Caspase 7 Antibody | Affinity Biosciences

WB
IHC
Cites

1/3

Western blot analysis of extracts from rat muscle, using Caspase 7 Antibody.

DF6441 at 1/100 staining Rat lung tissue by IHC-P.

Product:	Caspase 7 Antibody
Catalog:	DF6441
Description:	Rabbit polyclonal antibody to Caspase 7
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.:	43 kDa; 34kD(Calculated).
Uniprot:	P55210
RRID:	AB_2838404

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(90%), Bovine(100%), Horse(90%), Sheep(100%), Rabbit(100%), Dog(100%)

Clonality:

Polyclonal

Specificity:

Caspase 7 Antibody detects endogenous levels of total Caspase 7.

RRID:

AB_2838404
Cite Format: Affinity Biosciences Cat# DF6441, RRID:AB_2838404.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Apoptotic protease Mch-3; Apoptotic protease MCH3; CASP-7; CASP7; CASP7_HUMAN; Caspase 7; Caspase 7 apoptosis related cysteine peptidase; Caspase-7 subunit p11; Caspase7; CMH 1; CMH-1; CMH1; ICE LAP3; ICE-LAP3; ICE-like apoptotic protease 3; LICE2; MCH3;

Immunogens

Immunogen:

Uniprot:

P55210(CASP7_HUMAN) >>Visit HPA database.

Gene(ID):

CASP7(840)

Expression:

P55210 CASP7_HUMAN:

Highly expressed in lung, skeletal muscle, liver, kidney, spleen and heart, and moderately in testis. No expression in the brain.

Description:

Caspase-7 (CMH-1, Mch3, ICE-LAP3) has been identified as a major contributor to the execution of apoptosis (1-4). Caspase-7, like caspase-3, is an effector caspase that is responsible for cleaving downstream substrates such as (ADP-ribose) polymerase and PARP (1,3). During apoptosis, caspase-7 is activated through proteolyticprocesssing by upstream caspases at Asp23, Asp198, and Asp206 to produce the mature subunits (1,3). Similar to caspases-2 and -3, caspase-7 preferentially cleaves substrates following the recognition sequence DEVD (5).

Sequence:

P55210 CASP7_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MADDQGCIEEQGVEDSANEDSVDAKPDRSSFVPSLFSKKKKNVTMRSIKTTRDRVPTYQYNMNFEKLGKCIIINNKNFDKVTGMGVRNGTDKDAEALFKCFRSLGFDVIVYNDCSCAKMQDLLKKASEEDHTNAACFACILLSHGEENVIYGKDGVTPIKDLTAHFRGDRCKTLLEKPKLFFIQACRGTELDDGIQADSGPINDTDANPRYKIPVEADFLFAYSTVPGYYSWRSPGRGSWFVQALCSILEEHGKDLEIMQILTRVNDRVARHFESQSDDPHFHEKKQIPCVVSMLTKELYFSQ

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Bovine

100

Sheep

100

Dog

100

Rabbit

100

Pig

Horse

Chicken

Xenopus

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P55210 As Substrate

P55210

Site	PTM Type	Enzyme	Source
A2	Acetylation		Uniprot
S16	Phosphorylation		Uniprot
S29	Phosphorylation		Uniprot
S30	Phosphorylation	Q13177 (PAK2)	Uniprot
S34	Phosphorylation		Uniprot
S37	Phosphorylation		Uniprot
K38	Methylation		Uniprot
K38	Ubiquitination		Uniprot
T44	Phosphorylation		Uniprot
S47	Phosphorylation		Uniprot
K69	Ubiquitination		Uniprot
K80	Ubiquitination		Uniprot
K92	Ubiquitination		Uniprot
K99	Ubiquitination		Uniprot
K153	Ubiquitination		Uniprot
T157	Phosphorylation		Uniprot
K160	Ubiquitination		Uniprot
K172	Ubiquitination		Uniprot
T173	Phosphorylation	Q13177 (PAK2)	Uniprot
K177	Ubiquitination		Uniprot
S234	Phosphorylation		Uniprot
S239	Phosphorylation	Q13177 (PAK2)	Uniprot

Research Backgrounds

P55210_CASP7_HUMAN

Function:

Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Overexpression promotes programmed cell death.

