PDE4D Antibody - #DF6535

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Product: PDE4D Antibody
Catalog: DF6535
Description: Rabbit polyclonal antibody to PDE4D
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 91kDa; 91kD(Calculated).
Uniprot: Q08499
RRID: AB_2838497

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MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)
Clonality:
Polyclonal
Specificity:
PDE4D Antibody detects endogenous levels of total PDE4D.
RRID:
AB_2838497
Cite Format: Affinity Biosciences Cat# DF6535, RRID:AB_2838497.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

cAMP specific 3',5' cyclic phosphodiesterase 4D; DKFZp686M11213; 5''-cyclic phosphodiesterase 4D; cAMP specific phosphodiesterase 4D; cAMP specific phosphodiesterase PDE4D6; cAMP-specific 3''; DPDE3; Dunce like phosphodiesterase E3; FLJ97311; HSPDE4D; PDE43; Pde4d; PDE4D_HUMAN; PDE4DN2; Phosphodiesterase 4D cAMP specific (dunce (Drosophila) homolog phosphodiesterase E3); Phosphodiesterase 4D cAMP specific (phosphodiesterase E3 dunce homolog Drosophila); Phosphodiesterase 4D cAMP specific; Phosphodiesterase 4D, cAMP specific (dunce); STRK1;

Immunogens

Immunogen:
Uniprot:

Q08499(PDE4D_HUMAN) >>Visit HPA database.

Gene(ID):
PDE4D(5144)
Expression:
Q08499 PDE4D_HUMAN:

Expressed in colonic epithelial cells (at protein level). Widespread; most abundant in skeletal muscle. Isoform 6 is detected in brain. Isoform 8 is detected in brain, placenta, lung and kidney. Isoform 7 is detected in heart and skeletal muscle.

Description:
PDE4D(cAMP-specific 3',5'-cyclic phosphodiesterase 4D) is also named as DPDE3, PDE43 and belongs to the cyclic nucleotide phosphodiesterase family and PDE4 subfamily. PDE4D subtype may be of particular importance as an antidepressant target in that it is regulated by repeated treatment with both norepinephrine and serotonin reuptake inhibitors as well as by the PDE4 inhibitor rolipram(PMID:16730340). It has at least 9 isoforms and all 9 PDE4D isoforms are expressed in healthy adult human hippocampus and in hippocampus from a patient with advanced Alzheimer disease(PMID:17353396). This antibody is specific to PDE4D.
Sequence:
MEAEGSSAPARAGSGEGSDSAGGATLKAPKHLWRHEQHHQYPLRQPQFRLLHPHHHLPPPPPPSPQPQPQCPLQPPPPPPLPPPPPPPGAARGRYASSGATGRVRHRGYSDTERYLYCRAMDRTSYAVETGHRPGLKKSRMSWPSSFQGLRRFDVDNGTSAGRSPLDPMTSPGSGLILQANFVHSQRRESFLYRSDSDYDLSPKSMSRNSSIASDIHGDDLIVTPFAQVLASLRTVRNNFAALTNLQDRAPSKRSPMCNQPSINKATITEEAYQKLASETLEELDWCLDQLETLQTRHSVSEMASNKFKRMLNRELTHLSEMSRSGNQVSEFISNTFLDKQHEVEIPSPTQKEKEKKKRPMSQISGVKKLMHSSSLTNSSIPRFGVKTEQEDVLAKELEDVNKWGLHVFRIAELSGNRPLTVIMHTIFQERDLLKTFKIPVDTLITYLMTLEDHYHADVAYHNNIHAADVVQSTHVLLSTPALEAVFTDLEILAAIFASAIHDVDHPGVSNQFLINTNSELALMYNDSSVLENHHLAVGFKLLQEENCDIFQNLTKKQRQSLRKMVIDIVLATDMSKHMNLLADLKTMVETKKVTSSGVLLLDNYSDRIQVLQNMVHCADLSNPTKPLQLYRQWTDRIMEEFFRQGDRERERGMEISPMCDKHNASVEKSQVGFIDYIVHPLWETWADLVHPDAQDILDTLEDNREWYQSTIPQSPSPAPDDPEEGRQGQTEKFQFELTLEEDGESDTEKDSGSQVEEDTSCSDSKTLCTQDSESTEIPLDEQVEEEAVGEEEESQPEACVIDDRSPDT

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Zebrafish
100
Chicken
100
Rabbit
100
Xenopus
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q08499 As Substrate

