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$350 200ul

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  • Product Name
    TET1 Antibody
  • Catalog No.
    DF6428
  • Source
    Rabbit
  • Application
    WB,IP,ELISA
  • Reactivity:
    Human, Mouse, Rat
  • Prediction:
    Pig(100%), Bovine(100%), Dog(100%)
  • UniProt
  • Mol.Wt.
    235kDa
  • Concentration
    1mg/ml
  • Browse similar products>>

Product Information

Alternative Names:Expand▼

bA119F7.1; CXXC 6; CXXC finger 6; CXXC type zinc finger protein 6; CXXC zinc finger 6; CXXC-type zinc finger protein 6; CXXC6; KIAA1676; LCX; Leukemia associated protein with a CXXC domain; Leukemia-associated protein with a CXXC domain; Methylcytosine dioxygenase TET1; Ten eleven translocation 1; Ten eleven translocation 1 gene protein; Ten eleven translocation 1 gene protein homolog; Ten-eleven translocation 1 gene protein; Tet 1; Tet methylcytosine dioxygenase 1; Tet oncogene 1; TET1; TET1_HUMAN;

Applications:

WB 1:500-1:2000, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000

Reactivity:

Human, Mouse, Rat

Predicted Reactivity:

Pig(100%), Bovine(100%), Dog(100%)

Source:

Rabbit

Clonality:

Polyclonal

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Specificity:

TET1 Antibody detects endogenous levels of total TET1.

Format:

Liquid

Concentration:

1mg/ml

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

Immunogen Information

Immunogen:

A synthesized peptide derived from human TET1 , corresponding to a region within the internal amino acids.

Uniprot:



>>Visit The Human Protein Atlas

Gene id:

Molecular Weight:

Observed Mol.Wt.: 235kDa.
Predicted Mol.Wt.: 236kDa.

Subcellular Location:

Nucleus.

Tissue Specificity:

Expressed in fetal heart, lung and brain, and in adult skeletal muscle, thymus and ovary. Not detected in adult heart, lung or brain. Up-regulated in glioblastoma cells (at protein level) (PubMed:25284789).

Description:

TET1 (tet oncogene 1), also known as LCX or CXXC6, is a 2,136 amino acid protein that localizes to the nucleus and contains one CXXC-type zinc finger. Expressed in adult ovary, thymus and skeletal muscle and also present in fetal lung, heart and brain, TET1 is thought to play a role in the development of fetal organs and may also be involvement in the pathoegenesis and metastasis of acute myeloid leukemia (AML). The gene encoding TET1 maps to human chromosome 10, which houses over 1,200 genes and comprises nearly 4.5% of the human genome. Defects in some of the genes that map to chromosome 10 are associated with Charcot-Marie Tooth disease, Jackson-Weiss syndrome, Usher syndrome, nonsyndromatic deafness, Wolman’s syndrome, Cowden syndrome, multiple endocrine neoplasia type 2 and porphyria.

