erythrocyte membranes. The blood group Gerbich antigens and receptors for Plasmodium falciparum merozoites are most likely located within the extracellular domain. Glycophorin-C plays an important role in regulating the stability of red cells.

PTMs:

O-glycosylated with core 1 or possibly core 8 glycans.

Subcellular Location:

Cell membrane>Single-pass type III membrane protein.
Note: Linked to the membrane via band 4.1.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Glycophorin-C is expressed in erythrocytes. Glycophorin-D and IsoGPC are ubiquitously expressed.

Family&Domains:

Belongs to the glycophorin-C family.

Research Fields

· Human Diseases > Infectious diseases: Parasitic > Malaria.

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