Site PTM Type Enzyme
S18 Phosphorylation
S20 Phosphorylation
T25 Phosphorylation
S142 Phosphorylation
S171 Phosphorylation
S174 Phosphorylation
S190 Phosphorylation
S195 Phosphorylation
S197 Phosphorylation
Y199 Phosphorylation
S202 Phosphorylation
S214 Phosphorylation
S252 Phosphorylation
S255 Phosphorylation
S262 Phosphorylation
S299 Phosphorylation
S301 Phosphorylation
K307 Ubiquitination
K309 Methylation
T317 Phosphorylation
S325 Phosphorylation
S330 Phosphorylation
S334 Phosphorylation
K340 Ubiquitination
S348 Phosphorylation
K358 Ubiquitination
S362 Phosphorylation
S365 Phosphorylation
S375 Phosphorylation
K387 Sumoylation
K387 Ubiquitination
K396 Ubiquitination
K556 Ubiquitination
S576 Phosphorylation
S596 Phosphorylation
S597 Phosphorylation
S606 Phosphorylation
S657 Phosphorylation
S715 Phosphorylation P27361 (MAPK3) , P28482 (MAPK1)
S746 Phosphorylation
S761 Phosphorylation

Research Backgrounds

Function:

Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes.

PTMs:

Long isoforms that share a conserved PKA phosphorylation site in the N-terminus are activated by PKA through phosphorylation (By similarity). Isoform 3 and isoform 7 are activated by phosphorylation (in vitro), but not isoform 6. Isoform N3 and isoform 12 are phosphorylated on Ser-49, Ser-51, Ser-55 and Ser-59.

Sumoylation of long isoforms by PIAS4 augments their activation by PKA phosphorylation and represses their inhibition by ERK phosphorylation.

Subcellular Location:

Apical cell membrane. Cytoplasm. Membrane. Cytoplasm>Cytoskeleton. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome.
Note: Found in the soluble fraction, associated with membranes, and associated with the cytoskeleton and the centrosome (By similarity). Colocalized with SHANK2 to the apical membrane of colonic crypt cells.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in colonic epithelial cells (at protein level). Widespread; most abundant in skeletal muscle. Isoform 6 is detected in brain. Isoform 8 is detected in brain, placenta, lung and kidney. Isoform 7 is detected in heart and skeletal muscle.

Subunit Structure:

Homodimer for the long isoforms. Isoforms with truncated N-termini are monomeric. Isoform 3 is part of a ternary complex containing PRKAR2A, PRKAR2B and AKAP9. Interacts with PDE4DIP. Identified in a complex composed of RYR1, PDE4D, PKA, FKBP1A and protein phosphatase 1 (PP1) (By similarity). Isoform 5, isoform N3 and isoform 12 bind RACK1 via their unique N-terminus. Binds ARRB2. Interacts (via N-terminal region) with SHANK2 (via proline-rich region); the interaction is increased in a PKA-dependent manner.

Family&Domains:

Belongs to the cyclic nucleotide phosphodiesterase family. PDE4 subfamily.

Research Fields

· Environmental Information Processing > Signal transduction > cAMP signaling pathway.   (View pathway)

· Human Diseases > Substance dependence > Morphine addiction.

· Metabolism > Nucleotide metabolism > Purine metabolism.

References

1). Resveratrol prevents Drp1-mediated mitochondrial fission in the diabetic kidney through the PDE4D/PKA pathway. Phytotherapy Research (PubMed: 37767771) [IF=7.2]
2). Moderate-Intensity Intermittent Training Alters the DNA Methylation Pattern of PDE4D Gene in Hippocampus to Improve the Ability of Spatial Learning and Memory in Aging Rats Reduced by D-Galactose. Brain Sciences (PubMed: 36979232) [IF=3.3]

Application: WB    Species: Rat    Sample:

Figure 4 Effects of MIIT on the expression of PDE4A/B/D mRNA and protein in aging rats: (a) The expression of PDE4A/B/D mRNA in hippocampus was detected by Real-Time PCR (mean ± SEM, n = 6). (b–e) Western blot analysis of PDE4A/B/D protein expression levels (mean ± SEM, n = 3). Independent-Samples t Test, * p < 0.05 vs. D-Gal group, ** p < 0.01 vs. D-Gal group. Control: young group treated with saline, D-Gal Group: aging group induced by D-galactose, D-Gal+E Group: aging group induced by D-galactose that performed treadmill exercise.

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