Product: Claudin 3 Antibody
Catalog: AF0129
Description: Rabbit polyclonal antibody to Claudin 3
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Sheep, Dog, Xenopus
Mol.Wt.: 25kDa; 23kD(Calculated).
Uniprot: O15551
RRID: AB_2833313

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 100ul $280 In stock
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Product Info

Source:
Rabbit
Application:
WB 1:500-1:3000, IHC 1:50-1:200, IF/ICC: 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(88%), Bovine(88%), Sheep(88%), Dog(88%), Xenopus(88%)
Clonality:
Polyclonal
Specificity:
Claudin 3 Antibody detects endogenous levels of total Claudin 3.
RRID:
AB_2833313
Cite Format: Affinity Biosciences Cat# AF0129, RRID:AB_2833313.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

C7orf1; Claudin-3; Claudin3; CLD3_HUMAN; CLDN 3; Cldn3; Clostridium perfringens enterotoxin receptor 2; CPE R2; CPE receptor 2; CPE-R 2; CPE-receptor 2; CPETR 2; CPETR2; HRVP 1; HRVP1; Rat ventral prostate 1 like protein; Rat ventral prostate.1 protein homolog; RVP1; Ventral prostate.1 like protein; Ventral prostate.1 protein homolog;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Description:
claudin 3 Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium- independent cell-adhesion activity. Belongs to the claudin family. Can form homo- and heteropolymers with other CLDN. Homopolymers interact with CLDN1 and CLDN2 homopolymers. Directly interacts with TJP1/ZO-1, TJP2/ZO-2 and TJP3/ZO-3.
Sequence:
MSMGLEITGTALAVLGWLGTIVCCALPMWRVSAFIGSNIITSQNIWEGLWMNCVVQSTGQMQCKVYDSLLALPQDLQAARALIVVAILLAAFGLLVALVGAQCTNCVQDDTAKAKITIVAGVLFLLAALLTLVPVSWSANTIIRDFYNPVVPEAQKREMGAGLYVGWAAAALQLLGGALLCCSCPPREKKYTATKVVYSAPRSTGPGASLGTGYDRKDYV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
88
Bovine
88
Sheep
88
Dog
88
Xenopus
88
Chicken
57
Horse
0
Zebrafish
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O15551 As Substrate

Site PTM Type Enzyme
Y191 Phosphorylation
T192 Phosphorylation P17612 (PRKACA)
T194 Phosphorylation
K195 Ubiquitination
Y198 Phosphorylation
S199 Phosphorylation
S203 Phosphorylation
T204 Phosphorylation
S209 Phosphorylation
T212 Phosphorylation
Y214 Phosphorylation
Y219 Phosphorylation

Research Backgrounds

Function:

Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity.

Subcellular Location:

Cell junction>Tight junction. Cell membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Can form homo- and heteropolymers with other CLDN. Homopolymers interact with CLDN1 and CLDN2 homopolymers. Directly interacts with TJP1/ZO-1, TJP2/ZO-2 and TJP3/ZO-3 (By similarity).

Family&Domains:

Belongs to the claudin family.

Research Fields

· Cellular Processes > Cellular community - eukaryotes > Tight junction.   (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > Cell adhesion molecules (CAMs).   (View pathway)

· Human Diseases > Infectious diseases: Viral > Hepatitis C.

· Organismal Systems > Immune system > Leukocyte transendothelial migration.   (View pathway)

References

1). USP5 promotes epithelial-mesenchymal transition by stabilizing SLUG in hepatocellular carcinoma. Theranostics (PubMed: 30809294) [IF=12.4]

Application: WB    Species: human    Sample: PLC-PRF-5 and Hep3B cells

Figure 3. |USP5 promotes EMT in hepatocellular carcinoma. (A) Motif analysis of SLUG ChIP-Seq cited from ChIPBASE. (B) PLC-PRF-5 and Hep3B cells were treated with different amounts of USP5 siRNA. Cellular extracts were prepared for ChIP assays with anti-SLUG. (C) PLC-PRF-5 and Hep3B cells were transfected with E-cadherin - dependent reporter gene plasmids. Luciferase activity was measured when cells overexpressed or knocked down USP5. (D) WB analysis of USP5, SLUG and EMT related markers in PLC-PRF-5 and Hep3B cells under USP5 knocked down or overexpressed treatment.

2). Cartilage Oligomeric Matrix Protein promotes epithelial-mesenchymal transition by interacting with Transgelin in Colorectal Cancer. Theranostics (PubMed: 32754278) [IF=12.4]