Sequence:
        10         20         30         40         50
MSRSRHARPS RLVRKEDVNK KKKNSQLRKT TKGANKNVAS VKTLSPGKLK
60 70 80 90 100
QLIQERDVKK KTEPKPPVPV RSLLTRAGAA RMNLDRTEVL FQNPESLTCN
110 120 130 140 150
GFTMALRSTS LSRRLSQPPL VVAKSKKVPL SKGLEKQHDC DYKILPALGV
160 170 180 190 200
KHSENDSVPM QDTQVLPDIE TLIGVQNPSL LKGKSQETTQ FWSQRVEDSK
210 220 230 240 250
INIPTHSGPA AEILPGPLEG TRCGEGLFSE ETLNDTSGSP KMFAQDTVCA
260 270 280 290 300
PFPQRATPKV TSQGNPSIQL EELGSRVESL KLSDSYLDPI KSEHDCYPTS
310 320 330 340 350
SLNKVIPDLN LRNCLALGGS TSPTSVIKFL LAGSKQATLG AKPDHQEAFE
360 370 380 390 400
ATANQQEVSD TTSFLGQAFG AIPHQWELPG ADPVHGEALG ETPDLPEIPG
410 420 430 440 450
AIPVQGEVFG TILDQQETLG MSGSVVPDLP VFLPVPPNPI ATFNAPSKWP
460 470 480 490 500
EPQSTVSYGL AVQGAIQILP LGSGHTPQSS SNSEKNSLPP VMAISNVENE
510 520 530 540 550
KQVHISFLPA NTQGFPLAPE RGLFHASLGI AQLSQAGPSK SDRGSSQVSV
560 570 580 590 600
TSTVHVVNTT VVTMPVPMVS TSSSSYTTLL PTLEKKKRKR CGVCEPCQQK
610 620 630 640 650
TNCGECTYCK NRKNSHQICK KRKCEELKKK PSVVVPLEVI KENKRPQREK
660 670 680 690 700
KPKVLKADFD NKPVNGPKSE SMDYSRCGHG EEQKLELNPH TVENVTKNED
710 720 730 740 750
SMTGIEVEKW TQNKKSQLTD HVKGDFSANV PEAEKSKNSE VDKKRTKSPK
760 770 780 790 800
LFVQTVRNGI KHVHCLPAET NVSFKKFNIE EFGKTLENNS YKFLKDTANH
810 820 830 840 850
KNAMSSVATD MSCDHLKGRS NVLVFQQPGF NCSSIPHSSH SIINHHASIH
860 870 880 890 900
NEGDQPKTPE NIPSKEPKDG SPVQPSLLSL MKDRRLTLEQ VVAIEALTQL
910 920 930 940 950
SEAPSENSSP SKSEKDEESE QRTASLLNSC KAILYTVRKD LQDPNLQGEP
960 970 980 990 1000
PKLNHCPSLE KQSSCNTVVF NGQTTTLSNS HINSATNQAS TKSHEYSKVT
1010 1020 1030 1040 1050
NSLSLFIPKS NSSKIDTNKS IAQGIITLDN CSNDLHQLPP RNNEVEYCNQ
1060 1070 1080 1090 1100
LLDSSKKLDS DDLSCQDATH TQIEEDVATQ LTQLASIIKI NYIKPEDKKV
1110 1120 1130 1140 1150
ESTPTSLVTC NVQQKYNQEK GTIQQKPPSS VHNNHGSSLT KQKNPTQKKT
1160 1170 1180 1190 1200
KSTPSRDRRK KKPTVVSYQE NDRQKWEKLS YMYGTICDIW IASKFQNFGQ
1210 1220 1230 1240 1250
FCPHDFPTVF GKISSSTKIW KPLAQTRSIM QPKTVFPPLT QIKLQRYPES
1260 1270 1280 1290 1300
AEEKVKVEPL DSLSLFHLKT ESNGKAFTDK AYNSQVQLTV NANQKAHPLT
1310 1320 1330 1340 1350
QPSSPPNQCA NVMAGDDQIR FQQVVKEQLM HQRLPTLPGI SHETPLPESA
1360 1370 1380 1390 1400
LTLRNVNVVC SGGITVVSTK SEEEVCSSSF GTSEFSTVDS AQKNFNDYAM
1410 1420 1430 1440 1450
NFFTNPTKNL VSITKDSELP TCSCLDRVIQ KDKGPYYTHL GAGPSVAAVR
1460 1470 1480 1490 1500
EIMENRYGQK GNAIRIEIVV YTGKEGKSSH GCPIAKWVLR RSSDEEKVLC
1510 1520 1530 1540 1550
LVRQRTGHHC PTAVMVVLIM VWDGIPLPMA DRLYTELTEN LKSYNGHPTD
1560 1570 1580 1590 1600
RRCTLNENRT CTCQGIDPET CGASFSFGCS WSMYFNGCKF GRSPSPRRFR
1610 1620 1630 1640 1650
IDPSSPLHEK NLEDNLQSLA TRLAPIYKQY APVAYQNQVE YENVARECRL
1660 1670 1680 1690 1700
GSKEGRPFSG VTACLDFCAH PHRDIHNMNN GSTVVCTLTR EDNRSLGVIP
1710 1720 1730 1740 1750
QDEQLHVLPL YKLSDTDEFG SKEGMEAKIK SGAIEVLAPR RKKRTCFTQP
1760 1770 1780 1790 1800
VPRSGKKRAA MMTEVLAHKI RAVEKKPIPR IKRKNNSTTT NNSKPSSLPT
1810 1820 1830 1840 1850
LGSNTETVQP EVKSETEPHF ILKSSDNTKT YSLMPSAPHP VKEASPGFSW
1860 1870 1880 1890 1900
SPKTASATPA PLKNDATASC GFSERSSTPH CTMPSGRLSG ANAAAADGPG
1910 1920 1930 1940 1950
ISQLGEVAPL PTLSAPVMEP LINSEPSTGV TEPLTPHQPN HQPSFLTSPQ
1960 1970 1980 1990 2000
DLASSPMEED EQHSEADEPP SDEPLSDDPL SPAEEKLPHI DEYWSDSEHI
2010 2020 2030 2040 2050
FLDANIGGVA IAPAHGSVLI ECARRELHAT TPVEHPNRNH PTRLSLVFYQ
2060 2070 2080 2090 2100
HKNLNKPQHG FELNKIKFEA KEAKNKKMKA SEQKDQAANE GPEQSSEVNE
2110 2120 2130
LNQIPSHKAL TLTHDNVVTV SPYALTHVAG PYNHWV