PTMs:

Cleavages by granzyme B or caspase-10 generate the two active subunits. Propeptide domains can also be cleaved efficiently by caspase-3. Active heterodimers between the small subunit of caspase-7 and the large subunit of caspase-3, and vice versa, also occur.

Subcellular Location:

Cytoplasm.

Tissue Specificity:

Highly expressed in lung, skeletal muscle, liver, kidney, spleen and heart, and moderately in testis. No expression in the brain.

Subunit Structure:

Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 20 kDa (p20) and a 11 kDa (p11) subunit. Interacts with BIRC6/bruce. Interacts with ATXN3 (short isoform 1).

Family&Domains:

Belongs to the peptidase C14A family.

Research Fields

· Cellular Processes > Cell growth and death > Apoptosis. (View pathway)

· Cellular Processes > Cell growth and death > Apoptosis - multiple species. (View pathway)

· Environmental Information Processing > Signal transduction > TNF signaling pathway. (View pathway)

· Human Diseases > Endocrine and metabolic diseases > Non-alcoholic fatty liver disease (NAFLD).

· Human Diseases > Neurodegenerative diseases > Alzheimer's disease.

· Human Diseases > Infectious diseases: Bacterial > Pertussis.

· Human Diseases > Infectious diseases: Bacterial > Legionellosis.

· Human Diseases > Cancers: Overview > Pathways in cancer. (View pathway)

References

1). Precise editing of FGFR3-TACC3 fusion genes with CRISPR-Cas13a in glioblastoma. Molecular Therapy (PubMed: 34274537) [IF=12.4]

2). Combination LSD1 and HOTAIR-EZH2 inhibition disrupts cell cycle processes and induces apoptosis in glioblastoma cells. PHARMACOLOGICAL RESEARCH (PubMed: 34246782) [IF=9.3]

Application: WB Species: Mice Sample: GBM cells

Fig. 5. Combination GSK-LSD1 + AQB pro- motes apoptosis by targeting BBC3 in vitro. (A) mRNA levels of BBC3 measured by qPCR after treatment 100 µM GSK-LSD1, 40 µM AQB, or 100 µM GSK-LSD1 + 40 µM AQB for 48 h. (B) Relative BBC3 mRNA levels after treat- ment with 100 µM GSK-LSD1, 40 µM AQB, or their combination after 12, 24, 48, or 72 h. (C) Relative BBC3 mRNA levels measured by qPCR after treatment with indicated concen- trations of AQB or GSK-LSD1 for 48 h. (D) Combination treatment effects on BBC3 mRNA levels at varied concentrations. (E) Western blot analysis of PUMA, Caspase 3, Caspase 7 after treatment with indicated concentrations of AQB for 48 h. (F) Western blot analysis of PUMA, Caspase 3, Caspase 7 after treatment with 200 µM GSK-LSD1, 80 µM AQB, or 200 µM GSK-LSD1 + 80 µM AQB for 48 h. (G) Apoptosis detection after 48 h of treatment with 200 µM GSK-LSD1, 80 µM AQB, or 200 µM GSK-LSD1 + 80 µM AQB. (H) Immunofluorescence assay for PUMA expression after the treatment with 100 µM GSK-LSD1, 40 µM AQB, or 100 µM GSK-LSD1 + 40 µM AQB for 48 h. Data are represented as mean ± s.d.; n = 3 indepen- dent experiments. ****P < 0.0001, ***P < 0.001, **P < 0.01, *P < 0.05.

3). CircGCN1L1 promotes synoviocyte proliferation and chondrocyte apoptosis by targeting miR-330-3p and TNF-α in TMJ osteoarthritis. Cell Death & Disease (PubMed: 32332704) [IF=9.0]

4). MSC-Derived Exosomes Ameliorate Intervertebral Disc Degeneration By Regulating the Keap1/Nrf2 Axis. Stem Cell Reviews and Reports (PubMed: 37528254) [IF=4.8]

5). 沉默小窝蛋白1对棕榈酸作用下的胰岛 β细胞存活的影响.. Chinese Journal of Diabetes Mellitus

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Caspase 7 Antibody - #DF6441