Product: BDNF Antibody
Catalog: DF6387
Source: Rabbit
Application: WB, IHC, IF/ICC, ELISA(peptide)
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 15kD(mature), 28kD(precursor), 45kD(unprocessed); 28kD(Calculated).
Uniprot: P23560
RRID: AB_2838350

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000
*The optimal dilutions should be determined by the end user.
Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
BDNF Antibody detects endogenous levels of total BDNF.
RRID:
AB_2838350
Cite Format: Affinity Biosciences Cat# DF6387, RRID:AB_2838350.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Abrineurin; ANON2; BDNF; BDNF_HUMAN; Brain Derived Neurotrophic Factor; Brain-derived neurotrophic factor; BULN2; MGC34632; Neurotrophin;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
P23560 BDNF_HUMAN:

Detected in blood plasma and in saliva (at protein level) (PubMed:11152678, PubMed:19467646). Brain. Highly expressed in hippocampus, amygdala, cerebral cortex and cerebellum. Also expressed in heart, lung, skeletal muscle, testis, prostate and placenta.

Description:
Neurotrophins function to regulate naturally occurring cell death of neurons during development. The prototype neurotrophin is nerve growth factor (NGF), originally discovered in the 1950s as a soluble peptide promoting the survival of, and neurite outgrowth from, sympathetic ganglia. Three additional structurally homologous neurotrophic factors have been identified. These include brain-derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3) and neurotrophin-4 (NT-4) (also designated NT-5). These various neurotrophins stimulate the in vitro survival of distinct, but partially overlapping, populations of neurons. The cell surface receptors through which neurotrophins mediate their activity have been identified. For instance, the Trk A receptor is the preferential receptor for NGF, but also binds NT-3 and NT-4. The Trk B receptor binds both BDNF and NT-4 equally well, and binds NT-3 to a lesser extent, while the Trk C receptor only binds NT-3.
Sequence:
MTILFLTMVISYFGCMKAAPMKEANIRGQGGLAYPGVRTHGTLESVNGPKAGSRGLTSLADTFEHVIEELLDEDQKVRPNEENNKDADLYTSRVMLSSQVPLEPPLLFLLEEYKNYLDAANMSMRVRRHSDPARRGELSVCDSISEWVTAADKKTAVDMSGGTVTVLEKVPVSKGQLKQYFYETKCNPMGYTKEGCRGIDKRHWNSQCRTTQSYVRALTMDSKKRIGWRFIRIDTSCVCTLTIKRGR

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Zebrafish
100
Chicken
100
Rabbit
100
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P23560 As Substrate

Site PTM Type Enzyme
K50 Ubiquitination
T62 Phosphorylation O96017 (CHEK2)
K85 Acetylation
K85 Ubiquitination
T155 Phosphorylation
K174 Ubiquitination

Research Backgrounds

Function:

Important signaling molecule that activates signaling cascades downstream of NTRK2. During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability.

Important signaling molecule that activates signaling cascades downstream of NTRK2. Activates signaling cascades via the heterodimeric receptor formed by NGFR and SORCS2. Signaling via NGFR and SORCS2 plays a role in synaptic plasticity and long-term depression (LTD). Binding to NGFR and SORCS2 promotes neuronal apoptosis. Promotes neuronal growth cone collapse (By similarity).

PTMs:

N-glycosylated and glycosulfated, contrary to mature BDNF.

Mature BDNF is produced by proteolytic removal of the propeptide, catalyzed by a FURIN family member. In addition, the precursor form is proteolytically cleaved within the propeptide, but this is not an obligatory intermediate for the production of mature BDNF. Can be converted into mature BDNF by plasmin (PLG).

Subcellular Location:

Secreted.

Secreted.
Note: A proportion of BDNF is secreted as immature precursor (proBDNF).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Detected in blood plasma and in saliva (at protein level). Brain. Highly expressed in hippocampus, amygdala, cerebral cortex and cerebellum. Also expressed in heart, lung, skeletal muscle, testis, prostate and placenta.

Subunit Structure:

Monomers and homodimers. Binds to NTRK2/TRKB. Can form heterodimers with other neurotrophin family members, such as NTF3 and NTF4 (in vitro), but the physiological relevance of this is not clear. BDNF precursor form: interacts with the heterodimer formed by NGFR and SORCS2.

Family&Domains:

Belongs to the NGF-beta family.

Research Fields

· Environmental Information Processing > Signal transduction > MAPK signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Ras signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > cAMP signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Human Diseases > Neurodegenerative diseases > Huntington's disease.

· Human Diseases > Substance dependence > Cocaine addiction.

· Human Diseases > Substance dependence > Alcoholism.