Background

Function:

Dioxygenase that catalyzes the conversion of the modified genomic base 5-methylcytosine (5mC) into 5-hydroxymethylcytosine (5hmC) and plays a key role in active DNA demethylation. Also mediates subsequent conversion of 5hmC into 5-formylcytosine (5fC), and conversion of 5fC to 5-carboxylcytosine (5caC). Conversion of 5mC into 5hmC, 5fC and 5caC probably constitutes the first step in cytosine demethylation. Methylation at the C5 position of cytosine bases is an epigenetic modification of the mammalian genome which plays an important role in transcriptional regulation. In addition to its role in DNA demethylation, plays a more general role in chromatin regulation. Preferentially binds to CpG-rich sequences at promoters of both transcriptionally active and Polycomb-repressed genes. Involved in the recruitment of the O-GlcNAc transferase OGT to CpG-rich transcription start sites of active genes, thereby promoting histone H2B GlcNAcylation by OGT. Also involved in transcription repression of a subset of genes through recruitment of transcriptional repressors to promoters. Involved in the balance between pluripotency and lineage commitment of cells it plays a role in embryonic stem cells maintenance and inner cell mass cell specification. Plays an important role in the tumorigenicity of glioblastoma cells. TET1-mediated production of 5hmC acts as a recruitment signal for the CHTOP-methylosome complex to selective sites on the chromosome, where it methylates H4R3 and activates the transcription of genes involved in glioblastomagenesis (PubMed:25284789).

Post-translational Modifications:

Glycosylated. Interaction with OGT leads to GlcNAcylation (By similarity).

Subcellular Location:

Nucleus;

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte

Subunit Structure:

Interacts with HCFC1 and OGT (By similarity). Interacts with SIN3A; recruits the transcriptional corepressor SIN3A to gene promoters (PubMed:21490601). Found in a complex composed of at least SINHCAF, SIN3A, HDAC1, SAP30, RBBP4, OGT and TET1 (By similarity).

Similarity:

Belongs to the TET family.

Western blot analysis of extracts from EC304, using TET1 Antibody. The lane on the left was treated with blocking peptide.
Western blot analysis of extracts from A431 , using TET1 antibody. The lane on the left was treated with the antigen-specific peptide.
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Catalog Number :

DF6428-BP
(Blocking peptide available as DF6428-BP)

Price/Size :

$200/1mg.
Tips: For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Function :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

Format and storage :

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.

Precautions :

This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Pig
100%
Bovine
100%
Dog
100%
Zebrafish
60%
Chicken
0%
Rabbit
0%
Xenopus
0%
Horse
0%
Sheep
0%
High similarity Medium similarity Low similarity No similarity
Q8NFU7 as Substrate
Site PTM Type Enzyme
T30 Phosphorylation
T31 Phosphorylation
S45 Phosphorylation
S116 Phosphorylation
T189 Phosphorylation
S193 Phosphorylation
S322 Phosphorylation
S669 Phosphorylation
S805 Phosphorylation
S806 Phosphorylation
S871 Phosphorylation
Y935 Phosphorylation
S1155 Phosphorylation
K1161 Acetylation
S1228 Phosphorylation
T1234 Phosphorylation
S1304 Phosphorylation
T1382 Phosphorylation
K1589 Ubiquitination
Y1641 Phosphorylation
S1731 Phosphorylation
K1769 Ubiquitination
S1851 Phosphorylation
T1867 Phosphorylation
IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.