B2M Antibody | Affinity Biosciences

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Western blot analysis of extracts from 293, using B2M Antibody.

DF6458 at 1/100 staining Mouse testis tissue by IHC-P.

DF6458 at 1/100 staining Rat testis tissue by IHC-P.

Product:	B2M Antibody
Catalog:	DF6458
Description:	Rabbit polyclonal antibody to B2M
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit
Mol.Wt.:	14kDa; 14kD(Calculated).
Uniprot:	P61769
RRID:	AB_2838420

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(92%)

Clonality:

Polyclonal

Specificity:

B2M Antibody detects endogenous levels of total B2M.

RRID:

AB_2838420
Cite Format: Affinity Biosciences Cat# DF6458, RRID:AB_2838420.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

B2M; B2MG_HUMAN; Beta 2 microglobin; Beta 2 microglobulin; Beta 2 microglobulin precursor; Beta chain of mhc class 1 proteins; Beta chain of MHC class I molecules; Beta-2-microglobulin form pI 5.3; CDABP0092; Hdcma22p;

Immunogens

Immunogen:

Uniprot:

P61769(B2MG_HUMAN) >>Visit HPA database.

Gene(ID):

B2M(567)

Description:

β2-microglobulin (B2M) is a principal component of the Major Histocompatibility Complex (MHC) class I molecule, a ternary membrane protein complex that displays fragments derived from proteolyzed cytosolic proteins on the surface of cells for recognition by the surveillance immune system (1,2). As an integral component of the MHC class I complex, β2-microglobulin plays a critically important role in immune system function (3). It has important relevance to cancer biology research; for example, research studies have shown that nearly one-third of diffuse large B cell lymphomas contain mutations that inactivate β2-microglobulin gene function, thereby allowing tumor cells to escape immune detection (4). In additon, β2-microglobulin has been identified as an amyloid preprotein with collagen-binding affinity (5); its accumulation in osteoarthritic lesions of long-term dialysis patients is reportedly a contributing factor to the condition known as amyloid osteoarthropathy (6).

Sequence:

P61769 B2MG_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MSRSVALAVLALLSLSGLEAIQRTPKIQVYSRHPAENGKSNFLNCYVSGFHPSDIEVDLLKNGERIEKVEHSDLSFSKDWSFYLLYYTEFTPTEKDEYACRVNHVTLSQPKIVKWDRDM

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Rabbit

Chicken

Zebrafish

Dog

Xenopus

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P61769 As Substrate

P61769

Site	PTM Type	Source
M1	Acetylation	Uniprot
S4	Phosphorylation	Uniprot
S14	Phosphorylation	Uniprot
T24	Phosphorylation	Uniprot
K26	Ubiquitination	Uniprot
Y30	Phosphorylation	Uniprot
S31	Phosphorylation	Uniprot
K39	Ubiquitination	Uniprot
C45	S-Nitrosylation	Uniprot
K61	Ubiquitination	Uniprot
K68	Ubiquitination	Uniprot
S72	Phosphorylation	Uniprot
S75	Phosphorylation	Uniprot
S77	Phosphorylation	Uniprot
K78	Methylation	Uniprot
T106	O-Glycosylation	Uniprot
S108	Phosphorylation	Uniprot
K111	Ubiquitination	Uniprot
K114	Ubiquitination	Uniprot

Research Backgrounds

P61769_B2MG_HUMAN

Function:

Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system. Exogenously applied M.tuberculosis EsxA or EsxA-EsxB (or EsxA expressed in host) binds B2M and decreases its export to the cell surface (total protein levels do not change), probably leading to defects in class I antigen presentation.

PTMs:

Glycation of Ile-21 is observed in long-term hemodialysis patients.

Subcellular Location:

Secreted. Cell surface.
Note: Detected in serum and urine (PubMed:1336137, PubMed:7554280).

Note: (Microbial infection) In the presence of M.tuberculosis EsxA-EsxB complex decreased amounts of B2M are found on the cell surface (PubMed:25356553).

Subunit Structure:

Heterodimer of an alpha chain and a beta chain. Beta-2-microglobulin is the beta-chain of major histocompatibility complex class I molecules. Polymers of beta 2-microglobulin can be found in tissues from patients on long-term hemodialysis. B2M alone (not in complex with HLA-I) interacts with M.tuberculosis EsxA (ESAT-6) and an EsxA-EsxB (CFP-10) complex; the tripartite complex can be detected in the host endoplasmic reticulum. The B2M-EsxA complex can be detected in patients with pleural tuberculosis and is stable from pH 4.0 to 8.0 and in the presence of 2M NaCl. Forms a heterotrimer with HLA-E and a self- or a foreign peptide. Forms a heterotrimer with HLA-G and a self-peptide. Forms a heterotrimer with HLA-F and a self-peptide.

Family&Domains:

Belongs to the beta-2-microglobulin family.

Research Fields

· Organismal Systems > Immune system > Antigen processing and presentation. (View pathway)

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B2M Antibody - #DF6458