Product: CD4 Antibody
Catalog: DF6451
Source: Rabbit
Application: WB, ELISA(peptide)
Reactivity: Human, Mouse
Prediction: Pig, Horse, Rabbit, Dog
Mol.Wt.: 55kD; 51kD(Calculated).
Uniprot: P01730
RRID: AB_2838414

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, ELISA(peptide) 1:20000-1:40000
*The optimal dilutions should be determined by the end user.
Reactivity:
Human,Mouse
Prediction:
Pig(100%), Horse(100%), Rabbit(86%), Dog(100%)
Clonality:
Polyclonal
Specificity:
CD4 Antibody detects endogenous levels of total CD4.
RRID:
AB_2838414
Cite Format: Affinity Biosciences Cat# DF6451, RRID:AB_2838414.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CD 4; CD4 (L3T4); CD4; CD4 antigen (p55); CD4 antigen; CD4 molecule; CD4 receptor; CD4+ Lymphocyte deficiency, included; CD4_HUMAN; CD4mut; L3T4; Leu3; Ly-4; Lymphocyte antigen CD4; MGC165891; OTTHUMP00000238897; p55; T cell antigen T4; T cell antigen T4/LEU3; T cell differentiation antigen L3T4; T cell OKT4 deficiency, included; T cell surface antigen T4/Leu 3; T cell surface antigen T4/Leu3; T cell surface glycoprotein CD4; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4; W3/25; W3/25 antigen;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
P01730 CD4_HUMAN:

Highly expressed in T-helper cells. The presence of CD4 is a hallmark of T-helper cells which are specialized in the activation and growth of cytotoxic T-cells, regulation of B cells, or activation of phagocytes. CD4 is also present in other immune cells such as macrophages, dendritic cells or NK cells.

Description:
Cluster of Differentiation 4 (CD4) is a glycoprotein composed of an amino-terminal extracellular domain (four domains: D1-D4 with Ig-like structures), a transmembrane part and a short cytoplasmic tail. CD4 is expressed on the surface of T helper cells, regulatory T cells, monocytes, macrophages and dendritic cells, and plays an important role in the development and activation of T cells. On T cells, CD4 is the co-receptor for the T cell receptor (TCR), and these two distinct structures recognize the Antigen–Major Histocompatibility Complex (MHC). Specifically, the D1 domain of CD4 interacts with the β2-domain of the MHC class II molecule. CD4 ensures specificity of the TCR–antigen interaction, prolongs the contact between the T cell and the antigen presenting cell and recruits the tyrosine kinase Lck, which is essential for T cell activation (1).
Sequence:
MNRGVPFRHLLLVLQLALLPAATQGKKVVLGKKGDTVELTCTASQKKSIQFHWKNSNQIKILGNQGSFLTKGPSKLNDRADSRRSLWDQGNFPLIIKNLKIEDSDTYICEVEDQKEEVQLLVFGLTANSDTHLLQGQSLTLTLESPPGSSPSVQCRSPRGKNIQGGKTLSVSQLELQDSGTWTCTVLQNQKKVEFKIDIVVLAFQKASSIVYKKEGEQVEFSFPLAFTVEKLTGSGELWWQAERASSSKSWITFDLKNKEVSVKRVTQDPKLQMGKKLPLHLTLPQALPQYAGSGNLTLALEAKTGKLHQEVNLVVMRATQLQKNLTCEVWGPTSPKLMLSLKLENKEAKVSKREKAVWVLNPEAGMWQCLLSDSGQVLLESNIKVLPTWSTPVQPMALIVLGGVAGLLLFIGLGIFFCVRCRHRRRQAERMSQIKRLLSEKKTCQCPHRFQKTCSPI

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Dog
100
Rabbit
86
Bovine
0
Sheep
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P01730 As Substrate

