NPPA Antibody - #DF6497

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Product: NPPA Antibody
Catalog: DF6497
Description: Rabbit polyclonal antibody to NPPA
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
Mol.Wt.: 17kDa; 16kD(Calculated).
Uniprot: P01160
RRID: AB_2838459

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 100ul $280 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(91%)
Clonality:
Polyclonal
Specificity:
NPPA Antibody detects endogenous levels of total NPPA.
RRID:
AB_2838459
Cite Format: Affinity Biosciences Cat# DF6497, RRID:AB_2838459.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ANF; ANF_HUMAN; ANP; ATFB6; Atrial natriuretic factor; Atrial natriuretic peptide; Atriopeptin; Cardiodilatin; Cardiodilatin-related peptide; Cardionatrin; CDD ANF; CDD; CDD-ANF; CDP; Natriuretic peptide A; Natriuretic peptide precursor A; NPPA; PND; Prepronatriodilatin; ANP;

Immunogens

Immunogen:
Uniprot:

P01160(ANF_HUMAN) >>Visit HPA database.

Gene(ID):
NPPA(4878)
Description:
The protein encoded by this gene belongs to the natriuretic peptide family. Natriuretic peptides are implicated in the control of extracellular fluid volume and electrolyte homeostasis. This protein is synthesized as a large precursor (containing a signal peptide), which is processed to release a peptide from the N-terminus with similarity to vasoactive peptide, cardiodilatin, and another peptide from the C-terminus with natriuretic-diuretic activity. Mutations in this gene have been associated with atrial fibrillation familial type 6. [provided by RefSeq, Sep 2009]
Sequence:
MSSFSTTTVSFLLLLAFQLLGQTRANPMYNAVSNADLMDFKNLLDHLEEKMPLEDEVVPPQVLSEPNEEAGAALSPLPEVPPWTGEVSPAQRDGGALGRGPWDSSDRSALLKSKLRALLTAPRSLRRSSCFGGRMDRIGAQSGLGCNSFRY

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
91
Zebrafish
73
Chicken
70
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P01160 As Substrate

Site PTM Type Enzyme
K41 Acetylation
Y151 Phosphorylation

Research Backgrounds

Function:

Hormone playing a key role in cardiovascular homeostasis through regulation of natriuresis, diuresis, and vasodilation. Also plays a role in female pregnancy by promoting trophoblast invasion and spiral artery remodeling in uterus. Specifically binds and stimulates the cGMP production of the NPR1 receptor. Binds the clearance receptor NPR3.

PTMs:

Cleaved by CORIN upon secretion to produce the functional hormone.

Atrial natriuretic factor: Cleaved by MME. The cleavage initiates degradation of the factor and thereby regulate its activity.

Subcellular Location:

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Family&Domains:

Belongs to the natriuretic peptide family.

Research Fields

· Environmental Information Processing > Signal transduction > HIF-1 signaling pathway.   (View pathway)

References

1). Ormeloxifene, a selective estrogen receptor modulator, protects against pulmonary hypertension. European Journal of Pharmacology (PubMed: 36731722) [IF=5.0]
2). Metformin attenuates angiotensin II‑induced cardiomyocyte hypertrophy by upregulating the MuRF1 and MAFbx pathway. Experimental and Therapeutic Medicine (PubMed: 34539827) [IF=2.7]

Application: WB    Species: Rat    Sample: H9c2 cells

Figure 1 Effect of metformin on cardiomyocyte hypertrophy. (A) Metformin reversed the changes in the surface area of cardiomyocytes induced by Ang II treatment for 48 h. Metformin reversed the changes in (B) ANP and (C) BNP mRNA expression in cardiomyocytes after treatment with Ang II for 48 h. (D) Western blot analysis of ANP and BNP. (E) Densitometric analysis of ANP and BNP expression in cardiomyocytes. n=3 for each group. *P<0.05. #P<0.05. ANP, atrial natriuretic peptide; BNP, brain natriuretic peptide; Ang, angiotensin; Met, metformin.

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Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

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