Application: WB    Species: Human    Sample: HCT116 cells

Figure 4. COMP promotes EMT in colorectal cancer. (A) Cell phenotypic changes in cells treated with COMP siRNA and COMP overexpression. (B) WB analysis of COMP and EMT-related markers in HCT116 cells under COMP knockdown or overexpression. (C) Cell wound scratch assay of HCT116, HCT-8, and SW620 cells treated with COMP siRNA or COMP overexpression vectors. (D) Transwell assay of HCT116, HCT-8, and SW620 cells treated with COMP siRNA or COMP overexpression vectors. (E) Immunofluorescence assay of HCT116, HCT-8, and SW620 cells treated with COMP siRNA or COMP overexpression vectors. Image J software was used to analyze the relative intensity of E-cadherin and Vimentin. (F) HCT116 and HCT-8 cells with knocked down or overexpressed COMP were transplanted on nude mice. Tumor volumes were measured every 4 days. (G) Tumor weight in the control, COMP knockdown, and COMP overexpression groups. (H) The in situ spleen model of the colorectal cancer cell line SW620 showed that overexpression of COMP promoted liver metastasis of colorectal cancer, while downregulation of COMP inhibited liver metastasis of colorectal cancer. (I) IHC staining to identify EMT biomarkers and COMP-related proteins in the control, COMP knockdown, and COMP overexpression groups. COMP knockdown displayed strong E-cadherin staining and reduced Vimentin, Snail1, Twist1, and MMP-2 staining. The expression levels of other proteins were identical to those observed in WB experiments. (J) Staining indices of COMP, E-cadherin, Vimentin, Snail1, Twist1, and MMP2. The error bars in all graphs represented SD, and each experiment was repeated three times. * and ** stand for P<0.05 and P<0.01, respectively.

3). Gut microbiota-mediated secondary bile acid alleviates Staphylococcus aureus-induced mastitis through the TGR5-cAMP-PKA-NF-κB/NLRP3 pathways in mice. npj Biofilms and Microbiomes (PubMed: 36755021) [IF=9.2]

4). Commensal cow Roseburia reduces gut-dysbiosis-induced mastitis through inhibiting bacterial translocation by producing butyrate in mice. Cell Reports (PubMed: 36417859) [IF=8.8]

Application: WB    Species: Mouse    Sample:

Figure 5Commensal Roseburia ameliorates gut-dysbiosis-induced mastitis in mice

5). Human menstrual blood-derived stem cell transplantation suppresses liver injury in DDC-induced chronic cholestasis. Stem Cell Research & Therapy (PubMed: 35123555) [IF=7.5]

Application: WB    Species: Mice    Sample: liver tissue

Fig. 5 MenSCs promoted TJ- and bile transport function-related protein expression and reduced fibrosis-related protein expression. A Western blot analysis protein levels of Claudin-1, Claudin-3, Claudin-5, Claudin-7 and Occludin in liver tissue of different groups (n = 3 for each group). B BSEP, OATP2 and NTCP1 expression in liver tissues of different groups (n = 3 for each group). C, D Liver COL1A1, α-SMA, TGF-β1 and β-catenin expression among different groups (n = 3 for each group). *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001

6). A fiber-enriched diet alleviates Staphylococcus aureus-induced mastitis by activating the HDAC3-mediated antimicrobial program in macrophages via butyrate production in mice. PLoS Pathogens (PubMed: 36656870) [IF=6.7]

7). Porcine Reproductive and Respiratory Syndrome Virus Structural Protein GP3 Regulates Claudin 4 To Facilitate the Early Stages of Infection. Journal of Virology (PubMed: 32759320) [IF=5.4]

Application: WB    Species: monkey    Sample: MARC-145 cells

FIG 1 CLDN4 plays a role in PRRSV infection. (A) Changes in TJ mRNA expression during TA-12 infection of MARC-145 cells. MARC-145 cells were infected with PRRSV TA-12, and the mRNAs were collected at 0, 12, 24, 36, and 48 hpi. The levels of CLDN3, CLDN4, OCLN, ZO-1, ZO-2, and JAM-1 mRNA were measured by relative qPCR. (B) Western blot analysis of the effects of PRRSV infection on CLDN4. MARC-145 cells were infected with TA-12, and the cells were collected at 12, 24, 36, and 48 hpi. The cellular proteins were analyzed by Western blotting using anti-CLDN4, anti-CLDN3, and anti-N antibodies. (C) Changes in CLDN4 expression in PAMs during TA-12 infection. PAMs were infected with PRRSV TA-12, and the cells were collected at 0, 12, 24, 36, and 48 hpi. The levels of mRNA and protein expression of CLDN4 were measured by relative qPCR and Western blotting, respectively. β-Actin was used as an internal control. The asterisks indicate significant differences relative to the control values: *, P 

8). Regulation of Tight Junctions by Sex Hormones in Goat Mammary Epithelial Cells. Animals (PubMed: 35681868) [IF=3.0]

Application: WB    Species: goat    Sample: goat mammary glands

Figure 1 The serum E2 and P4 levels and the protein expression of the TJ proteins of goats at the stages of 8 m, Pd91, Pd137, Ld4, and Ld31. (A) Serum E2 and P4 levels and the ratios of E2 to P4. (B) The protein expression of claudin-1, claudin-3, occludin, and ZO-1 in goat mammary glands. Data are presented as the means ± SD, different letters indicate the hormones are different (p < 0.05).

9). Sesquiterpene binding Gly-Leu-Ser/Lys-"co-adaptation pocket" to inhibit lung cancer cell epithelial-mesenchymal transition. Oncotarget (PubMed: 29050271)

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Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

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