ODC1 Antibody - #DF6712

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Product: ODC1 Antibody
Catalog: DF6712
Description: Rabbit polyclonal antibody to ODC1
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 51kDa; 51kD(Calculated).
Uniprot: P11926
RRID: AB_2838674

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(83%), Zebrafish(83%), Bovine(92%), Horse(100%), Sheep(100%), Rabbit(92%), Dog(100%), Chicken(100%), Xenopus(92%)
Clonality:
Polyclonal
Specificity:
ODC1 Antibody detects endogenous levels of total ODC1.
RRID:
AB_2838674
Cite Format: Affinity Biosciences Cat# DF6712, RRID:AB_2838674.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

DCOR_HUMAN; Dodc1; Odc 1; ODC; Odc1; ODC2; Ornithine decarboxylase 1; Ornithine decarboxylase 2; Ornithine decarboxylase; Ornithine decarboxylase structural 1; RNODC;

Immunogens

Immunogen:
Uniprot:

P11926(DCOR_HUMAN) >>Visit HPA database.

Gene(ID):
ODC1(4953)
Description:
Ornithine decarboxylase (ODC) is also named as ODC1and belongs to the Orn/Lys/Arg decarboxylase class-II family. It catalyzes the conversion of ornithine to putrescine, the first step and a major site of regulation of polyamine biosynthesis. The level of ODC is known to be controlled at several sites, namely transcription, translation, and enzyme degradation. Polyamines can stimulate the degradation of ODC as a type of negative feedback control(PMID:8486633). This protein can be phosphorylated in vivo(PMID:8798774). This antibody is specific to ODC1.
Sequence:
MNNFGNEEFDCHFLDEGFTAKDILDQKINEVSSSDDKDAFYVADLGDILKKHLRWLKALPRVTPFYAVKCNDSKAIVKTLAATGTGFDCASKTEIQLVQSLGVPPERIIYANPCKQVSQIKYAANNGVQMMTFDSEVELMKVARAHPKAKLVLRIATDDSKAVCRLSVKFGATLRTSRLLLERAKELNIDVVGVSFHVGSGCTDPETFVQAISDARCVFDMGAEVGFSMYLLDIGGGFPGSEDVKLKFEEITGVINPALDKYFPSDSGVRIIAEPGRYYVASAFTLAVNIIAKKIVLKEQTGSDDEDESSEQTFMYYVNDGVYGSFNCILYDHAHVKPLLQKRPKPDEKYYSSSIWGPTCDGLDRIVERCDLPEMHVGDWMLFENMGAYTVAAASTFNGFQRPTIYYVMSGPAWQLMQQFQNPDFPPEVEEQDASTLPVSCAWESGMKRHRAACASASINV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Sheep
100
Dog
100
Chicken
100
Bovine
92
Xenopus
92
Rabbit
92
Pig
83
Zebrafish
83
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P11926 As Substrate

Site PTM Type Enzyme
K27 Ubiquitination
K37 Ubiquitination
K50 Ubiquitination
K57 Ubiquitination
K69 Ubiquitination
K78 Ubiquitination
K115 Ubiquitination
K141 Ubiquitination
K150 Ubiquitination
K161 Ubiquitination
K247 Ubiquitination
K261 Ubiquitination
S303 Phosphorylation
C360 S-Nitrosylation

Research Backgrounds

Function:

Catalyzes the first and rate-limiting step of polyamine biosynthesis that converts ornithine into putrescine, which is the precursor for the polyamines, spermidine and spermine. Polyamines are essential for cell proliferation and are implicated in cellular processes, ranging from DNA replication to apoptosis.

PTMs:

S-Nitrosylation inhibits the enzyme. S-Nitrosylated in vitro on 4 cysteine residues.

Subunit Structure:

Homodimer. Only the dimer is catalytically active, as the active sites are constructed of residues from both monomers (Probable). Does not form a heterodimer with AZIN2 (By similarity).

Family&Domains:

Belongs to the Orn/Lys/Arg decarboxylase class-II family.

Research Fields

· Metabolism > Amino acid metabolism > Arginine and proline metabolism.

· Metabolism > Metabolism of other amino acids > Glutathione metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

References

1). Dissecting the single-cell transcriptome network underlying esophagus non-malignant tissues and esophageal squamous cell carcinoma. eBioMedicine (PubMed: 34192657) [IF=11.1]

Application: IF/ICC    Species: Human    Sample: ESCC and non-malignant tissues

Fig. 3 The single-cell transcriptomes of epithelial cells in non-malignant and malignant esophagus. (a) The TSNE plot and overview of epithelial cells by the cell type and origin of the cells. (b) Canonical cell markers were used to label epithelial subtypes as represented in the TSNE plot. (c) The TSNE plot of epithelial cells by the CNV's levels of the cells. (d) Heatmap showing the expression of marker genes in malignant and non-malignant epithelial cells. (e) Violin plots showing the marker genes for each epithelial cell type. (f) Immunofluorescence staining of tumor (n = 8) (up) / normal (n = 8) (down) tissue-specific genes in ESCC and non-malignant tissues. Comparison of non-malignant and malignant esophagus was performed by Wilcoxon test. (g) Pseudotime analysis for malignant and non-malignant epithelial cells. (h) Differences in pathway activities scored per cell by GSVA between malignant and non-malignant epithelial cells. The scores of pathways are normalized.
2). Oesophageal squamous cell carcinoma–associated IL‐33 rewires macrophage polarization towards M2 via activating ornithine decarboxylase. CELL PROLIFERATION (PubMed: 33305406) [IF=8.5]

Application: WB    Species: human    Sample: M2 macrophages

FIGURE 3|IL-33 induces macrophage ornithine decarboxylase (ODC) activation. A, ODC and arginase 1 (ARG1) protein levels were determined by Western blot, and GAPDH was used as a reference control.

Application: IF/ICC    Species: human    Sample: M2 macrophages

FIGURE 3|IL-33 induces macrophage ornithine decarboxylase (ODC) activation. A, ODC and arginase 1 (ARG1) protein levels were determined by Western blot, and GAPDH was used as a reference control. B, Relative intensity of ODC and ARG1 protein. C, Immunofluorescence staining for IL-33–induced M2 macrophages. The green signal represents the staining of activated ODC, and the blue signal represents the DAPI-stained nuclei (scale bar = 20 μm). *P < .05; **P < .01; ***P < .001. Bars indicate mean and SEM of triplicate experiments and show a representative experiment of at least 3 independent experiments performed for each panel
3). Inhibitory Effect of Ursolic Acid on the Migration and Invasion of Doxorubicin-Resistant Breast Cancer. MOLECULES (PubMed: 35209071) [IF=4.6]

Application: WB    Species: Human    Sample: MCF-7/ADR Cells

Figure 5 The effects of different concentrations of UA alone or in combination with Dox on ODC and phosphorylation of Erk1/2, VEGF, MMP-9. (A): representative immunoreactive bands of ODC, VEGF, MMP-9, GAPDH, total and phosphorylated Erk1/2 using specific antibody. (B–E): representative quantification of ODC, phosphorylated Erk1/2, VEGF, MMP-9. (F): determination of MMP-9 activity. * p < 0.05, ** p < 0.01, *** p < 0.001 compared with MCF-7/ADR cells, ## p < 0.01, ### p < 0.001, comparison between MCF-7/ADR group and MCF-7 group.

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