Peroxiredoxin 1 Antibody - #DF6652

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Product: Peroxiredoxin 1 Antibody
Catalog: DF6652
Description: Rabbit polyclonal antibody to Peroxiredoxin 1
Application: WB IHC IF/ICC
Cited expt.: WB
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 22kDa(Observed); 22kD(Calculated).
Uniprot: Q06830
RRID: AB_2838614

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-400
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
Peroxiredoxin 1 Antibody detects endogenous levels of total Peroxiredoxin 1.
RRID:
AB_2838614
Cite Format: Affinity Biosciences Cat# DF6652, RRID:AB_2838614.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Heme binding 23 kDa protein; MSP23; Natural killer cell-enhancing factor A; NKEF A; NKEF-A; NKEFA; OSF3; Osteoblast specific factor 3; PAG; Paga; PAGB; Peroxiredoxin-1; PRDX1; PRDX1_HUMAN; Proliferation associated gene A; Proliferation-associated gene protein; PRX1; PrxI; TDPX2; Thioredoxin peroxidase 2; Thioredoxin-dependent peroxide reductase 2;

Immunogens

Immunogen:

A synthesized peptide derived from human Peroxiredoxin 1, corresponding to a region within the internal amino acids.

Uniprot:

Q06830(PRDX1_HUMAN) >>Visit HPA database.

Gene(ID):
PRDX1(5052)
Description:
This gene encodes a member of the peroxiredoxin family of antioxidant enzymes, which reduce hydrogen peroxide and alkyl hydroperoxides. The encoded protein may play an antioxidant protective role in cells, and may contribute to the antiviral activity of CD8(+) T-cells. This protein may have a proliferative effect and play a role in cancer development or progression. Four transcript variants encoding the same protein have been identified for this gene.
Sequence:
MSSGNAKIGHPAPNFKATAVMPDGQFKDISLSDYKGKYVVFFFYPLDFTFVCPTEIIAFSDRAEEFKKLNCQVIGASVDSHFCHLAWVNTPKKQGGLGPMNIPLVSDPKRTIAQDYGVLKADEGISFRGLFIIDDKGILRQITVNDLPVGRSVDETLRLVQAFQFTDKHGEVCPAGWKPGSDTIKPDVQKSKEYFSKQK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
63
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

Function:

Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides and as sensor of hydrogen peroxide-mediated signaling events. Might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H(2)O(2). Reduces an intramolecular disulfide bond in GDPD5 that gates the ability to GDPD5 to drive postmitotic motor neuron differentiation (By similarity).

PTMs:

Phosphorylated on Thr-90 during the M-phase, which leads to a more than 80% decrease in enzymatic activity.

The enzyme can be inactivated by further oxidation of the cysteine sulfenic acid (C(P)-SOH) to sulphinic acid (C(P)-SO2H) instead of its condensation to a disulfide bond. It can be reactivated by forming a transient disulfide bond with sulfiredoxin SRXN1, which reduces the cysteine sulfinic acid in an ATP- and Mg-dependent manner.

Subcellular Location:

Cytoplasm. Melanosome.
Note: Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Family&Domains:

Belongs to the peroxiredoxin family. AhpC/Prx1 subfamily.

Research Fields

· Cellular Processes > Transport and catabolism > Peroxisome.   (View pathway)

References

1). Anemoside B4 alleviates DSS-induced colitis by inhibiting CD1d-dependent NLRP3 inflammasome activation in macrophages. Authorea, 2023

Application: WB    Species: Mouse    Sample: BMDMs

Figure 6. AB4 inactivates AKT-STAT1-PRDX1-NF-κB signaling in macrophages. BMDMs (A ) and differentiated THP-1 cells (B ) were pretreated with AB4 (50, 100, and 200μM) for 4h, and then cultured with LPS (1μg/ml) for 6h. The protein of the indicated molecule was detected by Western Blot. BMDMs were pretreated with AB4 (200μM) or NF-κB inhibitor JSH-23 (25μM) for 4h and then cultured with LPS (1μg/ml) for 6h. The protein of the indicated molecule was detected by Western Blot (C ), and the mRNA expression of NLRP3, IL-1β, and IL-18 was analyzed by qPCR (D ). (E ) BMDMs were pretreated with AB4 (200μM) or AKT inhibitor MK2206 (20μM) for 4h and then cultured with LPS (1μg/ml) for 6h. The protein of the indicated molecule was detected by Western Blot. Data are presented as mean ± SD.**P < 0.01 vs. Control group;#P < 0.05, ##P < 0.01 vs. LPS group. (F ) BMDMs were pretreated with AB4 (200μM) for 4h or AKT agonist SC79 (20μM) for 6h and then cultured with LPS (1μg/ml) for 6h. The protein of the indicated molecule was detected by Western Blot. Data are presented as mean ± SD.**P < 0.01 LPS+SC79+AB4 vs. LPS+AB4 group. (G ) WT mice were subjected to DSS-induced colitis. The protein expression of the indicated molecule in colons was detected by Western Blot. Data are presented as mean ± SD. **P < 0.01 vs.Normal group; #P < 0.05,##P < 0.01 vs. DSS group.

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