Site PTM Type Enzyme
S67 Phosphorylation
T70 Phosphorylation
S74 Phosphorylation
S433 Phosphorylation P17252 (PRKCA)
K436 Ubiquitination
S440 Phosphorylation P17252 (PRKCA)
K453 Ubiquitination
S456 Phosphorylation

Research Backgrounds

Function:

Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class II molecule:peptide complex. The antigens presented by class II peptides are derived from extracellular proteins while class I peptides are derived from cytosolic proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class II presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK then initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of T-helper cells. In other cells such as macrophages or NK cells, plays a role in differentiation/activation, cytokine expression and cell migration in a TCR/LCK-independent pathway. Participates in the development of T-helper cells in the thymus and triggers the differentiation of monocytes into functional mature macrophages.

(Microbial infection) Primary receptor for human immunodeficiency virus-1 (HIV-1). Down-regulated by HIV-1 Vpu. Acts as a receptor for Human Herpes virus 7/HHV-7.

PTMs:

Palmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts.

Phosphorylated by PKC; phosphorylation at Ser-433 plays an important role for CD4 internalization.

Subcellular Location:

Cell membrane>Single-pass type I membrane protein.
Note: Localizes to lipid rafts (PubMed:12517957, PubMed:9168119). Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Highly expressed in T-helper cells. The presence of CD4 is a hallmark of T-helper cells which are specialized in the activation and growth of cytotoxic T-cells, regulation of B cells, or activation of phagocytes. CD4 is also present in other immune cells such as macrophages, dendritic cells or NK cells.

Subunit Structure:

Forms disulfide-linked homodimers at the cell surface. Interacts with LCK. Interacts with PTK2/FAK1. Binds to P4HB/PDI. Interacts with IL16; this interaction induces a CD4-dependent signaling in lymphocytes.

(Microbial infection) Interacts with HIV-1 Envelope polyprotein gp160 and protein Vpu.

(Microbial infection) Interacts with Human Herpes virus 7 surface proteins.

Research Fields

· Environmental Information Processing > Signaling molecules and interaction > Cell adhesion molecules (CAMs).   (View pathway)

· Human Diseases > Immune diseases > Primary immunodeficiency.

· Organismal Systems > Immune system > Antigen processing and presentation.   (View pathway)

· Organismal Systems > Immune system > Hematopoietic cell lineage.   (View pathway)

· Organismal Systems > Immune system > Th1 and Th2 cell differentiation.   (View pathway)

· Organismal Systems > Immune system > Th17 cell differentiation.   (View pathway)

· Organismal Systems > Immune system > T cell receptor signaling pathway.   (View pathway)

References

1). Shen LF et al. The role of GLUT-1 in upregulation of PD-L1 expression after radiotherapy and PD-L1 is associated with a favourable overall survival in hypopharyngeal cancer. Onco Targets Ther 2020 Nov 3;13:11221-11235. (PubMed: 33173312) [IF=3.337]

Application: IHC    Species: Human    Sample: tumor tissues

Figure 1 PD-L1 and Glut-1 expression, and CD4+, CD8+ T cells infiltration (the black arrows point) in HNSCC at baseline (pre-RT) and after RT (magnification ×400). Positive expression of PD-L1 pre-RT (A) and after RT (B); Positive expression of Glut-1 pre-RT (C) and after RT (D); CD4+ T cells infiltration in tumor tissues pre-RT (E) and after RT (F); CD8+ T cells infiltration in tumor tissues pre-RT (G) and after RT (H). Abbreviations: GLUT-1, glucose transporter 1; HNSCC, head and neck squamous cell carcinoma; PD-L1, programmed cell death ligand 1; RT, radiotherapy.

2). Shen LF et al. The role of GLUT-1 in upregulation of PD-L1 expression after radiotherapy and PD-L1 is associated with a favourable overall survival in hypopharyngeal cancer. Onco Targets Ther 2020 Nov 3;13:11221-11235. (PubMed: 33173312) [IF=3.337]

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