· Organismal Systems > Nervous system > Neurotrophin signaling pathway.   (View pathway)

References

1). Wang X et al. MANF Promotes Diabetic Corneal Epithelial Wound Healing and Nerve Regeneration by Attenuating Hyperglycemia-Induced Endoplasmic Reticulum Stress. Diabetes 2020 Apr 20 (PubMed: 32312869) [IF=7.720]

Application: WB    Species: mice    Sample: corneal epithelial cells

Figure 3—Exogenous MANF promoted corneal nerve regeneration and BDNF and NGF expression. Normal and diabetic corneas pretreated through subconjunctival injection with PBS (as the control) or rhMANF 24 h before, 0 h, and 24 h after removal of the corneal epithelium. At 48 h, corneal epithelial cells were collected for Western blotting. At 5 days postwounding, corneal whole-mount staining was performed to observe nerve regeneration. Corneal sensation was tested before epithelium debridement until 7 days postwounding. To observe nerve regeneration and corneal sensation, another injection was performed at 48 h postwounding. A: Staining for b-tubulin III was performed, and images of the entire cornea were captured as shown in the top panels, with central cornea images shown in the bottom panels. B: Nerve densities of entire corneas (A, top panels) were calculated from the areas staining positive for b-tubulin III using Image J software (n 5 3). C: Corneal sensation is represented with a line chart (n 5 5). The time immediately before epithelium debridement was defined as 0 h. D: Western blot bands observed with or without rhMANF. Two samples/lane represent each condition, three corneas as one sample. E: Western blot analysis of BDNF expression (n 5 4 samples). F: Western blot analysis of NGF expression (n 5 4 samples). *P , 0.05, **P , 0.01, ***P , 0.001 (one-way ANOVA). DM, wounded mice with diabetes mellitus; DM 1 MANF, wounded mice with diabetes mellitus treated with rhMANF; NL, wounded normoglycemic mice treated with PBS; NL 1 MANF, wounded normoglycemic mice treated with rhMANF; n.s., not significant.

2). Bian N et al. Low-Intensity Pulsed Ultrasound Stimulation Inhibits Cortical Spreading Depression. Cereb Cortex 2021 Apr 15;bhab055. (PubMed: 33860305) [IF=5.043]

Application: WB    Species: rat    Sample: brain

Figure 5. |Modulation effect of PUS on BDNF expression in CSD rat. (A) Expression of BDNF in different groups (B) Quantitative analysis of expression of BDNF in different groups (mean ± SD, ∗P < 0.05, ∗∗∗P < 0.01, one-way ANOVA).

3). Hu M et al. Newly generated neurons at 2 months post-status epilepticus are functionally integrated into neuronal circuitry in mouse hippocampus. Exp Neurol 2015 Nov;273:273-87 (PubMed: 26384773) [IF=4.691]

4). Wu J et al. Zhile Capsule Exerts Antidepressant-Like Effects through Upregulation of the BDNF Signaling Pathway and Neuroprotection. Int J Mol Sci 2019 Jan 8;20(1) (PubMed: 30625977) [IF=4.556]

Application: WB    Species: rat    Sample: hippocampus

Figure 6.| ZL activated cAMP-CREB-BDNF signaling. (A–C) Protein expressions of BDNF, p-CREB and CREB in the hippocampus at the end of experiment determined by western blotting.Quantified protein level was normalized to that of GAPDH using densitometry (n = 3)

5). Gao HE et al. Effects of Lifelong Exercise on Age-Related Body Composition, Oxidative Stress, Inflammatory Cytokines, and Skeletal Muscle Proteome in Rats. Mech Ageing Dev 2020 May 15;111262. (PubMed: 32422206) [IF=4.304]

Application: WB    Species: rat    Sample: gastrocnemius muscles

Fig. 6. |Expression of AKT/FOXO1 signaling pathway (A); mitochondrial function markers(B); BDNF signaling-related proteins (C); and representative confocal microscopy images of BDNF staining (green, magnification: ×40,scale Bar 50 μm) (D: a, 8 M–SED; b, 26 M–SED;c, 18 M–MICT; d, 8 M–MICT); and serum BDNF levels (E); and correlation analysis (F).

Application: IF/ICC    Species: rat    Sample: gastrocnemius muscles

Fig. 6. |Expression of AKT/FOXO1 signaling pathway (A); mitochondrial function markers(B); BDNF signaling-related proteins (C); and representative confocal microscopy images of BDNF staining (green, magnification: ×40,scale Bar 50 μm) (D: a, 8 M–SED; b, 26 M–SED;c, 18 M–MICT; d, 8 M–MICT); and serum BDNF levels (E); and correlation analysis (F).

6). Zhang LL et al. Antidepressant-like Effect of Citrus sinensis (L.) Osbeck Essential Oil and Its Main Component Limonene on Mice. J Agric Food Chem 2019 Apr 2 (PubMed: 30905156) [IF=4.192]

7). Gao R et al. Recurrent non-severe hypoglycemia aggravates cognitive decline in diabetes and induces mitochondrial dysfunction in cultured astrocytes. Mol Cell Endocrinol 2021 Apr 15;526:111192. (PubMed: 33545179) [IF=3.871]

8). Xu D et al. XQ-1H alleviates cerebral ischemia in mice through inhibition of apoptosis and promotion of neurogenesis in a Wnt/β-catenin signaling dependent way. Life Sci 2019 Sep 6;235:116844 (PubMed: 31499069) [IF=3.647]

9). Zhang H et al. Effects of Compound 511 on BDNF-TrkB Signaling in the Mice Ventral Tegmental Area in Morphine-Induced Conditioned Place Preference. Cell Mol Neurobiol 2020 Apr 22 (PubMed: 32323150) [IF=3.606]

10). Ge QD et al. MiR-132, miR-204 and BDNF-TrkB signaling pathway may be involved in spatial learning and memory impairment of the offspring rats caused by fluorine and aluminum exposure during the embryonic stage and into adulthood. Environ Toxicol Pharmacol 2018 Oct;63:60-68 (PubMed: 30172012) [IF=3.292]

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